Analysis of nanomaterial biocoronas in biological and environmental surroundings
Nature Protocols,
Год журнала:
2024,
Номер
19(10), С. 3000 - 3047
Опубликована: Июль 23, 2024
Язык: Английский
Detailed Profiling of Protein Corona Formed by Polydopamine Nanoparticles in Human Plasma
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
The
term
protein
corona
(PC)
indicates
proteins
adsorbed
onto
the
surface
of
nanostructures
exposed
to
biological
media
such
as
blood
or
serum.
analysis
composition,
evolution,
and
effect
PC
complexed
with
nanomaterials
gained
attention
in
recent
years
due
importance
these
parameters
determining
fate
nanostructures.
In
particular,
represents
first
component
a
nanomaterial
interfacing
structures,
dictating
nanoparticle
internalization,
immune
response,
bioavailability,
even
toxicity.
Polydopamine
nanoparticles
(PDNPs),
obtained
through
polymerization
dopamine,
are
"smart"
materials
characterized
by
high
biocompatibility,
antioxidant
capacities,
tunability
reactivity,
biodegradability,
ability
act
photothermal
conversion
agents
when
irradiated
near-infrared
(NIR)
light
source.
Despite
many
interesting
applications
PDNPs
currently
described
scientific
literature,
there
is
still
no
comprehensive
phenomenon
formation
consequent
exposure
media.
Moreover,
date,
investigation
effects
irradiation
photothermally
active
on
composition
evolution
associated
has
been
extremely
limited.
With
this
work,
we
aim
provide
for
time
an
PDNPs,
before
after
NIR
stimulation.
We
formed
following
human
plasma
analyzed
several
overall
quantity,
PDNP
size,
presence
functionalization,
time,
laser,
demonstrating
that
play
pivotal
role
resulting
composition.
Eventually,
showed
have
significantly
different
properties
respect
bare
showing
higher
internalization
rates
glioblastoma
cells,
absorption
value,
enhanced
abilities.
Язык: Английский
Extreme Tolerance of Nanoparticle‐Protein Corona to Ultra‐High Abundance Proteins Enhances the Depth of Serum Proteomics
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 22, 2025
Abstract
The
serum
nanoparticle‐protein
corona
(NPC)
provides
specific
disease
information,
thus
opening
a
new
avenue
for
high‐throughput
in‐depth
proteomics
to
facilitate
biomarker
discovery.
Yet,
little
is
known
about
the
interactions
between
NPs
and
proteins,
its
role
in
enhanced
depth
of
proteomics.
Herein,
series
protein
spike‐in
experiments
are
conducted
systematically
investigate
depletion
enrichment
during
NPC
formation.
Proteomic
concentration‐dependent,
exhibits
powerful
tolerance
ultra‐high
abundant
proteins.
In
addition,
protein‐protein
(PPI),
especially
those
involving
albumin,
play
pivotal
promoting
proteomic
depth.
Furthermore,
triple‐protein
assay
established
interrogate
relationship
binding
affinity
concentration.
formation
product
balancing
affinity,
concentration,
PPI.
Overall,
this
study
elucidates
how
achieve
gain
better
understanding
as
an
essential
tool
proteome
profiling.
Язык: Английский
Protein Corona of Nanoparticles: Isolation and Analysis
Chem & Bio Engineering,
Год журнала:
2024,
Номер
1(9), С. 757 - 772
Опубликована: Окт. 3, 2024
Nanoparticles
entering
biological
systems
or
fluids
inevitably
adsorb
biomolecules,
such
as
protein,
on
their
surfaces,
forming
a
protein
corona.
Ensuing,
the
corona
endows
nanoparticles
with
new
identity
and
impacts
interaction
between
systems.
Hence,
development
of
reliable
techniques
for
isolation
analysis
is
key
understanding
behaviors
nanoparticles.
First,
this
review
systematically
outlines
approach
isolating
corona,
including
centrifugation,
magnetic
separation,
size
exclusion
chromatography,
flow-field-flow
fractionation,
other
emerging
methods.
Next,
we
qualitative
quantitative
characterization
methods
Finally,
underscore
necessary
steps
to
advance
efficiency
fidelity
nanoparticle
surfaces.
We
anticipate
that
these
insights
into
methodologies
will
profoundly
influence
technologies
aimed
at
elucidating
bionano
interactions
role
in
various
biomedical
applications.
Язык: Английский