Lithium-doped calcium silicate cement regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration

Journal of Biological Engineering, Год журнала: 2025, Номер 19(1)

Опубликована: Янв. 6, 2025

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement enhance regeneration modulate immune microenvironment promote tissue repair. We synthesized LiCS ceramic powder performed comprehensive analyses its physicochemical properties, including phase composition, morphology, setting time, mechanical strength. The results demonstrated that incorporation lithium into significantly increased diametral tensile strength (DTS) facilitated hydroxyapatite formation compared with undoped silicate. In vitro assays revealed enhanced proliferation, adhesion, spread Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines increasing anti-inflammatory cytokines, promoting polarization macrophages towards M2 phenotype. presence Li also improved osteogenic differentiation WJMSCs, as evidenced elevated levels alkaline phosphatase osteocalcin expression. These findings underscore dual functional capabilities enhancing osteogenesis modulating environment, making it promising material for engineering

Язык: Английский

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Designer Micro-/Nanocrumpled MXene Multilayer Coatings Accelerate Osteogenesis and Regulate Macrophage Polarization

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(17), С. 21415 - 21426

Опубликована: Март 6, 2024

Effective tissue regeneration and immune responses are essential for the success of biomaterial implantation. Although interaction between synthetic materials biological systems is well-recognized, role surface topographical cues in regulating local osteoimmune microenvironment─specifically, their impact on host cells, dynamic interactions─remains underexplored. This study addresses this gap by investigating topography osteogenesis immunomodulation. We fabricated MXene/hydroxyapatite (HAP)-coated surfaces with controlled 2.5D nano-, submicro-, microscale patterns using our custom bottom-up patterning method. These engineered were employed to assess behavior osteoblast precursor cells macrophage polarization. Our results demonstrate that MXene/HAP-coated crumpled significantly influence osteogenic activity polarization: these notably enhanced cell spreading, proliferation, differentiation facilitated a shift macrophages toward an anti-inflammatory, prohealing M2 phenotype. The observed indicate physical from topographies, combined chemical MXene/HAP coatings, synergistically create favorable microenvironment. presents first evidence employing MXene/HAP-multilayer coated finely concurrently facilitate immunomodulation improved implant-to-tissue integration. tunable topographic coatings coupled facile scalable fabrication process make them widely applicable various biomedical purposes. highlight potential multilayer improve vivo performance fate implants modulating response at material interface.

Язык: Английский

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Applications and Progress of 3D Printed Bioceramic Scaffolds in Bone Tissue Repair and Immune Regulation

Ceramics International, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский

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Advances in the application and research of biomaterials in promoting bone repair and regeneration through immune modulation

Materials Today Bio, Год журнала: 2024, Номер 30, С. 101410 - 101410

Опубликована: Дек. 16, 2024

Язык: Английский

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Angiogenesis-Osteogenesis Coupling and Immunomodulatory CGRP@Nano MOF-Loaded CMCS/GelMA Hydrogel for Bone Regeneration

Journal of Materials Chemistry B, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

The management of bone defects poses a significant challenge for clinicians, necessitating effective strategies to regulate immune inflammation, angiogenesis, and osteogenic differentiation successful regeneration. In this study, we developed novel hydrogel composed carboxymethyl chitosan (CMCS) gelatin methacryloyl (GelMA) designed the sustained release bioactive component calcitonin gene-related peptide (CGRP) zeolitic imidazolate framework-8 (ZIF-8). CGRP was initially encapsulated within ZIF-8 subsequently integrated into CMCS/GelMA matrix. vitro evaluations revealed that exhibited exceptional biocompatibility antimicrobial properties, effectively promoting osteogenesis angiogenesis while modulating M2 macrophage polarization. Furthermore, in vivo assessments indicated CGRP@MOF/CG significantly regulated local microenvironment accelerated healing cranial rat models. This study provides valuable references design fabrication multifunctional materials enhancing

Язык: Английский

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A review of computational optimization of bone scaffold architecture: Methods, challenges, and perspectives

Progress in Biomedical Engineering, Год журнала: 2024, Номер 7(1), С. 012003 - 012003

Опубликована: Окт. 16, 2024

Abstract The design and optimization of bone scaffolds are critical for the success tissue engineering (BTE) applications. This review paper provides a comprehensive analysis computational methods scaffold architecture, focusing on balance between mechanical stability, biological compatibility, manufacturability. Finite element method (FEM), fluid dynamics (CFD), various algorithms discussed their roles in simulating refining designs. integration multiobjective topology has been highlighted developing that meet multifaceted requirements BTE. Challenges such as need consideration manufacturing constraints incorporation degradation regeneration models into process have identified. underscores potential advanced tools additive techniques evolving field BTE, aiming to improve patient outcomes regeneration. reliability current is examined, with suggestions incorporating non-deterministic approaches vivo validations enhance practical application optimized scaffolds. concludes call further research artificial intelligence-based advance optimization.

Язык: Английский

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Designer micro/nano-crumpled MXene multilayer coatings accelerate osteogenesis and regulate macrophage polarization

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 11, 2024

Effective tissue regeneration and immune responses are essential for the success of biomaterial implantation. Although interaction between synthetic materials biological systems is well-recognized, role surface topographical cues in regulating local osteoimmune microenvironment - specifically, their impact on host cells dynamic interactions remains underexplored. This study addresses this gap by investigating topography osteogenesis immunomodulation. We fabricated MXene/Hydroxyapatite (HAP)-coated surfaces with controlled 2.5D nano-, submicro-, micro-scale patterns using our custom bottom-up pattering method. These engineered were employed to assess behavior osteoblast precursor macrophage polarization. Our results demonstrate that MXene/HAP-coated microscale crumpled significantly influence osteogenic activity polarization: notably enhanced cell spreading, proliferation, differentiation, facilitated a shift macrophages towards an anti-inflammatory, pro-healing M2 phenotype. The observed indicate physical from topographies, combined chemical MXene/HAP coatings, synergistically create favorable microenvironment. presents first evidence employing MXene/HAP-multilayer coated finely concurrently facilitate immunomodulation improved implant-to-tissue integration. tunable topographic these coupled facile scalable fabrication process, make them widely applicable various biomedical purposes. highlight potential novel coatings improve vivo performance fate implants modulating response at material interface.

Язык: Английский

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Lithium-doped calcium silicate regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 11, 2024

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement enhance regeneration modulate immune microenvironment promote tissue repair. We synthesized LiCS ceramic powder performed comprehensive analyses its physicochemical properties, including phase composition, morphology, setting time, mechanical strength. The results demonstrated that incorporation lithium into significantly increased diametral tensile strength (DTS) facilitated hydroxyapatite formation compared with undoped silicate. In vitro assays revealed enhanced proliferation, adhesion, spread Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines increasing anti-inflammatory cytokines, promoting polarization macrophages towards M2 phenotype. presence Li also improved osteogenic differentiation WJMSCs, as evidenced elevated levels alkaline phosphatase osteocalcin expression. These findings underscore dual functional capabilities enhancing osteogenesis modulating environment, making it promising material for engineering

Язык: Английский

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Development of an amphiphilic PLA Sponge by Solvent-Induced Phase Inversion: Effect of a bacterial Cellulose-g-Poly(methylmethacrylate) additive

Cellulose, Год журнала: 2024, Номер 31(12), С. 7553 - 7567

Опубликована: Июль 11, 2024

Язык: Английский

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Molecular dynamics simulations of ion adsorption on biphasic calcium phosphate surfaces: Effects of ionic environment and surface morphology

Applied Surface Science, Год журнала: 2024, Номер unknown, С. 162262 - 162262

Опубликована: Дек. 1, 2024

Язык: Английский

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