Lithium-doped calcium silicate regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration DOI Creative Commons
Tsung-Li Lin, Yen-Hong Lin, Kun-Hao Chen

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 11, 2024

Abstract Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement enhance regeneration modulate immune microenvironment promote tissue repair. We synthesized LiCS ceramic powder performed comprehensive analyses its physicochemical properties, including phase composition, morphology, setting time, mechanical strength. The results demonstrated that incorporation lithium into significantly increased diametral tensile strength (DTS) facilitated hydroxyapatite formation compared with undoped silicate. In vitro assays revealed enhanced proliferation, adhesion, spread Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines increasing anti-inflammatory cytokines, promoting polarization macrophages towards M2 phenotype. presence Li also improved osteogenic differentiation WJMSCs, as evidenced elevated levels alkaline phosphatase osteocalcin expression. These findings underscore dual functional capabilities enhancing osteogenesis modulating environment, making it promising material for engineering

Language: Английский

Lithium-doped calcium silicate cement regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration DOI Creative Commons
Yen-Hong Lin, Cheng-Yu Chen, Kun-Hao Chen

et al.

Journal of Biological Engineering, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 6, 2025

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement enhance regeneration modulate immune microenvironment promote tissue repair. We synthesized LiCS ceramic powder performed comprehensive analyses its physicochemical properties, including phase composition, morphology, setting time, mechanical strength. The results demonstrated that incorporation lithium into significantly increased diametral tensile strength (DTS) facilitated hydroxyapatite formation compared with undoped silicate. In vitro assays revealed enhanced proliferation, adhesion, spread Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines increasing anti-inflammatory cytokines, promoting polarization macrophages towards M2 phenotype. presence Li also improved osteogenic differentiation WJMSCs, as evidenced elevated levels alkaline phosphatase osteocalcin expression. These findings underscore dual functional capabilities enhancing osteogenesis modulating environment, making it promising material for engineering

Language: Английский

Citations

1
Designer Micro-/Nanocrumpled MXene Multilayer Coatings Accelerate Osteogenesis and Regulate Macrophage Polarization DOI

Mohammad Asadi Tokmedash,

Jouha Min

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(17), P. 21415 - 21426

Published: March 6, 2024

Effective tissue regeneration and immune responses are essential for the success of biomaterial implantation. Although interaction between synthetic materials biological systems is well-recognized, role surface topographical cues in regulating local osteoimmune microenvironment─specifically, their impact on host cells, dynamic interactions─remains underexplored. This study addresses this gap by investigating topography osteogenesis immunomodulation. We fabricated MXene/hydroxyapatite (HAP)-coated surfaces with controlled 2.5D nano-, submicro-, microscale patterns using our custom bottom-up patterning method. These engineered were employed to assess behavior osteoblast precursor cells macrophage polarization. Our results demonstrate that MXene/HAP-coated crumpled significantly influence osteogenic activity polarization: these notably enhanced cell spreading, proliferation, differentiation facilitated a shift macrophages toward an anti-inflammatory, prohealing M2 phenotype. The observed indicate physical from topographies, combined chemical MXene/HAP coatings, synergistically create favorable microenvironment. presents first evidence employing MXene/HAP-multilayer coated finely concurrently facilitate immunomodulation improved implant-to-tissue integration. tunable topographic coatings coupled facile scalable fabrication process make them widely applicable various biomedical purposes. highlight potential multilayer improve vivo performance fate implants modulating response at material interface.

Language: Английский

Citations

5
Applications and Progress of 3D Printed Bioceramic Scaffolds in Bone Tissue Repair and Immune Regulation DOI

Yasi Chen,

Shaohao Quan,

Sirui Huang

et al.

Ceramics International, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

5
Advances in the application and research of biomaterials in promoting bone repair and regeneration through immune modulation DOI Creative Commons
Li Liu, Hao Chen, Xue Zhao

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 30, P. 101410 - 101410

Published: Dec. 16, 2024

Language: Английский

Citations

3
Angiogenesis-Osteogenesis Coupling and Immunomodulatory CGRP@Nano MOF-Loaded CMCS/GelMA Hydrogel for Bone Regeneration DOI
Rong Luo, Chen Zhao,

Man Zhe

et al.

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The management of bone defects poses a significant challenge for clinicians, necessitating effective strategies to regulate immune inflammation, angiogenesis, and osteogenic differentiation successful regeneration. In this study, we developed novel hydrogel composed carboxymethyl chitosan (CMCS) gelatin methacryloyl (GelMA) designed the sustained release bioactive component calcitonin gene-related peptide (CGRP) zeolitic imidazolate framework-8 (ZIF-8). CGRP was initially encapsulated within ZIF-8 subsequently integrated into CMCS/GelMA matrix. vitro evaluations revealed that exhibited exceptional biocompatibility antimicrobial properties, effectively promoting osteogenesis angiogenesis while modulating M2 macrophage polarization. Furthermore, in vivo assessments indicated CGRP@MOF/CG significantly regulated local microenvironment accelerated healing cranial rat models. This study provides valuable references design fabrication multifunctional materials enhancing

Language: Английский

Citations

0
A review of computational optimization of bone scaffold architecture: Methods, challenges, and perspectives DOI Creative Commons
Ali H. Foroughi,

Caleb Valeri,

Mir Jalil Razavi

et al.

Progress in Biomedical Engineering, Journal Year: 2024, Volume and Issue: 7(1), P. 012003 - 012003

Published: Oct. 16, 2024

Abstract The design and optimization of bone scaffolds are critical for the success tissue engineering (BTE) applications. This review paper provides a comprehensive analysis computational methods scaffold architecture, focusing on balance between mechanical stability, biological compatibility, manufacturability. Finite element method (FEM), fluid dynamics (CFD), various algorithms discussed their roles in simulating refining designs. integration multiobjective topology has been highlighted developing that meet multifaceted requirements BTE. Challenges such as need consideration manufacturing constraints incorporation degradation regeneration models into process have identified. underscores potential advanced tools additive techniques evolving field BTE, aiming to improve patient outcomes regeneration. reliability current is examined, with suggestions incorporating non-deterministic approaches vivo validations enhance practical application optimized scaffolds. concludes call further research artificial intelligence-based advance optimization.

Language: Английский

Citations

2
Molecular dynamics simulations of ion adsorption on biphasic calcium phosphate surfaces: Effects of ionic environment and surface morphology DOI
Qiao Zhang, Xin Wang, Dingguo Xu

et al.

Applied Surface Science, Journal Year: 2024, Volume and Issue: unknown, P. 162262 - 162262

Published: Dec. 1, 2024

Language: Английский

Citations

1
Development of an amphiphilic PLA Sponge by Solvent-Induced Phase Inversion: Effect of a bacterial Cellulose-g-Poly(methylmethacrylate) additive DOI
Weepol Pramualkijja, Rachadaporn Benchawattananon, Kawee Srikulkit

et al.

Cellulose, Journal Year: 2024, Volume and Issue: 31(12), P. 7553 - 7567

Published: July 11, 2024

Language: Английский

Citations

0
Designer micro/nano-crumpled MXene multilayer coatings accelerate osteogenesis and regulate macrophage polarization DOI Creative Commons

Mohammad Asadi Tokmedash,

Jouha Min

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 11, 2024

Effective tissue regeneration and immune responses are essential for the success of biomaterial implantation. Although interaction between synthetic materials biological systems is well-recognized, role surface topographical cues in regulating local osteoimmune microenvironment - specifically, their impact on host cells dynamic interactions remains underexplored. This study addresses this gap by investigating topography osteogenesis immunomodulation. We fabricated MXene/Hydroxyapatite (HAP)-coated surfaces with controlled 2.5D nano-, submicro-, micro-scale patterns using our custom bottom-up pattering method. These engineered were employed to assess behavior osteoblast precursor macrophage polarization. Our results demonstrate that MXene/HAP-coated microscale crumpled significantly influence osteogenic activity polarization: notably enhanced cell spreading, proliferation, differentiation, facilitated a shift macrophages towards an anti-inflammatory, pro-healing M2 phenotype. The observed indicate physical from topographies, combined chemical MXene/HAP coatings, synergistically create favorable microenvironment. presents first evidence employing MXene/HAP-multilayer coated finely concurrently facilitate immunomodulation improved implant-to-tissue integration. tunable topographic these coupled facile scalable fabrication process, make them widely applicable various biomedical purposes. highlight potential novel coatings improve vivo performance fate implants modulating response at material interface.

Language: Английский

Citations

0
Lithium-doped calcium silicate regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration DOI Creative Commons
Tsung-Li Lin, Yen-Hong Lin, Kun-Hao Chen

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 11, 2024

Abstract Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement enhance regeneration modulate immune microenvironment promote tissue repair. We synthesized LiCS ceramic powder performed comprehensive analyses its physicochemical properties, including phase composition, morphology, setting time, mechanical strength. The results demonstrated that incorporation lithium into significantly increased diametral tensile strength (DTS) facilitated hydroxyapatite formation compared with undoped silicate. In vitro assays revealed enhanced proliferation, adhesion, spread Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines increasing anti-inflammatory cytokines, promoting polarization macrophages towards M2 phenotype. presence Li also improved osteogenic differentiation WJMSCs, as evidenced elevated levels alkaline phosphatase osteocalcin expression. These findings underscore dual functional capabilities enhancing osteogenesis modulating environment, making it promising material for engineering

Language: Английский

Citations

0