Опубликована: Янв. 1, 2024
Язык: Английский
Lab on a Chip, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Centrifugal microfluidics, with its advantages of rapid and precise fluid control without the need for external pressure, is widely applied in point-of-care testing.
Язык: Английский
Процитировано
3
Journal of Materials Chemistry B, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Near-infrared fluorescence imaging is key in biological exploration. Short emission wavelengths of xanthene dyes limit their use. Structural modifications shift emissions to the NIR-I/II range, enhancing biomedical applications.
Язык: Английский
Процитировано
1
RSC Chemical Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
In solid tumours, cancer cells modify their metabolic processes to endure environments with nutrient and oxygen scarcity due inadequate blood flow. A thorough understanding of this adaptive mechanism, which requires reliable microscopic techniques, is crucial for developing effective treatments. the present study, we used multi-wavelength photothermal (PT) microscopy visualise cellular response glucose deprivation in living derived from cervical cancer. We found increased mitochondrial PT signal intensity under conditions, indicative a correlation between crista density intensity. Furthermore, revealed that activity autophagy-lysosome system can be evaluated by detecting substances accumulated lysosomes. Using method, confirmed ferritin denatured proteins endoplasmic reticulum were within The detectability these using at visible wavelengths indicated presence iron ions. This method does not require labeling molecules provides information detailed insights into responses associated adaptation cell metabolism stress conditions.
Язык: Английский
Процитировано
0
Sensors and Actuators B Chemical, Год журнала: 2025, Номер 433, С. 137519 - 137519
Опубликована: Фев. 28, 2025
Язык: Английский
Процитировано
0
Journal of Peptide Science, Год журнала: 2025, Номер 31(5)
Опубликована: Апрель 13, 2025
Self-labelling proteins like SNAP- and HaloTag have advanced imaging in life sciences by enabling live-cell labeling with fluorophore-conjugated substrates. However, the typical one-fluorophore-per-protein system limits signal intensity. To address this, we developed a strategy using ALFA-tag system, 13-amino acid peptide recognized bio-orthogonal fluorescently labelled nanobody, for amplification. We synthesized pentavalent ALFA5 used an azidolysine conjugation Cy5-modified or ligand through strain-promoted click chemistry. In vitro measurements on SDS-PAGE showed labelling, peptides covalently reacted their respective tag. HEK293 cells expressing HaloTag-mGluR2 fusion were labeled ALFA5-Cy5 substrates, confocal microscopy revealed significant enhancement far-red intensity upon nanobody addition, as quantified integrated density ratios. Comparisons between substrates superior performance latter, achieving better signal-to-noise signal-to-background ratios, well overall plasma membrane-localized regions. Our results demonstrate potential of ALFA-tag-based systems to amplify SLP fluorescent signals. This combines photostability synthetic fluorophores multivalent labeling, providing powerful tool applications including super-resolution cells. Its versatility is expandable across diverse protein colors.
Язык: Английский
Процитировано
0
Organic & Biomolecular Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
This study examined the kinetics of phenylsulfonyl pyridines to free thiol, with reactivity that can be tuned by an order magnitude 1–5. The pyridine derivatives provide a powerful tool for precise protein modifications under mild conditions.
Язык: Английский
Процитировано
0
Trends in Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Biosensors, Год журнала: 2025, Номер 15(5), С. 274 - 274
Опубликована: Апрель 29, 2025
Fluorescence-lifetime imaging microscopy (FLIM) is a powerful technique for highly multiplexed in live cells. In this work, we present genetically encoded FLIM multiplexing platform based on combination of fluorogen-activating protein FAST and red-shifted fluorogen N871b from the arylidene-imidazolone family. We showed that series mutants exhibit similar steady-state optical properties complex with but have different fluorescence lifetimes. The brightness binding strength pairs these variants allows them to be successfully used up three intracellular structures living cells simultaneously.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0