An Activity-Based Near-Infrared (Nir) Fluorogenic Probe for Labeling Lysosomal Β-Hexosaminidase in Cells DOI
Injae Shin, Yujun Kim, Changhee Lee

et al.

Published: Jan. 1, 2024

Language: Английский

Recent advances in centrifugal microfluidics for point-of-care testing DOI
Huijuan Yuan,

Zeyu Miao,

Chao Wan

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Centrifugal microfluidics, with its advantages of rapid and precise fluid control without the need for external pressure, is widely applied in point-of-care testing.

Language: Английский

Citations

3
Xanthene-Based NIR organic phototheranostics agents: Design strategies and biomedical applications DOI
Xiao‐Yun Ran, Yuanfeng Wei, Yanling Wu

et al.

Journal of Materials Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Near-infrared fluorescence imaging is key in biological exploration. Short emission wavelengths of xanthene dyes limit their use. Structural modifications shift emissions to the NIR-I/II range, enhancing biomedical applications.

Language: Английский

Citations

1
Photothermal imaging of cellular responses to glucose deprivation DOI Creative Commons
Jun Miyazaki,

Ryotaro Wagatsuma,

Koji Okamoto

et al.

RSC Chemical Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

In solid tumours, cancer cells modify their metabolic processes to endure environments with nutrient and oxygen scarcity due inadequate blood flow. A thorough understanding of this adaptive mechanism, which requires reliable microscopic techniques, is crucial for developing effective treatments. the present study, we used multi-wavelength photothermal (PT) microscopy visualise cellular response glucose deprivation in living derived from cervical cancer. We found increased mitochondrial PT signal intensity under conditions, indicative a correlation between crista density intensity. Furthermore, revealed that activity autophagy-lysosome system can be evaluated by detecting substances accumulated lysosomes. Using method, confirmed ferritin denatured proteins endoplasmic reticulum were within The detectability these using at visible wavelengths indicated presence iron ions. This method does not require labeling molecules provides information detailed insights into responses associated adaptation cell metabolism stress conditions.

Language: Английский

Citations

0
An activity-based near-infrared (NIR) fluorogenic probe for labeling lysosomal β-hexosaminidase in cells DOI Creative Commons

Y.I. Kim,

Changhee Lee, Joo Hee Choi

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 433, P. 137519 - 137519

Published: Feb. 28, 2025

Language: Английский

Citations

0
Penta‐ALFA‐Tagged Substrates for Self‐Labelling Tags Allow Signal Enhancement in Microscopy DOI Creative Commons
Souvik Ghosh,

Ramona Birke,

Ashwin Karthick Natarajan

et al.

Journal of Peptide Science, Journal Year: 2025, Volume and Issue: 31(5)

Published: April 13, 2025

Self-labelling proteins like SNAP- and HaloTag have advanced imaging in life sciences by enabling live-cell labeling with fluorophore-conjugated substrates. However, the typical one-fluorophore-per-protein system limits signal intensity. To address this, we developed a strategy using ALFA-tag system, 13-amino acid peptide recognized bio-orthogonal fluorescently labelled nanobody, for amplification. We synthesized pentavalent ALFA5 used an azidolysine conjugation Cy5-modified or ligand through strain-promoted click chemistry. In vitro measurements on SDS-PAGE showed labelling, peptides covalently reacted their respective tag. HEK293 cells expressing HaloTag-mGluR2 fusion were labeled ALFA5-Cy5 substrates, confocal microscopy revealed significant enhancement far-red intensity upon nanobody addition, as quantified integrated density ratios. Comparisons between substrates superior performance latter, achieving better signal-to-noise signal-to-background ratios, well overall plasma membrane-localized regions. Our results demonstrate potential of ALFA-tag-based systems to amplify SLP fluorescent signals. This combines photostability synthetic fluorophores multivalent labeling, providing powerful tool applications including super-resolution cells. Its versatility is expandable across diverse protein colors.

Language: Английский

Citations

0
A systematic kinetic assay of phenylsulfonyl pyridine derivatives with free thiol for site-specific modification of proteins DOI

Xing Zhang,

Mohan Li,

Ben‐Guang Chen

et al.

Organic & Biomolecular Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This study examined the kinetics of phenylsulfonyl pyridines to free thiol, with reactivity that can be tuned by an order magnitude 1–5. The pyridine derivatives provide a powerful tool for precise protein modifications under mild conditions.

Language: Английский

Citations

0
Site-selective modification of native proteins DOI
Yujun Kim, Han Yi, Kyungdeok Seo

et al.

Trends in Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Fluorescence Lifetime Multiplexing with Fluorogen-Activating FAST Protein Variants and Red-Shifted Arylidene–Imidazolone Derivative as Fluorogen DOI Creative Commons
Aidar R. Gilvanov, Ivan N. Myasnyanko, Sergey A. Goncharuk

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 274 - 274

Published: April 29, 2025

Fluorescence-lifetime imaging microscopy (FLIM) is a powerful technique for highly multiplexed in live cells. In this work, we present genetically encoded FLIM multiplexing platform based on combination of fluorogen-activating protein FAST and red-shifted fluorogen N871b from the arylidene–imidazolone family. We showed that series mutants exhibit similar steady-state optical properties complex with but have different fluorescence lifetimes. The brightness binding strength pairs these variants allows them to be successfully used up three intracellular structures living cells simultaneously.

Language: Английский

Citations

0
An Activity-Based Near-Infrared (Nir) Fluorogenic Probe for Labeling Lysosomal Β-Hexosaminidase in Cells DOI
Injae Shin, Yujun Kim, Changhee Lee

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0