Environment International, Год журнала: 2024, Номер 194, С. 109128 - 109128
Опубликована: Ноя. 14, 2024
Estimating effective reproduction number (R
Язык: Английский
Environmental Science & Technology Letters, Год журнала: 2024, Номер 11(6), С. 526 - 532
Опубликована: Май 20, 2024
Prospective influenza A (IAV) RNA monitoring at 190 wastewater treatment plants (WWTPs) across the US identified increases in IAV concentrations 59 spring 2024, after typical seasonal period, coincident with identification of highly pathogenic avian (subtype H5N1) circulating dairy cattle US. We developed and validated a hydrolysis-probe RT-PCR assay for quantification H5 hemagglutinin gene. applied it retrospectively to samples from four WWTPs where springtime were one WWTP they not. The marker was detected all coinciding not without an increase. Positive are located states confirmed outbreaks influenza, H5N1 clade 2.3.4.4b, cattle. Concentrations gene approached overall virus concentrations, suggesting large fraction inputs subtypes. At positive WWTPs, industrial discharges containing animal waste, including milk byproducts, permitted discharge into sewers. Our findings demonstrate that can detect animal-associated contributions highlight need consider agricultural wastewater. This work illustrates monitoring's value comprehensive surveillance, influenzas currently thought be primarily found animals important implications human health.
Язык: Английский
Процитировано
26
medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Abstract In 2024, the highly pathogenic avian influenza A H5N1 caused outbreaks in wild birds, poultry, cows, and other mammals United States with 61 human cases also reported by CDC. Detection of H5 RNA wastewater has been previously sewersheds Texas North Carolina nearby impacted dairy herds following emergence cows. Here, we conduct retrospective testing total hemagglutinin genes as well presenting applying new assays for detection H1 H3 across a respiratory virus season an urban California sewershed from September 2023 – May 2024. Total A, H1, were regularly detected, while was first detected March. We developed model that uses Monte Carlo simulations published parameters to estimate numbers infected people, or liters H5-contaminated milk required result measured concentrations wastewater. Our findings demonstrate this sewershed, contaminated poultry most likely sources created publicly available tool apply input inputs. Synopsis understand potential wastewater, enabling interpretation detections. TOC figure
Язык: Английский
Процитировано
2
Infectious Medicine, Год журнала: 2025, Номер unknown, С. 100177 - 100177
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
2
Scientific Data, Год журнала: 2024, Номер 11(1)
Опубликована: Окт. 17, 2024
We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A B viruses, syncytial virus, human metapneumovirus, enterovirus D68, parainfluenza types 1, 2, 3, 4a, 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis adenovirus, mpox H5 pepper mild mottle virus nucleic acids wastewater solids prospectively at 191 treatment plants 40 states across the United States plus Washington DC. Measurements were made two to seven times per week from 1 January 2022 30 June 2024, depending on plant staff availability. using droplet digital (reverse-transcription-) polymerase chain reaction (ddRT-PCR) following best practices for making environmental molecular biology measurements. These data can be used better understand disease occurrence communities contributing wastewater.
Язык: Английский
Процитировано
6
The Science of The Total Environment, Год журнала: 2024, Номер 951, С. 175473 - 175473
Опубликована: Авг. 12, 2024
Hepatitis A and E viruses (HAV HEV, respectively) remain a significant global health concern despite advancements in healthcare vaccination programs. Regular monitoring vaccine efficacy of HAV are still lacking different countries. This study aimed to investigate HEV prevalence developed, developing, least-developed Asian countries using wastewater as surveillance tool. total 232 untreated samples were collected from six treatment plants, sewage plant, or an open drainage [Nepal (n = 51), Indonesia 37), Thailand 30), Vietnam 27), the Philippines 17), Japan 70)] between April October 2022. Viruses concentrated by simple centrifugation polyethylene glycol precipitation method, followed viral RNA extraction reverse transcription-quantitative polymerase chain reaction. detected Nepal (51 % for 2 HEV), (3 both viruses), (1 24 HEV). Only was found 11 Indonesia, whereas only Philippines, with positive ratio 15 12 %, respectively. These results highlighted geographic variability prevalence, underscoring need localized public strategies address specific hepatitis challenges each country.
Язык: Английский
Процитировано
4
Environmental Science & Technology, Год журнала: 2024, Номер unknown
Опубликована: Дек. 9, 2024
Wastewater surveillance of vaccine-preventable diseases may provide early warning outbreaks and identify areas to target for immunization. To advance wastewater monitoring measles, mumps, rubella viruses, we developed validated a multiplexed RT-ddPCR assay the detection their RNA. Because measles-mumps-rubella (MMR) vaccine is an attenuated live virus vaccine, also that distinguishes between wild-type strains measles in it using sample collected from facility with active outbreak. We evaluated partitioning behavior viruses liquid solid fractions influent wastewater. found assaying fraction resulted more sensitive despite fact viral RNA was enriched due low solids content Finally, investigated stability samples spiked over 28 days at two different concentrations temperatures (4 °C room temperature) through freeze-thaw observed limited decay. Our study supports feasibility population-level surveillance.
Язык: Английский
Процитировано
4
Environmental Science & Technology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 11, 2025
This study proposes a novel approach for viral infectious disease surveillance using Google Trends data to model wastewater virus concentrations, providing rapid, low-cost method indicating outbreaks. search terms were found correlate strongly with concentrations and clinical cases influenza A respiratory syncytial (R2 = 0.76 0.66). For norovirus mpox, which limited, showed significant correlations concentrations. Three modeling approaches developed: simple linear, stepwise selection, principal component analysis. These models demonstrated strong predictive power both of up 0.66) mpox 0.60) The was validated case documented 2021 outbreak in Hartford, CT, where indicators rose tandem potentially earlier detection than data. offers complementary stream public health decision-making, particularly valuable areas lacking robust testing infrastructure. Limitations include potential confounding factors, such as media coverage the need consider local idioms international applications.
Язык: Английский
Процитировано
0
Water Research, Год журнала: 2025, Номер 283, С. 123857 - 123857
Опубликована: Май 17, 2025
Язык: Английский
Процитировано
0
Water Research, Год журнала: 2025, Номер 284, С. 123904 - 123904
Опубликована: Май 28, 2025
Язык: Английский
Процитировано
0
The Science of The Total Environment, Год журнала: 2025, Номер 986, С. 179706 - 179706
Опубликована: Май 30, 2025
Язык: Английский
Процитировано
0