Biosensors and Bioelectronics, Год журнала: 2024, Номер 271, С. 117084 - 117084
Опубликована: Дек. 24, 2024
Язык: Английский
Physics of Fluids, Год журнала: 2025, Номер 37(2)
Опубликована: Фев. 1, 2025
The challenges of constraints and imprecision in chemical analysis medical diagnosis are particularly evident the separation blood cells. Pinched flow fractionation, a passive microfluidic technique, has gained attention for its potential size-based cell separation. Enhancing efficiency is crucial, especially through integration with external forces or active methods. This study explores use dielectrophoresis (DEP) magnetophoresis (MP) to separate platelet cells, red white circulating tumor These were analyzed both independently simultaneously. To achieve optimal separation, Navier–Stokes equations, Newton's second law, system's electric magnetic fields modeled. Experiments conducted branched microchannel assess impact various parameters, including buffer rate, pinched section width, applied voltage, field frequency, intensity. revealed approximately 99% under conditions Wp=20 μm, Q2=1250 μl/h, f=100 kHz, V=3 V, M=1.5 T. findings demonstrate that while DEP MP individually enhance their simultaneous application significantly improves precision, achieving complete optimized system holds great promise applications analytical chemistry diagnostics, analysis.
Язык: Английский
Процитировано
2
Lab on a Chip, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Rapid and accurate molecular diagnostics are crucial for preventing the global spread of emerging infectious diseases. However, current gold standard nucleic acid detection, reverse transcription polymerase chain reaction (RT-PCR), relies heavily on traditional magnetic beads or silica membranes extraction, resulting in several limitations, including time-consuming processes, need trained personnel, complex equipment. Therefore, there is an urgent fully integrated detection technologies that simple to operate, rapid, highly sensitive meet unmet clinical needs. In this study, we developed a novel, microfluidic cartridge featuring unique needle-plug/piston microvalve, which enables stable long-term reagent storage flexible liquid handling on-site analysis. Coupled with
Язык: Английский
Процитировано
1
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Янв. 15, 2025
To facilitate on-site detection by nonspecialists, there is a demand for the development of portable "sample-to-answer" devices capable executing all procedures in an automated or easy-to-operate manner. Here, we developed device that integrated magnetofluidic manipulation system and signal acquisition system. Both systems were controllable via smartphone. In device, mixing solutions magnetic beads static chamber was enhanced steel bead agitation, which improved reaction efficiency. We demonstrate performance using myoglobin as example. During process, plasma separated from whole blood sample homemade mini-centrifuge, subsequently, plasma, beads, reagents added to chip with multiple chambers. After loaded, initiated smartphone App Bluetooth. Then, shuttled through different chambers steps completed automatically: first, targets enriched antibody-modified followed washing, binding aptamer-functionalized G-quadruplex, amplifying (optional), chromogenic reaction. Finally, images colored captured processed obtain concentrations myoglobin. The limits depended on mode conversion, 0.1 2.7 nM (with without amplifying). With its simple operation, compact design, low cost, ease scalability, this holds potential applications human health, food safety, environmental monitoring, etc.
Язык: Английский
Процитировано
1
Lab on a Chip, Год журнала: 2024, Номер 24(18), С. 4275 - 4287
Опубликована: Янв. 1, 2024
Rapid and sensitive detection of pathogens in various samples is crucial for disease diagnosis, environmental surveillance, as well food water safety monitoring. However, the low abundance (<10 CFU) large volume (1 mL-1 L) containing vast backgrounds critically limits sensitivity even most advanced techniques, such digital PCR. Therefore, there a critical need sample preparation that can enrich low-abundance from complex large-volume samples. This study develops an efficient electrostatic microfiltration (EM)-based technique capable processing ultra-large-volume (≥500 mL) at high throughput (≥10 mL min
Язык: Английский
Процитировано
3
Chemical Engineering and Processing - Process Intensification, Год журнала: 2025, Номер unknown, С. 110153 - 110153
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Lab on a Chip, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Created in BioRender. Menon, N. (2025). https://www.BioRender.com/l48m487.
Язык: Английский
Процитировано
0
Diagnostic Microbiology and Infectious Disease, Год журнала: 2025, Номер unknown, С. 116716 - 116716
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
ACS Sensors, Год журнала: 2025, Номер unknown
Опубликована: Фев. 10, 2025
Bacterial infections often lead to severe health consequences owing their ability infiltrate multiple anatomical sites, including the bloodstream, respiratory tract, and digestive posing substantial diagnostic therapeutic challenges. Consequently, a rapid versatile detection method capable of identifying broad spectrum bacterial pathogens is urgently required facilitate precise antibiotic prescriptions. Addressing this need, we introduce MiND-DMF (Multibacterial Infection Nucleic Acid Detection on Digital Microfluidic Platform), cost-effective digital microfluidic platform tailored for multiplexed detection. This system integrates DNA extraction, recombinase polymerase amplification (RPA), CRISPR-based technologies, enabling efficient identification six common infectious bacteria. Operating at constant temperature 37 °C, completes entire process in just 55 min compatible with human reference genes. In spiked samples, demonstrated limit 100 CFU/mL, highlighting its exceptional sensitivity quantification capability. clinical evaluations, exhibited outstanding performance, achieving 100% 98%–100% specificity compared conventional PCR methods across 50 samples derived from diverse tissue sources. robust demonstrates strong anti-interference capabilities, making it suitable analyzing various fluids blood, alveolar lavage fluid, urine, nasal secretions, appendiceal pus, ear pus. The versatility precision support monitoring hospital-acquired infection origins, empowering physicians prescribe targeted antibiotics enhancing overall prevention control strategies. By accurately detecting bacteria sources, can play pivotal role improving patient outcomes public health.
Язык: Английский
Процитировано
0
iLABMED, Год журнала: 2025, Номер unknown
Опубликована: Март 10, 2025
ABSTRACT The combination of low‐consumption microfluidic chips and high‐sensitivity biosensors enables rapid accurate detection complex target analytes. This integrated system holds significant potential for applications in disease diagnosis, health monitoring, treatment management. Advances novel biomaterials have led to device integration into wearable implantable systems point‐of‐care testing. Here, we review recent advances clinical detecting nucleic acids, proteins, metabolites, pathogens, cellular components. We outline the prospects devices based on analysis biofluids such as sweat discuss remaining challenges facing application biosensors.
Язык: Английский
Процитировано
0
Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер 252, С. 114670 - 114670
Опубликована: Апрель 6, 2025
Язык: Английский
Процитировано
0