Synergistic effects of dielectrophoretic and magnetophoretic forces on continuous cell separation via pinched flow fractionation

Physics of Fluids, Journal Year: 2025, Volume and Issue: 37(2)

Published: Feb. 1, 2025

The challenges of constraints and imprecision in chemical analysis medical diagnosis are particularly evident the separation blood cells. Pinched flow fractionation, a passive microfluidic technique, has gained attention for its potential size-based cell separation. Enhancing efficiency is crucial, especially through integration with external forces or active methods. This study explores use dielectrophoresis (DEP) magnetophoresis (MP) to separate platelet cells, red white circulating tumor These were analyzed both independently simultaneously. To achieve optimal separation, Navier–Stokes equations, Newton's second law, system's electric magnetic fields modeled. Experiments conducted branched microchannel assess impact various parameters, including buffer rate, pinched section width, applied voltage, field frequency, intensity. revealed approximately 99% under conditions Wp=20 μm, Q2=1250 μl/h, f=100 kHz, V=3 V, M=1.5 T. findings demonstrate that while DEP MP individually enhance their simultaneous application significantly improves precision, achieving complete optimized system holds great promise applications analytical chemistry diagnostics, analysis.

Language: Английский

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A fully integrated microfluidic cartridge for rapid and ultrasensitive nucleic acid detection from oropharyngeal swabs

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Rapid and accurate molecular diagnostics are crucial for preventing the global spread of emerging infectious diseases. However, current gold standard nucleic acid detection, reverse transcription polymerase chain reaction (RT-PCR), relies heavily on traditional magnetic beads or silica membranes extraction, resulting in several limitations, including time-consuming processes, need trained personnel, complex equipment. Therefore, there is an urgent fully integrated detection technologies that simple to operate, rapid, highly sensitive meet unmet clinical needs. In this study, we developed a novel, microfluidic cartridge featuring unique needle-plug/piston microvalve, which enables stable long-term reagent storage flexible liquid handling on-site analysis. Coupled with

Language: Английский

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Magnetofluidic-Assisted Portable Automated Microfluidic Devices for Protein Detection

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

To facilitate on-site detection by nonspecialists, there is a demand for the development of portable "sample-to-answer" devices capable executing all procedures in an automated or easy-to-operate manner. Here, we developed device that integrated magnetofluidic manipulation system and signal acquisition system. Both systems were controllable via smartphone. In device, mixing solutions magnetic beads static chamber was enhanced steel bead agitation, which improved reaction efficiency. We demonstrate performance using myoglobin as example. During process, plasma separated from whole blood sample homemade mini-centrifuge, subsequently, plasma, beads, reagents added to chip with multiple chambers. After loaded, initiated smartphone App Bluetooth. Then, shuttled through different chambers steps completed automatically: first, targets enriched antibody-modified followed washing, binding aptamer-functionalized G-quadruplex, amplifying (optional), chromogenic reaction. Finally, images colored captured processed obtain concentrations myoglobin. The limits depended on mode conversion, 0.1 2.7 nM (with without amplifying). With its simple operation, compact design, low cost, ease scalability, this holds potential applications human health, food safety, environmental monitoring, etc.

Language: Английский

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Hydrodynamic and mixing characteristics in oscillating feedback micromixers: influence of geometric configuration and scale

Chemical Engineering and Processing - Process Intensification, Journal Year: 2025, Volume and Issue: unknown, P. 110153 - 110153

Published: Jan. 1, 2025

Language: Английский

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Microfluidics for morpholomics and spatial omics applications

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Created in BioRender. Menon, N. (2025). https://www.BioRender.com/l48m487.

Language: Английский

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Strengthening Molecular Testing Capacity in Colombia: Challenges and Opportunities

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: unknown, P. 116716 - 116716

Published: Jan. 1, 2025

Language: Английский

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Rapid Bacterial Identification through Multiplexed Nucleic Acid Detection on a Digital Microfluidic Platform for Enhanced Clinical Intervention against Infections

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Bacterial infections often lead to severe health consequences owing their ability infiltrate multiple anatomical sites, including the bloodstream, respiratory tract, and digestive posing substantial diagnostic therapeutic challenges. Consequently, a rapid versatile detection method capable of identifying broad spectrum bacterial pathogens is urgently required facilitate precise antibiotic prescriptions. Addressing this need, we introduce MiND-DMF (Multibacterial Infection Nucleic Acid Detection on Digital Microfluidic Platform), cost-effective digital microfluidic platform tailored for multiplexed detection. This system integrates DNA extraction, recombinase polymerase amplification (RPA), CRISPR-based technologies, enabling efficient identification six common infectious bacteria. Operating at constant temperature 37 °C, completes entire process in just 55 min compatible with human reference genes. In spiked samples, demonstrated limit 100 CFU/mL, highlighting its exceptional sensitivity quantification capability. clinical evaluations, exhibited outstanding performance, achieving 100% 98%–100% specificity compared conventional PCR methods across 50 samples derived from diverse tissue sources. robust demonstrates strong anti-interference capabilities, making it suitable analyzing various fluids blood, alveolar lavage fluid, urine, nasal secretions, appendiceal pus, ear pus. The versatility precision support monitoring hospital-acquired infection origins, empowering physicians prescribe targeted antibiotics enhancing overall prevention control strategies. By accurately detecting bacteria sources, can play pivotal role improving patient outcomes public health.

Language: Английский

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Recent Advancements in Microfluidic Biosensors for Clinical Applications

iLABMED, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

ABSTRACT The combination of low‐consumption microfluidic chips and high‐sensitivity biosensors enables rapid accurate detection complex target analytes. This integrated system holds significant potential for applications in disease diagnosis, health monitoring, treatment management. Advances novel biomaterials have led to device integration into wearable implantable systems point‐of‐care testing. Here, we review recent advances clinical detecting nucleic acids, proteins, metabolites, pathogens, cellular components. We outline the prospects devices based on analysis biofluids such as sweat discuss remaining challenges facing application biosensors.

Language: Английский

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Metal-organic frameworks-molecularly imprinted polymers (MOF-MIP): Synthesis, properties, and applications in detection and control of microorganisms

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 252, P. 114670 - 114670

Published: April 6, 2025

Language: Английский

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Handheld RPA-Based Molecular POCT System for Rapid, Low-Cost 8-Plexed Detection of Respiratory Pathogens at Home

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Introducing a handheld microfluidic RPA POCT system for home use, enabling extraction-free, 8-plex respiratory pathogen detection in <25 min at just $1.4 per test.

Language: Английский

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