Опубликована: Янв. 1, 2024
Язык: Английский
Molecular Aspects of Medicine, Год журнала: 2025, Номер 102, С. 101350 - 101350
Опубликована: Фев. 10, 2025
Язык: Английский
Процитировано
1
Small Science, Год журнала: 2024, Номер unknown
Опубликована: Июль 25, 2024
The genetic material within cells plays a pivotal role in shaping the structure and function of living organisms. Manipulating an organism's genome to correct inherited abnormalities or introduce new traits holds great promise. Genetic engineering techniques offers promising pathways for precisely altering cellular genetics. Among these methodologies, clustered regularly interspaced short palindromic repeat (CRISPR), honored with 2020 Nobel Prize Chemistry, has garnered significant attention its precision editing genomes. However, CRISPR system faces challenges when applied vivo, including low delivery efficiency, off-target effects, instability. To address challenges, innovative technologies targeted precise have emerged. Engineered carrier platforms represent substantial advancement, improving stability, precision, reducing side effects associated editing. These facilitate efficient local systemic various tissues cells, immune cells. This review explores recent advances, benefits, CRISPR-based delivery. It examines carriers nanocarriers (polymeric, lipid-derived, metallic, bionanoparticles), viral particles, virus-like exosomes, providing insights into their clinical utility future prospects.
Язык: Английский
Процитировано
5
MedComm, Год журнала: 2025, Номер 6(5)
Опубликована: Май 1, 2025
ABSTRACT Single‐stranded DNA or RNA entities referred to as aptamers exhibit a strong affinity and specificity for attaching specific targets. Owing their special properties, which include simplicity of synthesis, low immunogenicity, adaptability in targeting variety substances, these synthetic oligonucleotides have garnered lot interest. The function can be altered by combining them with complementary “antidotes,” are antisense particular aptamer sequence. Antidotes play an important role several fields specifically the corresponding section aptamer. Nevertheless, even promising capabilities, creation antidotes regulate inhibit continues relatively unexamined field, constraining secure efficient application medical environments. review explores experimental methodologies creating antidotes, systematic design strategies managing aptamer‐based therapies, therapeutic efficacy counteracting disease biomarkers. Additionally, it highlights diagnostic applications biosensing imaging, offering alternative traditional antibodies. It also investigates progress, latest innovations, potential uses aptamer–antidote combinations. Its academic value lies bridging gap between theoretical practical applications, providing researchers clinicians comprehensive resource advance solutions medicine biotechnology.
Язык: Английский
Процитировано
0
Precision medicine and engineering., Год журнала: 2025, Номер unknown, С. 100031 - 100031
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
Bioconjugate Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Май 22, 2025
Targeted delivery of cytostatic drugs is a powerful approach to achieving tumor tissue selectivity, reducing systemic toxicity, and ultimately improving the efficacy anticancer chemotherapy. Targeting can be achieved using wide range molecular ligands, with DNA aptamers being promising representative. In this work, we employed flow cytometry, AuNP-aptasensor, atomic-scale computer modeling assess affinity several (Anti-HER2, HB5, Apt-6, HeA2_1, HeA2_3) for human epidermal growth factor receptor 2 (HER2), which known one factors that promote breast cancer cells. Flow cytometry showed short (HeA2_1 had higher HER2 on MDAMB453 cells than longer (HB5, Apt-6). HER2-negative MDA-MB-231 served as negative control. The HeA2_3 aptamer has high average (HeA2_3:23.6, HeA2_1:13.1, Apt-6:3.6; HB5:3.5; Anti-HER2:3.2) nearly Gaussian distribution across cells, while HeA2_1 forms fraction relatively fluorescence signal intensity (HeA2_1:11.6; HeA2_3:5.9; Apt-6:3.4; HB5:3.1; Anti-HER2:2.1). Most findings also hold HER2-positive small extracellular vesicles studied AuNP-aptasensor. Computer simulations confirmed are characterized by stronger binding domain HER2. A detailed analysis free energy allowed us show first time tight correlates well-separated hot cold spots protein surface. For meet these criteria (HeA2_1, HeA2_3, Anti-HER2), favorable interactions driven local attraction nucleotides arginine lysine residues possibly stabilized intermolecular hydrogen bonds. (Apt-6 HB5), surface overlap much weaker binding. Overall, our cannot merely dissociation equilibrium constant. more sophisticated combines experimental computational methods unlock mechanisms behind aptamer-HER2 bindings. results study suggest become reliable accurate tool prescreening prior laboratory experiments.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0