Multi-Organelle Imaging with Dye Combinations: Targeting the ER, Mitochondria, and Plasma Membrane DOI
Yogesh Dubey, Sriram Kanvah

Journal of Materials Chemistry B, Год журнала: 2024, Номер unknown

Опубликована: Дек. 30, 2024

Multi-organelle imaging allows the visualization of multiple organelles within a single cell, allowing monitoring cellular processes in real-time using various fluorescent probes that target specific organelles. However, limited availability fluorophores and potential spectral overlap present challenges, many optimized designs are still nascency. In this work, we synthesized sulfonamide-based organic emit blue, green, red regions to different sub-cellular By utilizing binary mixtures, successfully demonstrated organelles, such as endoplasmic reticulum, plasma membrane, mitochondria HeLa cells, dual reticulum A549 lung carcinoma cells with help blue red-emitting without any spillover. Additionally, these photostable allowed precise cell staining differentiation, structural features, live dynamics. This approach mixtures can gain traction for studies investigations.

Язык: Английский

A BODIPY-Naphtholimine-BF2 Dyad for Precision Photodynamic Therapy, Targeting, and Dual Imaging of Endoplasmic Reticulum and Lipid Droplets in Cancer DOI Creative Commons
Nitish Chauhan, Mrunesh Koli, Rajib Ghosh

и другие.

JACS Au, Год журнала: 2024, Номер 4(8), С. 2838 - 2852

Опубликована: Июнь 5, 2024

Currently, effective therapeutic modalities for pancreatic ductal adenocarcinoma (PDAC) are quite limited, leading to gloomy prognosis and ∼6-months median patient survival. Recent advances showed the promise of photodynamic therapy (PDT) PDAC patients. Next generation photosensitizers (PS) based on "organelle-targeted-PDT" provide new paradigm in field precision medicines address current challenge treating PDAC. In this investigation, we have constructed a novel PS, named as

Язык: Английский

Процитировано

6

Amino‐Terephthalonitrile Based Single Benzene Fluorophores Bearing Amino Esters and Lipids for Selective Biothiol Sensing and Bioimaging DOI Open Access
Ankita Sinha, Subhadeep Banerjee, Arka Bagchi

и другие.

Asian Journal of Organic Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Abstract We report here the preparation of eight new AmTN SBFs which feature amino esters with an N‐terminal fluorophore and also lipophilic dyes, consisting a push‐pull moiety. Spectrofluorimetric NMR titration investigations reveal preference terephthalonitirle ring in mono AmTNs towards rapid sensing N‐acetyl cysteine, as opposed to dipeptide carnosine. This has significance field bioanalysis whereby could show their potential thiol platforms. Also, one green emissive featuring two cyclohexyl units proved its worth fluorescence imaging using C2 C12 cells, where it localized electively inside cell cytoplasm at concentration 1 μM.

Язык: Английский

Процитировано

0

A novel FRET-based fluorescent probe capable of simultaneously imaging lipid droplets and the endoplasmic reticulum with two distinct fluorescence signals in HeLa cells DOI
Tianzhen Liu,

Xiao-Hui Chi,

Bing-Yu Wei

и другие.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер unknown, С. 126262 - 126262

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Fluorescence Lifetime Imaging: A Novel Approach to Simultaneous Visualizing Lipid Droplets and Endoplasmic Reticulum Polarity in Live Cells and Fatty Liver DOI
Zixuan Zhan,

Ridong Huang,

Li Chai

и другие.

Sensors and Actuators B Chemical, Год журнала: 2024, Номер unknown, С. 136699 - 136699

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

1

A novel fluorescent probe for viscosity and polarity detection in real tobacco root cells and biological imaging DOI
Yuan He,

Long-Ke Li,

Maohua Wang

и другие.

Photochemical & Photobiological Sciences, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

0

Multi-Organelle Imaging with Dye Combinations: Targeting the ER, Mitochondria, and Plasma Membrane DOI
Yogesh Dubey, Sriram Kanvah

Journal of Materials Chemistry B, Год журнала: 2024, Номер unknown

Опубликована: Дек. 30, 2024

Multi-organelle imaging allows the visualization of multiple organelles within a single cell, allowing monitoring cellular processes in real-time using various fluorescent probes that target specific organelles. However, limited availability fluorophores and potential spectral overlap present challenges, many optimized designs are still nascency. In this work, we synthesized sulfonamide-based organic emit blue, green, red regions to different sub-cellular By utilizing binary mixtures, successfully demonstrated organelles, such as endoplasmic reticulum, plasma membrane, mitochondria HeLa cells, dual reticulum A549 lung carcinoma cells with help blue red-emitting without any spillover. Additionally, these photostable allowed precise cell staining differentiation, structural features, live dynamics. This approach mixtures can gain traction for studies investigations.

Язык: Английский

Процитировано

0