Silk Sericin/Chitosan Supramolecular Multilayered Thin Films as Sustainable Cytocompatible Nanobiomaterials DOI

Miguel Padilla Rosas,

Cristiana F. V. Sousa,

Ana Pereira

и другие.

Biomacromolecules, Год журнала: 2024, Номер 26(1), С. 296 - 310

Опубликована: Дек. 16, 2024

Silk sericin (SS) has been widely discarded as a waste by the silk textile industry during degumming process to obtain fibroin. However, in past decade, an in-depth understanding of its properties and functions turned it into high added-value biomaterial for biomedical applications. Herein, we report molecular design development sustainable supramolecular multilayered nanobiomaterials encompassing SS oppositely charged chitosan (CHT) through combination self-assembly electrostatically driven layer-by-layer (LbL) assembly technology. The successful buildup SS/CHT was demonstrated quartz crystal microbalance with dissipation monitoring attenuated total reflectance-Fourier transform infrared spectroscopy, nanofilms' wettable nanofibrillar-like topography were shown water contact angle, atomic force microscopy, scanning electron microscopy. In vitro assays cytocompatibility LbL nanofilms toward human primary dermal fibroblasts, holding great promise biofunctional nanocoatings drug/therapeutics/cell delivery, tissue engineering, regenerative medicine.

Язык: Английский

Biowaste Transformation to Functional Materials: Structural Properties, Extraction Methods, Applications, and Challenges of Silk Sericin DOI Creative Commons

Mohashin Kabir,

Mohammed Kamrul Hasan,

Mohamad Rafi

и другие.

ChemistrySelect, Год журнала: 2024, Номер 9(42)

Опубликована: Ноя. 1, 2024

Abstract This review underscores the novelty of silk sericin, often regarded as a byproduct in production. Comprising approximately 30% silk, sericin possesses valuable properties that have been largely overlooked favor fibroin. work focuses on innovative extraction methods to convert typically considered waste, into resource. By examining these techniques alongside diverse applications, aims enhance recognition and utilization research industry. Various processes where traditional methods, some new techniques, are all discussed. In detail, advantages, limitations, strategy optimization associated with each method highlighted. For instance, has shown promise wound treatment agent, scaffold for tissue engineering, carrier drug delivery, biomaterial regenerative medicine field biomedical science due its biocompatibility, biodegradability, nontoxicity. Additionally, it also finds application cosmetic industry, is used skincare products moisturizing, antioxidant, antiaging properties. Eventually, challenges, prospects intensively comprehensive expected serve growing body knowledge surrounding foster further development sericin‐related fields.

Язык: Английский

Процитировано

4

Innovative Processing and Sterilization Techniques to Unlock the Potential of Silk Sericin for Biomedical Applications DOI Creative Commons
Anabela Veiga, Rosa Ana Ramírez-Jiménez, Víctor Santos‐Rosales

и другие.

Gels, Год журнала: 2025, Номер 11(2), С. 114 - 114

Опубликована: Фев. 6, 2025

Silk sericin (SS), a by-product of the textile industry, has gained significant attention for its biomedical potential due to biocompatibility and regenerative potential. However, literature lacks information on SS processing methods resulting physicochemical properties. This study represents first step in protocol optimization standardization. In present work, different techniques were studied compared extracted from boiling water: evaporation, rotary lyophilization, dialysis, which presented recovery yield approximately 27–32%. The goal was find most promising process concentrate solutions, ensure that structure highly preserved. As result, new cryo-lyophilization methodology proposed. proposed method allows preservation amorphous structure, offers advantages including complete dissolution water PBS, an increase storage stability, possibility scaling-up, making it suitable industrial applications. second part work focused addressing another challenge processing: efficient non-destructive sterilization. Supercritical CO2 (scCO2) been gaining momentum last years sterilizing sensitive biopolymers biological materials non-toxicity mild conditions. Thus, scCO2 technology validated as technique terminal sterilization SS. this way, possible engineer sequential cryo-lyophilization/scCO2 able preserve original properties natural silk protein. Overall, we have valorized into sterile, off-the-shelf, bioactive, water-soluble material, with be used biomedical, pharmaceutical, or cosmetic industries.

Язык: Английский

Процитировано

0

Native Silk Fibers: Protein Sequence and Structure Influences on Thermal and Mechanical Properties DOI
Elizabeth Aikman, Lauren E. Eccles, Whitney L. Stoppel

и другие.

Biomacromolecules, Год журнала: 2025, Номер unknown

Опубликована: Март 7, 2025

Silk fibers produced by arthropods have inspired an array of materials with applications in healthcare, medical devices, textiles, and sustainability. Silks exhibit biodiversity distinct variations primary protein constituent sequences (fibroins, spidroins) structures across taxonomic classifications, specifically the Lepidopteran Araneae orders. Leveraging arthropod silks offers advantages due to diverse mechanical properties thermal stabilities achievable, primarily attributed fiber crystallinity repeating amino acid motifs. In this review, we aim delineate known silk correlate them predicted secondary structures, informed newly annotated genomes. We will discuss established patterns repeat motifs governing specific underscore biological diversity within fibroin spidroin sequences. Elucidating relationship between natural identify strategies for designing new through rational silk-based design.

Язык: Английский

Процитировано

0

Recent advancements and challenges in 3D bioprinting for cancer applications DOI
Swayam Aryam Behera, Binita Nanda, P. Ganga Raju Achary

и другие.

Bioprinting, Год журнала: 2024, Номер 43, С. e00357 - e00357

Опубликована: Сен. 13, 2024

Язык: Английский

Процитировано

1

Biogenic gold nanoparticles stabilized with silk sericin and their impacts on osteogenic induction and surface modification of polymethyl methacrylate DOI

Nathakrit Kasemsuk,

Pimduen Rungsiyakull,

Dakrong Pissuwan

и другие.

Applied Surface Science, Год журнала: 2024, Номер unknown, С. 161591 - 161591

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

1

Silk Sericin/Chitosan Supramolecular Multilayered Thin Films as Sustainable Cytocompatible Nanobiomaterials DOI

Miguel Padilla Rosas,

Cristiana F. V. Sousa,

Ana Pereira

и другие.

Biomacromolecules, Год журнала: 2024, Номер 26(1), С. 296 - 310

Опубликована: Дек. 16, 2024

Silk sericin (SS) has been widely discarded as a waste by the silk textile industry during degumming process to obtain fibroin. However, in past decade, an in-depth understanding of its properties and functions turned it into high added-value biomaterial for biomedical applications. Herein, we report molecular design development sustainable supramolecular multilayered nanobiomaterials encompassing SS oppositely charged chitosan (CHT) through combination self-assembly electrostatically driven layer-by-layer (LbL) assembly technology. The successful buildup SS/CHT was demonstrated quartz crystal microbalance with dissipation monitoring attenuated total reflectance-Fourier transform infrared spectroscopy, nanofilms' wettable nanofibrillar-like topography were shown water contact angle, atomic force microscopy, scanning electron microscopy. In vitro assays cytocompatibility LbL nanofilms toward human primary dermal fibroblasts, holding great promise biofunctional nanocoatings drug/therapeutics/cell delivery, tissue engineering, regenerative medicine.

Язык: Английский

Процитировано

1