Development of a simplified and scalable hydrogel-based method for 3D cell culture DOI Creative Commons

Bin Zhao,

Zexu Dang,

Lingling Li

и другие.

Science Progress, Год журнала: 2025, Номер 108(2)

Опубликована: Апрель 1, 2025

Objective To develop a cost-effective and mechanically robust 3D collagen hydrogel system suitable for pressure-based culture, enabling physiologically relevant in vitro modeling of mechanical stress responses cells. Methods A rat tail type I collagen-based was formulated through optimized component ratios cast into standard 24-well plates to form uniform gel columns. Endothelial cells embedded subjected 30 mmHg pressure culture up 48 h. Gel morphology fiber architecture were assessed via scanning electron microscopy. Cell viability, proliferation (Ki67 immunostaining), tube formation ability evaluated. custom compression setup used apply monitor sustained pressure. Results The exhibited stable gelation, porosity, resistance deformation under loading. SEM confirmed consistent nanofiber network, with diameter unaffected by After 24-h the retained its height structure. remained viable but showed reduced impaired pressure, as indicated Ki67 staining angiogenesis assays. Conclusions This provides simple, cost-effective, scalable alternative complex bioprinting methods, supporting broader application cell biomedical research.

Язык: Английский

Collagen-Based Wound Dressings: Innovations, Mechanisms, and Clinical Applications DOI Creative Commons

Adina Alberts,

Ana‐Maria Bratu, Adelina-Gabriela Niculescu

и другие.

Gels, Год журнала: 2025, Номер 11(4), С. 271 - 271

Опубликована: Апрель 5, 2025

Collagen-based wound dressings have developed as an essential component of contemporary care, utilizing collagen’s inherent properties to promote healing. This review thoroughly analyzes collagen dressing advances, examining different formulations such hydrogels, films, and foams that enhance care. The important processes by which promotes healing (e.g., promoting angiogenesis, encouraging cell proliferation, offering structural support) are discussed clarify its function in tissue regeneration. effectiveness adaptability demonstrated via clinical applications investigated acute chronic wounds. Additionally, commercially accessible collagen-based skin treatments discussed, demonstrating their practical use healthcare settings. Despite the progress, study discusses obstacles restrictions encountered producing adopting dressings, difficulties manufacturing financial concerns. Finally, current landscape’s insights indicate future research possibilities for optimization, bioactive agent integration, overcoming existing constraints. analysis highlights potential innovations improve treatment methods patient

Язык: Английский

Процитировано

2
3D bioprinted alginate/gelatin hydrogel: concentration modulated properties toward scar-minimized wound healing DOI
Tian Jiao, Chaofan Sun, Zhuo Wang

и другие.

Journal of Biomaterials Science Polymer Edition, Год журнала: 2025, Номер unknown, С. 1 - 22

Опубликована: Апрель 16, 2025

The critical shortage of transplantable skin remains a leading cause mortality in patients with severe injuries, driving the demand for advanced 3D-bioprinted constructs. While hydrogel-based bioinks are pivotal tissue engineering, existing systems often fail to simultaneously address biomechanical compatibility, scar suppression, and cell viability. Here, we propose rationally designed sodium alginate/gelatin (SA/Gel) hydrogel platform through composition-property-performance correlation analysis. Systematic characterization revealed that increasing gelatin content (8-12 wt%) enhanced viscosity (by 2.5-fold), compressive modulus (25.6 ± 2.7 kPa 37.9 3.5 kPa), tensile fracture elongation (57.9 4.2% 92.1 1.3%), print fidelity, while reducing degradation ratio (62.8 2.9% 26.4 2.4% at day 14) pore size (128.5 16.6 μm 79.4 19.7 μm). optimized A4G10 formulation exhibited synergistic advantages: (1) dynamic swelling (36.3 0.8%) balanced nutrient permeation structural stability; (2) tunable (47.2% matched neo-tissue formation; (3) anisotropic mechanical properties (compressive 32.2 4.1 kPa, 31.7 3.9 kPa) mimicked native mechanics; (4) sub-100 porous architecture (102.9 12.4 μm) effectively suppressed fibroblast over--proliferation. Remarkably, SA/Gel scaffolds maintained 98% viability (Live/Dead assay) vitro, suppressing fibrotic formation facilitating angiogenesis vivo. This multi-functional system demonstrates unprecedented potential as scar--inhibiting bioink clinical-grade regeneration.

Язык: Английский

Процитировано

0
Tendon regeneration deserves better: focused review on In vivo models, artificial intelligence and 3D bioprinting approaches DOI Creative Commons
Damla Aykora, Burak Taşçı,

Muhammed Zahid Şahin

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Апрель 25, 2025

Tendon regeneration has been one of the most challenging issues in orthopedics. Despite various surgical techniques and rehabilitation methods, tendon tears or ruptures cannot wholly regenerate gain load-bearing capacity tissue had before injury. The enhancement mostly requires grafting an artificial tendon-like to replace damaged tendon. engineering offers promising regenerative effects with numerous additive manufacturing context. 3D bioprinting is a widely used method produce tissues based on biocompatible substitutes. There are multiple bio-inks for fabricating innovative scaffolds applications. Nevertheless, there still many drawbacks overcome successful injured tissue. important target catch highest similarity requirements such as anisotropy, porosity, viscoelasticity, mechanical strength, cell-compatible constructs. To achieve best-designed structure, novel AI-based systems field may unveil excellent final products re-establish integrity functionality. AI-driven optimization can enhance bio-ink selection, scaffold architecture, printing parameters, ensuring better alignment biomechanical properties native tendons. Furthermore, AI algorithms facilitate real-time process monitoring adaptive adjustments, improving reproducibility precision fabrication. Thus, vitro biocompatibility vivo application-based experimental processes will make it possible accelerate healing reach required strength. Integrating predictive modeling further refine these evaluate performance, cell viability, durability, ultimately translation into clinical Here this review, approaches technology incorporation were given addition models.

Язык: Английский

Процитировано

0
Development of a simplified and scalable hydrogel-based method for 3D cell culture DOI Creative Commons

Bin Zhao,

Zexu Dang,

Lingling Li

и другие.

Science Progress, Год журнала: 2025, Номер 108(2)

Опубликована: Апрель 1, 2025

Objective To develop a cost-effective and mechanically robust 3D collagen hydrogel system suitable for pressure-based culture, enabling physiologically relevant in vitro modeling of mechanical stress responses cells. Methods A rat tail type I collagen-based was formulated through optimized component ratios cast into standard 24-well plates to form uniform gel columns. Endothelial cells embedded subjected 30 mmHg pressure culture up 48 h. Gel morphology fiber architecture were assessed via scanning electron microscopy. Cell viability, proliferation (Ki67 immunostaining), tube formation ability evaluated. custom compression setup used apply monitor sustained pressure. Results The exhibited stable gelation, porosity, resistance deformation under loading. SEM confirmed consistent nanofiber network, with diameter unaffected by After 24-h the retained its height structure. remained viable but showed reduced impaired pressure, as indicated Ki67 staining angiogenesis assays. Conclusions This provides simple, cost-effective, scalable alternative complex bioprinting methods, supporting broader application cell biomedical research.

Язык: Английский

Процитировано

0