Journal of Neuroscience,
Год журнала:
2015,
Номер
35(41), С. 13836 - 13842
Опубликована: Окт. 14, 2015
The
mammalian
target
of
rapamycin
(mTOR)
is
a
central
regulator
diverse
array
cellular
processes,
including
cell
growth,
proliferation,
autophagy,
translation,
and
actin
polymerization.
Components
the
mTOR
cascade
are
present
at
synapses
influence
synaptic
plasticity
spine
morphogenesis.
A
prevailing
view
that
study
its
role
in
autism
spectrum
disorders
(ASDs)
will
elucidate
molecular
mechanisms
by
which
regulates
neuronal
function
under
physiological
pathological
conditions.
Although
many
ASDs
arise
as
result
mutations
genes
with
multiple
functions,
they
appear
to
converge
on
common
biological
pathways
give
rise
autism-relevant
behaviors.
Dysregulation
signaling
has
been
identified
phenotypic
feature
fragile
X
syndrome,
tuberous
sclerosis
complex
1
2,
neurofibromatosis
1,
phosphatase
tensin
homolog,
potentially
Rett
syndrome.
Below
summary
topics
covered
symposium
presents
dysregulation
unifying
theme
subset
ASDs.
Nature Communications,
Год журнала:
2015,
Номер
6(1)
Опубликована: Сен. 24, 2015
Abstract
Single-cell
analysis
reveals
aspects
of
cellular
physiology
not
evident
from
population-based
studies,
particularly
in
the
case
highly
multiplexed
methods
such
as
mass
cytometry
(CyTOF)
able
to
correlate
levels
multiple
signalling,
differentiation
and
cell
fate
markers.
Immunofluorescence
(IF)
microscopy
adds
information
on
morphology
microenvironment
that
are
obtained
using
flow-based
techniques,
but
multiplicity
conventional
IF
is
limited.
This
has
motivated
development
imaging
require
specialized
instrumentation,
exotic
reagents
or
proprietary
protocols
difficult
reproduce
most
laboratories.
Here
we
report
a
public-domain
method
for
achieving
high
single-cell
cyclic
immunofluorescence
(CycIF),
simple
versatile
procedure
which
four-colour
staining
alternates
with
chemical
inactivation
fluorophores
progressively
build
multichannel
image.
Because
CycIF
uses
standard
instrumentation
no
more
expensive
than
IF,
it
suitable
high-throughput
assays
screening
applications.
Cell Research,
Год журнала:
2018,
Номер
28(3), С. 281 - 295
Опубликована: Фев. 9, 2018
The
mitochondrial
network
is
not
only
required
for
the
production
of
energy,
essential
cofactors
and
amino
acids,
but
also
serves
as
a
signaling
hub
innate
immune
apoptotic
pathways.
Multiple
mechanisms
have
evolved
to
identify
combat
dysfunction
maintain
health
organism.
One
such
pathway
unfolded
protein
response
(UPR
Rab7
is
a
small
GTPase
that
belongs
to
the
Rab
family
and
controls
transport
late
endocytic
compartments
such
as
endosomes
lysosomes.
The
mechanism
of
action
in
pathway
has
been
extensively
studied.
fundamental
for
lysosomal
biogenesis,
positioning
functions,
trafficking
degradation
several
signaling
receptors,
thus
also
having
implications
on
signal
transduction.
Several
interacting
proteins
have
being
identified
leading
discovery
number
different
important
beside
its
established
role
endocytosis.
Furthermore,
specific
functions
neurons.
This
review
highlights
discusses
importance
cellular
pathways
processes.
