Tongbian formula alleviates slow transit constipation by increasing intestinal butyric acid to activate the 5-HT signaling

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 2, 2024

Slow transit constipation (STC) is a long-lasting and prevalent intestinal condition, marked by hard, dry feces. The primary cause of STC may be attributed to an imbalance in the gut's microbial community alterations its metabolic byproducts. Tongbian formula (TB), traditional Chinese medicinal formula, has been used treat shows great effect on relieving constipation. role TB regulating microbiota not fully elucidated. Herein, we investigated potential gut further explored mechanism behind effects. Our study demonstrated that significantly increased fecal water content ink propulsion rate loperamide (Lope)-induced rats. 5-HT signaling was suppressed colon tissue, abundance butyric acid (BA) colonic contents down-regulated after Lope treatment. Notably, administration led restoration dysbiosis up-regulation BA content, subsequently activating pathways. When combined with tryptophan hydroxylase-1 (TPH1) inhibitor, which crucial for synthesis, therapeutic efficacy treating compromised. alleviates reversing through increasing levels. These findings suggest ideal candidate

Язык: Английский

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Wuzhuyu Decoction relieves hyperalgesia by regulating central and peripheral 5-HT in chronic migraine model rats

Phytomedicine, Год журнала: 2021, Номер 96, С. 153905 - 153905

Опубликована: Дек. 25, 2021

Chronic migraine (CM) is a highly disabling and burdensome disease. Wuzhuyu decoction (WZYD), clinical used formula to treat prevent episodic CM, has been reported relieve the hyperalgesia of CM increase brainstem blood serotonin (5-hydroxytryptamine, 5-HT) in model rats previous studies; yet mechanism unclear.This study aimed observe relief effect WZYD investigate mechanistic association with regulation on central peripheral 5-HT.WZYD different doses (3.372, 1.686 0.843 g/kg∙d) positive drug - sumatriptan (5.83 mg/kg∙3 d) were intragastrically administered inflammatory soup (IS)-induced rats, respectively. Hyperalgesia was assessed by facial mechanical withdrawal threshold tail-flick latency. 5-HT determined ELISA. Western blot analysis, immunohistochemistry immunofluorescence determination, 16S rRNA gene sequencing performed.WZYD significantly relieved elevating In groups, increased decreased calcitonin gene-related peptide both plasma, downregulated TNF-α, IL-1β, c-fos expression observed dose-dependent manner. Interestingly, colon tissues also observed, which associated upregulating tryptophan hydroxylase, transporter Piezo1 increasing chromogranin A enterochromaffin cells. Disorder microbiota, function metabolism correlated synthesis. could regulate abundance Anaerostipes Acidifaciens.WZYD pharmacological relieving possibly affecting 5-HT.

Язык: Английский

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RET Signaling Persists in the Adult Intestine and Stimulates Motility by Limiting PYY Release From Enteroendocrine Cells

Gastroenterology, Год журнала: 2023, Номер 166(3), С. 437 - 449

Опубликована: Ноя. 21, 2023

Язык: Английский

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Modulating cecal microbiome and in silico amino acid metabolism of sanguinarine-based isoquinoline alkaloids supplements in natural heat stress broiler

Livestock Science, Год журнала: 2024, Номер 283, С. 105441 - 105441

Опубликована: Март 11, 2024

Broilers reared under tropical conditions are exposed to Heat Stress (HS) yielding adverseness in production. The study aimed evaluate the effect of plant-derived sanguinarine-based Isoquinoline alkaloids (IQ) on integrating performance growth with cecal microbiome and functional metabolism profiles amino acid for either precision nutrition or productivity promotion using strategies intestinal microecological balance broilers 14-and 35-day age HS conditions. IQ significantly improved performance, body weight, average daily weight gain, feed intake, mortality. modulated cecum (14- 35-dayo age) by enriching diversity increase Bacteriodetes Cyanobacteria, but decrease Firmicutes Proteobacteria. It was found 14-day that abundances Turicibactor sanguinis, Lactobacillus salivarious, sacidophilus, Akkermansia municiphila boiler fed IQ60 were higher than control group. While Enteroccucus cecorum, Enteroccuus villorum, Escherichia fergusonii, Helicobacter pullorum lower broiler IQs demonstrating potential suppress Antimicrobial-Resistant (AMR) reservoirs cecum. increased namely, L-arginine degradation VII L-tryptophan I, VI day 14, L-alanine VII, biosynthesis, L-methionine biosynthesis salvage cycle, L-threonine II 35 (Kruskal-Wallis p-value < 0.05) which positively correlated BW FI negative correlation FCR (Pearson correlation, P<0.05). is first finding potentially crosstalk between host microbiota via microbiome-gut-brain axis gut homeostasis improving Turicibacter sanguini, promoted serotonin from colonic Enterochromaffin Cells (EC) modulating Gastro-Intestinal tract (GI) motility. can be concluded exhibited modulate improve pathways supporting stress broilers. phenotypic activity related genotype prediction silico model systems multi-OMICs methods relating community worthwhile further investigated leading discovering phytogenic compounds additives

Язык: Английский

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Common Pathophysiological Mechanisms and Treatment of Diabetic Gastroparesis

Journal of Neurogastroenterology and Motility, Год журнала: 2024, Номер 30(2), С. 143 - 155

Опубликована: Апрель 5, 2024

Diabetic gastroparesis (DGP) is a common complication of diabetes mellitus, marked by gastrointestinal motility disorder, delayed gastric emptying present in the absence mechanical obstruction.Clinical manifestations include postprandial fullness and epigastric discomfort, bloating, nausea, vomiting.DGP may significantly affect quality life productivity patients.Research on relationship between dynamics DGP has received much attention because increasing prevalence DGP.Gastrointestinal disorders are closely related to variety factors including destruction interstitial cells Cajal, abnormalities neuro-endocrine system hormone levels.Therefore, this study will review recent literature mechanisms as well development prokinetic treatment order give future research directions identify strategies for DGP. (

Язык: Английский

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