Exploring the effects of gut microbiota on cholangiocarcinoma progression by patient-derived organoids

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 9, 2025

Язык: Английский

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Organoid: Bridging the gap between basic research and clinical practice

Cancer Letters, Год журнала: 2023, Номер 572, С. 216353 - 216353

Опубликована: Авг. 18, 2023

Язык: Английский

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Recent Advancement in Diagnosis of Biliary Tract Cancer through Pathological and Molecular Classifications

Cancers, Год журнала: 2024, Номер 16(9), С. 1761 - 1761

Опубликована: Май 1, 2024

Biliary tract cancers (BTCs), including intrahepatic, perihilar, and distal cholangiocarcinomas, as well gallbladder cancer, are a diverse group of that exhibit unique molecular characteristics in each their anatomic pathological subtypes. The classification BTCs compromises distinct growth patterns, mass forming, periductal infiltrating, intraductal growing types, which can be identified through gross examination. small-duct large-duct types intrahepatic cholangiocarcinoma have been recently introduced into the WHO classification. presentation typical clinical symptoms, extensive utilization radiological, endoscopic, diagnostic methods, is thoroughly detailed description. To overcome limitations traditional tissue acquisition new modalities being explored. treatment landscape also rapidly evolving owing to emergence subgroups with alterations corresponding targeted therapies. Furthermore, we emphasize crucial aspects diagnosing BTC practical settings.

Язык: Английский

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Advancing Cholangiocarcinoma Care: Insights and Innovations in T Cell Therapy

Cancers, Год журнала: 2024, Номер 16(18), С. 3232 - 3232

Опубликована: Сен. 23, 2024

Cholangiocarcinoma (CCA) is a rare and aggressive malignancy originating from the bile ducts, with poor prognosis limited treatment options. Traditional therapies, such as surgery, chemotherapy, radiation, have shown efficacy, especially in advanced cases. Recent advancements immunotherapy, particularly T cell-based therapies like chimeric antigen receptor (CAR T) cells, tumor-infiltrating lymphocytes (TILs), cell (TCR)-based opened new avenues for improving outcomes CCA. This review provides comprehensive overview of current state CCA, focusing on CAR therapy. It highlights key challenges, including complex tumor microenvironment immune evasion mechanisms, progress made preclinical clinical trials. The also discusses ongoing trials targeting specific CCA antigens, MUC1, EGFR, CD133, evolving role precision immunotherapy enhancing outcomes. Despite significant progress, further research needed to optimize these solid tumors By summarizing most recent results future directions, this underscores promising potential revolutionizing treatment.

Язык: Английский

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Tumor Immune Microenvironment in Intrahepatic Cholangiocarcinoma: Regulatory Mechanisms, Functions, and Therapeutic Implications

Cancers, Год журнала: 2024, Номер 16(20), С. 3542 - 3542

Опубликована: Окт. 20, 2024

Treatment options for intrahepatic cholangiocarcinoma (iCCA), a highly malignant tumor with poor prognosis, are limited. Recent developments in immunotherapy and immune checkpoint inhibitors (ICIs) have offered new hope treating iCCA. However, several issues remain, including the identification of reliable biomarkers response to ICIs immune-based combinations. Tumor microenvironment (TIME) these hepatobiliary tumors has been evaluated is under assessment this setting order boost efficacy convert immunologically "cold" "hot" tumors. Herein, review TIME ICCA its critical function immunotherapy. Moreover, paper also discusses potential avenues future research, novel targets emerging treatment plans aimed increase effectiveness survival rates iCCA patients.

Язык: Английский

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Infiltrating T lymphocytes and tumor microenvironment within cholangiocarcinoma: immune heterogeneity, intercellular communication, immune checkpoints

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 8, 2025

Cholangiocarcinoma is the second most common primary liver cancer, and its global incidence has increased in recent years. Radical surgical resection systemic chemotherapy have traditionally been standard treatment options. However, complexity of cholangiocarcinoma subtypes often presents a challenge for early diagnosis. Additionally, high recurrence rates following radical resistance to late-stage limit benefits patients. Immunotherapy emerged as an effective strategy treating various types shown efficacy when combined with cholangiocarcinoma. Current immunotherapies targeting predominantly focused on T lymphocytes within tumor microenvironment, new yielded unsatisfactory results clinical trials. Therefore, it essential achieve comprehensive understanding unique microenvironment pivotal role it. In this review, we describe heterogeneous immune landscape intercellular communication summarize specific distribution lymphocytes. Finally, review potential checkpoints

Язык: Английский

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AUTO-SP: automated sample preparation for analyzing proteins and protein modifications

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

ABSTRACT Liquid chromatography (LC) tandem mass spectrometry (MS/MS) is one of the widely used proteomic techniques to study alterations occurred at protein expression level as well post-translation modifications (PTMs) proteins that are relevant different physiological or pathological statuses. The spectrometric analysis peptides fragmented from (bottom-up proteomics) has emerged major approaches for proteomics. In this approach, first cleaved into and with PTMs further enriched followed by LC-MS/MS analysis. To achieve a reproducible quantitative characterization, well-established protease digestion PTM peptide enrichment protocol critical. study, we developed an automated sample preparation (AUTO-SP) analyzing utilizing Patient-Derived Xenograft (PDX) breast cancer tumors (basal-like luminal subtypes). amount was quantified were digested using AUTO-SP each PDX sample. Based on data-independent acquisition (DIA)-MS data, observed samples same subtypes highly correlated (>0.98). Additionally, >14,000 ubiquitinated identified in when ubiquitin enrichment, while unique pathways basal-like subtypes. demonstrated its efficacy provide reliable procedure MS-based analyses.

Язык: Английский

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IL-8–NF-κB–ALDH1A1 loop promotes the progression of intrahepatic cholangiocarcinoma

Hepatology Communications, Год журнала: 2025, Номер 9(3)

Опубликована: Фев. 26, 2025

Background: Intrahepatic cholangiocarcinoma (ICC) is a poor prognosis of malignant cancer with high lymph node metastasis and resistance to systemic therapies. Recent studies suggested that the involvement IL-8 could promote ICC through epithelial–mesenchymal transition while ICC-ALDH1A1 subtype clarified by multi-omics study. The correlation between ALDH1A1 in remains elusive. This study aims further explore roles regulatory mechanisms ICC. Methods: We analyzed expression patients cells. CXCR2 inhibitor (SB225002) was applied inhibit function IL-8, JSH-23 NF-κB signaling pathway. examined effects inhibition on NF-κB, expression, cell growth, migration, invasion, stemness. Moreover, we tumor growth were validated using subcutaneous tumors nude mice. Results: IL-8-derived cells progression. serum associated metastasis. upregulate activating pathway, promoting Upregulation activate secretion, forming positive feedback loop invasiveness stemness Conclusions:

Язык: Английский

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Factors Contributing to the High Malignancy Level of Cholangiocarcinoma and Its Epidemiology: Literature Review and Data

Biology, Год журнала: 2025, Номер 14(4), С. 351 - 351

Опубликована: Март 28, 2025

CCA is a highly desmoplastic malignant cancer and the second most common primary liver malignancy after hepatocellular carcinoma (HCC), accounting for approximately 15% of all tumors. has poor prognosis, with an average five-year survival rate 9%, which lower than that pancreatic cancer. Although considerable efforts have been invested into genomics, epigenetics, risk factors, very little known about what might key causes high level CCA. In this review, we analyze incidence mortality in different regions based on data from 1994 to 2022 obtained International Agency Research Cancer (IARC), discuss current status treatment disease, focus be main factors contributing CCA: alkalosis caused by Fenton reaction, hypoxia, TIME. The review includes studies published 1979 2024, aiming provide updated synthesis basic early classical theoretical knowledge By revealing epidemiological characteristics CCA, potential mechanisms malignancy, challenges treatment, aims new directions future research, promote development personalized strategies, facilitate deeper understanding more effective management worldwide.

Язык: Английский

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Metabolic Reprogramming in Cholangiocarcinoma Cancer Stem Cells: Emerging Therapeutic Paradigms

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217714 - 217714

Опубликована: Апрель 1, 2025

Язык: Английский

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