Cancers,
Год журнала:
2024,
Номер
17(1), С. 27 - 27
Опубликована: Дек. 25, 2024
Background/Objectives:
Despite
the
introduction
of
innovative
therapeutics,
lung
cancer
is
still
leading
cause
cancer-related
death.
For
this
reason,
requires
deep
characterization
to
identify
cellular
and
molecular
targets
that
can
be
used
develop
novel
therapeutic
strategies.
Three-dimensional
models,
including
patient-derived
organoids
(PDOs),
represent
useful
tools
study
biology
may
employed
in
future
as
predictive
decisions.
However,
successful
establishment
cultures
faithfully
respective
patient
tissues
challenging
due
low
success
rate
and/or
overgrowth
normal
airway
epithelial
cells.
Methods:
We
set
up
a
two-step
protocol
allows
for
establishing
both
short-term
long-term
3D
cultures,
with
different
characteristics
rates.
Results:
Cancer
tissue-originated
spheroids
(CTOSs)
show
100%
allow
concomitant
isolation
autologous
tumor
infiltrating
leukocytes
(TILs).
On
contrary,
PDOs
expanded
medium-long
term
bio-banked
but
retain
lower
possibility
contamination
To
overcome
these
problems,
we
an
optimal
medium
formulation
implemented
rigorous
quality
controls,
substantial
improvement
tumoral
PDO
establishment.
Conclusions:
Overall,
guarantees
flexibility
reliability,
also
providing
guidelines
control
checks
support
experimental
settings.
The
setting
robust
culture
expansion
key
requirement
their
employment
research
clinical
practice.
Cancer Letters,
Год журнала:
2024,
Номер
598, С. 217106 - 217106
Опубликована: Июль 9, 2024
Diffuse-type
gastric
cancer
(DGC)
is
a
subtype
of
with
aggressiveness
and
poor
prognosis.
It
great
significance
to
find
sensitive
drugs
for
DGC.
In
the
current
study,
total
20
patient-derived
organoids
(PDOs)
were
analyzed
screening
therapeutic
efficacy
small
molecule
kinases
inhibitors
on
cancers,
especially
difference
between
intestinal-type
(IGCs)
DGCs.
The
IGCs
are
multiple
inhibitors,
while
DGCs
resistant
most
these
inhibitors.
was
found
that
showed
drug-induced
senescent
phenotype
after
treatment
by
aurora
(AURKi)
Barasertib-HQPA
Danusertib.
cell
diameter
cells
increased
stronger
staining
senescence-associated
β-galactosidase
(SA-β-GAL),
characteristic
appearance
multinucleated
giant
cells.
secrete
large
amounts
chemokine
MCP-1/CCL2,
which
recruit
induce
macrophage
M2-type
polarization
in
PDOs
DGC
(DPDOs)-macrophage
co-culture
system.
up-regulation
local
MCP-1/CCL2
can
interact
receptor
(CCR2)
expressed
macrophages
suppress
their
innate
immunity
Overall,
special
response
AURKi
suggests
clinicians
should
select
sequential
therapy
clearance
Abstract
The
alteration
in
the
neural
circuits
of
both
central
and
peripheral
nervous
systems
is
closely
related
to
onset
neurodegenerative
disorders
(NDDs).
Despite
significant
research
efforts,
knowledge
regarding
NDD
pathological
processes,
development
efficacious
drugs
are
still
limited
due
inability
access
reproduce
components
system
its
intricate
microenvironment.
2D
culture
too
simplistic
accurately
represent
more
complex
dynamic
situation
cells
vivo
have
therefore
been
surpassed
by
3D
systems.
However,
models
suffer
from
various
limitations
that
can
be
overcome
employing
two
innovative
technologies:
organ‐on‐chip
printing.
In
this
review,
an
overview
advantages
shortcomings
microfluidic
platforms
extracellular
matrix‐like
biomaterials
will
given.
Then,
combination
microfluidics
hydrogels
as
a
new
synergistic
approach
study
analyzing
latest
advances
brain‐on‐chip
for
explored.
Organoids,
Год журнала:
2025,
Номер
4(1), С. 2 - 2
Опубликована: Янв. 24, 2025
Peritoneal
carcinomatosis
from
gastrointestinal
tumours
is
considered
a
poor
prognostic
factor,
with
median
overall
survival
of
six
to
nine
months
in
the
absence
intervention.
The
advent
patient-derived
organoid
cultures
(PDOs)
has
provided
breakthrough
personalised
medicine,
allowing
researchers
and
clinicians
model
complexity
heterogeneity
individual
vitro.
PDOs
hold
great
promise
this
field,
as
variations
management
peritoneal
due
differences
method
delivery
chemotherapeutics,
drug
selection,
exposure
duration,
tumour
pathology
make
it
impractical
use
single,
standardised
treatment
regimen.
We
aim
summarise
methodologies
limitations
studies
encapsulating
organoids
derived
metastases
encourage
design
considerations
that
may
improve
future
clinical
relevance,
standardise
protocols,
address
translational
challenges
personalising
strategies.
Cellular and Molecular Gastroenterology and Hepatology,
Год журнала:
2024,
Номер
18(3), С. 101366 - 101366
Опубликована: Янв. 1, 2024
Type
2
innate
lymphoid
cells
(ILC2s)
and
interleukin-13
(IL-13)
promote
the
onset
of
spasmolytic
polypeptide-expressing
metaplasia
(SPEM)
cells.
However,
little
is
known
about
molecular
effects
IL-13
in
SPEM
We
now
sought
to
establish
a
reliable
organoid
model,
Meta1
gastroids,
model
vitro.
evaluated
cellular
ILC2s
on
maturation
proliferation
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Окт. 25, 2024
The
human
endometrium
is
a
highly
regenerative
tissue
capable
of
undergoing
scarless
repair
during
the
menstruation
and
postpartum
phases.
This
process
mediated
by
endometrial
adult
stem/progenitor
cells.
During
healing
injuries,
swift
reepithelization
results
in
rapid
covering
wound
surface
facilitates
subsequent
restoration.
involvement
endogenous
epithelial
stem
cells,
stromal
bone
marrow-derived
cells
regeneration
epithelium
has
been
subject
prolonged
debate.
Increasing
evidence
suggests
that
mainly
relies
on
rather
than
Currently,
no
consensus
established
identity
compartment.
Several
markers,
including
stage-specific
embryonic
antigen-1
(SSEA-1),
sex-determining
region
Y-box
9
(SOX9),
neural-cadherin
(N-cadherin),
leucine-rich-repeat-containing
G-protein-coupled
receptor
5
(LGR5),
CD44,
axis
inhibition
protein
2
(Axin2),
aldehyde
dehydrogenase
1A1
(ALDH1A1),
have
suggested
as
potential
candidate
markers
for
identification
contributes
to
our
understanding
offers
new
therapeutic
insights
into
diseases
characterized
defects
endometrium,
such
intrauterine
adhesion.
review
explores
different
perspectives
origins
mouse
It
summarizes
locations,
hierarchies
both
endometrium.
also
discusses
cell-based
treatments
adhesion,
hoping
inspire
further
research
clinical
application
Biomedicines,
Год журнала:
2023,
Номер
11(10), С. 2743 - 2743
Опубликована: Окт. 10, 2023
One
of
the
major
goals
in
advancement
basic
cancer
research
focuses
on
development
new
anticancer
therapies.
To
understand
molecular
mechanisms
progression,
acquired
drug
resistance,
and
metastatic
process,
use
preclinical
vitro
models
that
faithfully
summarize
properties
tumor
patients
is
still
a
necessity.
The
represented
by
diverse
group
cell
clones,
recent
years,
to
reproduce
models,
monolayer
cultures
have
been
supplanted
patient-derived
xenograft
(PDX)
cultured
organoids
derived
from
patient
(PDO).
These
proved
indispensable
for
study
microenvironment
(TME)
its
interaction
with
cells.
Prostate
(PCa)
most
common
neoplasia
men
world.
It
characterized
genomic
instability
resistance
conventional
Despite
advances
diagnosis
treatment,
PCa
remains
leading
cause
death.
Here,
we
review
studies
last
10
years
as
number
papers
growing
very
fast
field.
We
also
discuss
discovered
limitations
challenges
using
organoid
culture
system
PDXs
studying
prostate
phenotype,
performing
testing,
developing
