Development of a Recombinant Omicron BA.1 Subunit Vaccine Candidate in Pichia pastoris
Microbial Biotechnology,
Год журнала:
2025,
Номер
18(1)
Опубликована: Янв. 1, 2025
ABSTRACT
Low‐cost
and
safe
vaccines
are
needed
to
fill
the
vaccine
inequity
gap
for
future
pandemics.
Pichia
pastoris
is
an
ideal
expression
system
recombinant
protein
production
due
its
cost‐effective
easy‐to‐scale‐up
process.
Here,
we
developed
a
next‐generation
SARS‐CoV2
Omicron
BA.1‐based
candidate
expressed
in
P.
.
The
receptor
binding
domain
of
BA.1
spike
(RBD‐Omicron)
was
produced
at
0.35
g/L
supernatant.
With
60%
recovery
after
two‐step
purification,
RBD‐Omicron
showed
99%
purity.
After
vitro
characterisation
purified
via
chromatography,
mass
spectrometry,
calorimetry
surface
plasmon
resonance‐based
methods,
it
injected
into
mice
immunization
studies.
Three
different
doses
Alum
CpG
adjuvanted
were
investigated
10
μg
gave
highest
antigenicity.
two
vaccination,
IgG
titers
serum
reached
more
than
6
These
antibodies
also
recognized
earlier
(Delta
Plus:
B.1.617.2)
later
(Eris:
EG.5,
Pirola:
BA.2.86)
variants.
long‐term
immunological
response
measured
by
analyzing
antibody
T‐cell
splenocytes
60
weeks.
Interestingly,
Th1
significantly
high
even
year.
subvariants
dominantly
circulating
world,
so
sub‐lineage‐based
can
be
used
RBD‐Omicron‐based
this
study
suitable
technology
transfer
transition
clinic.
Язык: Английский
Presence of EGF ligand restricts the binding ability of EgB4 nanobody to EGFR extracellular domain
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 18, 2025
Язык: Английский
Viral entry mechanisms: the role of molecular simulation in unlocking a key step in viral infections
FEBS Open Bio,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 14, 2024
Viral
infections
are
a
major
global
health
concern,
affecting
millions
of
people
each
year.
entry
is
one
the
crucial
stages
in
infection
process,
but
its
details
remain
elusive.
Enveloped
viruses
enclosed
by
lipid
membrane
that
protects
their
genetic
material
and
these
linked
to
various
human
illnesses,
including
influenza,
COVID‐19.
Due
advancements
made
field
molecular
simulation,
significant
progress
has
been
unraveling
dynamic
processes
involved
viral
enveloped
viruses.
Simulation
studies
have
provided
deep
insight
into
function
proteins
responsible
for
attaching
host
receptors
promoting
fusion
(fusion
proteins),
deciphering
interactions
between
receptors,
shedding
light
on
functional
significance
key
regions,
such
as
peptide.
These
already
significantly
contributed
our
understanding
this
critical
aspect
assisted
development
effective
strategies
combat
diseases
improve
health.
This
review
focuses
vital
role
facilitating
process
highlights
contributions
simulation
uncover
underlying
mechanisms
action.
Язык: Английский