Viral entry mechanisms: the role of molecular simulation in unlocking a key step in viral infections DOI Creative Commons
Mariana Valério, Carolina C. Buga, Manuel N. Melo

et al.

FEBS Open Bio, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Viral infections are a major global health concern, affecting millions of people each year. entry is one the crucial stages in infection process, but its details remain elusive. Enveloped viruses enclosed by lipid membrane that protects their genetic material and these linked to various human illnesses, including influenza, COVID‐19. Due advancements made field molecular simulation, significant progress has been unraveling dynamic processes involved viral enveloped viruses. Simulation studies have provided deep insight into function proteins responsible for attaching host receptors promoting fusion (fusion proteins), deciphering interactions between receptors, shedding light on functional significance key regions, such as peptide. These already significantly contributed our understanding this critical aspect assisted development effective strategies combat diseases improve health. This review focuses vital role facilitating process highlights contributions simulation uncover underlying mechanisms action.

Language: Английский

Presence of EGF ligand restricts the binding ability of EgB4 nanobody to EGFR extracellular domain DOI Creative Commons

Duc Toan Truong,

Mateusz Chwastyk,

Thi Viet

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 18, 2025

Language: Английский

Citations

1
Development of a Recombinant Omicron BA.1 Subunit Vaccine Candidate in Pichia pastoris DOI Creative Commons
Sibel Kalyoncu,

Dogu Sayili,

Ayça Zeybek Kuyucu

et al.

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 1, 2025

ABSTRACT Low‐cost and safe vaccines are needed to fill the vaccine inequity gap for future pandemics. Pichia pastoris is an ideal expression system recombinant protein production due its cost‐effective easy‐to‐scale‐up process. Here, we developed a next‐generation SARS‐CoV2 Omicron BA.1‐based candidate expressed in P. . The receptor binding domain of BA.1 spike (RBD‐Omicron) was produced at 0.35 g/L supernatant. With 60% recovery after two‐step purification, RBD‐Omicron showed 99% purity. After vitro characterisation purified via chromatography, mass spectrometry, calorimetry surface plasmon resonance‐based methods, it injected into mice immunization studies. Three different doses Alum CpG adjuvanted were investigated 10 μg gave highest antigenicity. two vaccination, IgG titers serum reached more than 6 These antibodies also recognized earlier (Delta Plus: B.1.617.2) later (Eris: EG.5, Pirola: BA.2.86) variants. long‐term immunological response measured by analyzing antibody T‐cell splenocytes 60 weeks. Interestingly, Th1 significantly high even year. subvariants dominantly circulating world, so sub‐lineage‐based can be used RBD‐Omicron‐based this study suitable technology transfer transition clinic.

Language: Английский

Citations

0
Viral entry mechanisms: the role of molecular simulation in unlocking a key step in viral infections DOI Creative Commons
Mariana Valério, Carolina C. Buga, Manuel N. Melo

et al.

FEBS Open Bio, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Viral infections are a major global health concern, affecting millions of people each year. entry is one the crucial stages in infection process, but its details remain elusive. Enveloped viruses enclosed by lipid membrane that protects their genetic material and these linked to various human illnesses, including influenza, COVID‐19. Due advancements made field molecular simulation, significant progress has been unraveling dynamic processes involved viral enveloped viruses. Simulation studies have provided deep insight into function proteins responsible for attaching host receptors promoting fusion (fusion proteins), deciphering interactions between receptors, shedding light on functional significance key regions, such as peptide. These already significantly contributed our understanding this critical aspect assisted development effective strategies combat diseases improve health. This review focuses vital role facilitating process highlights contributions simulation uncover underlying mechanisms action.

Language: Английский

Citations

2