A pangenome and pantranscriptome of hexaploid oat DOI Creative Commons
Raz Avni, Nadia Kamal, Lidija Bitz

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 27, 2024

Oat grain is a traditional human food rich in dietary fiber that contributes to improved health. Interest the crop has surged recent years owing its use as basis for plant-based milk analogs. an allohexaploid with large, repeat-rich genome was shaped by subgenome exchanges over evolutionary timescales. In contrast many other cereal species, genomic research oat still at early stage, and surveys of structural diversity gene expression variability are scarce. Here, we present annotated chromosome-scale sequence assemblies 33 wild domesticated oats along atlas across six tissues different developmental stages 23 accessions. We describe interplay subgenomes, accessions tissues. Gene loss hexaploid accompanied compensatory up-regulation remaining homeologs, but this process constrained divergence. Chromosomal rearrangements have significantly impacted breeding. A large pericentric inversion associated flowering explains distorted segregation on chromosome 7D homeologous exchange between chromosomes 2A 2C semidwarf mutant risen prominence Australian elite varieties. The pangeome will promote adoption approaches understanding evolution adaptation accelerate their improvement.

Язык: Английский

One hundred years of comparative genetic and physical mapping in cultivated oat (Avena sativa) DOI Creative Commons
Charlene P. Wight, Victoria Blake, Eric N. Jellen

и другие.

Crop and Pasture Science, Год журнала: 2024, Номер 75(2)

Опубликована: Янв. 19, 2024

Context Researchers have been accumulating information concerning the locations of genes and quantitative trait loci (QTLs) in cultivated oat (Avena sativa L.) for more than 100 years. Aims The aim this work was to create an inventory QTLs found hexaploid produce tools make resource useful. Methods By using positions perfectly matched, single nucleotide polymorphism markers, each centimorgan (cM) location along consensus map assigned a on OT3098 v2 physical GrainGenes database website (https://wheat.pw.usda.gov/jb/?data=/ggds/oat-ot3098v2-pepsico). This then used assign inventory, where possible. Key results A table comparing major genetic maps oats other, 2018 map, chromosomes produced. Genome browser tracks aligning regions were added GrainGenes. Conclusions Many identified mapping could be chromosomes. However, many these are quite long, owing presence large areas reduced recombination. Specific examples patterns recombination between validated gene QTL discussed. Implications These resources will assist researchers performing comparative oat.

Язык: Английский

Процитировано

6
The near-complete genome assembly of hexaploid wild oat reveals its genome evolution and divergence with cultivated oats DOI
Qiang He, Wei Li, Yuqing Miao

и другие.

Nature Plants, Год журнала: 2024, Номер unknown

Опубликована: Дек. 3, 2024

Язык: Английский

Процитировано

2
Genetic Mapping of the Powdery Mildew Resistance Gene Pm13 on Oat (Avena sativa) Chromosome 1D DOI Creative Commons
Selma Schurack,

Steffen Beuch,

Sandy Cowan

и другие.

Plant Breeding, Год журнала: 2024, Номер unknown

Опубликована: Дек. 16, 2024

ABSTRACT Powdery mildew, caused by the biotrophic fungus Blumeria graminis DC. f. sp. avenae , is a widespread disease of oats, especially in temperate regions Western and Central Europe, use resistant varieties most sustainable way to ensure stable yields. Therefore, identification robust effective resistance powdery mildew great interest for oat breedinpg. In contrast race‐specific genes, adult plant (APR) generally considered be more durable. The variety ‘Firth’, as well related such ‘Husky’ or ‘Flämingstip’, contains an unknown APR gene, which was previously located on chromosome 1D using DArT markers. aim this study confirm refine chromosomal location tentatively named Pm13 . To end, two independent experiments were carried out different genetic material under natural infection conditions field: genome‐wide association mapping (GWAS) diverse set 250 lines grown 10 environments QTL HuskyxAVE1284 biparental population three environments. Both approaches identified distal end hexaploid Sang genome. locus explained up 15% phenotypic variance GWAS 64% mapping. Comparison field data with results from laboratory leaf segment tests confirmed that does indeed confer APR. sequence information linked markers may allow development molecular useful early selection high levels

Язык: Английский

Процитировано

2
Genetic mapping of the powdery mildew resistance gene Pm13 on oat chromosome 1D DOI Open Access
Selma Schurack,

Steffen Beuch,

Sandy Cowan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 29, 2024

Abstract Powdery mildew, caused by the biotrophic fungus Blumeria graminis DC. f. sp. avenae , is a widespread disease of oats, especially in temperate regions Western and Central Europe, use resistant varieties most sustainable way to ensure stable yields. Therefore, identification robust effective resistance powdery mildew great interest for oat breeding. In contrast race-specific genes, adult plant (APR) generally considered be more durable. The variety Firth, as well related such Husky or Flämingstip, contains an unknown APR gene, which was previously located on chromosome 1D using DArT markers. aim this study confirm refine chromosomal location tentatively named Pm13. To end, two independent experiments were carried out different genetic material under natural infection conditions field: genome-wide association mapping (GWAS) diverse set 250 lines grown ten environments QTL HuskyxAVE1284 bi-parental population three environments. Both approaches identified distal end hexaploid Sang genome. locus explained up 15 % phenotypic variance GWAS 64 mapping. Comparison field data with results from laboratory leaf segment tests confirmed that Pm13 does indeed confer APR. sequence information linked markers may allow development molecular useful early selection high levels

Язык: Английский

Процитировано

1
A pangenome and pantranscriptome of hexaploid oat DOI Creative Commons
Raz Avni, Nadia Kamal, Lidija Bitz

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 27, 2024

Oat grain is a traditional human food rich in dietary fiber that contributes to improved health. Interest the crop has surged recent years owing its use as basis for plant-based milk analogs. an allohexaploid with large, repeat-rich genome was shaped by subgenome exchanges over evolutionary timescales. In contrast many other cereal species, genomic research oat still at early stage, and surveys of structural diversity gene expression variability are scarce. Here, we present annotated chromosome-scale sequence assemblies 33 wild domesticated oats along atlas across six tissues different developmental stages 23 accessions. We describe interplay subgenomes, accessions tissues. Gene loss hexaploid accompanied compensatory up-regulation remaining homeologs, but this process constrained divergence. Chromosomal rearrangements have significantly impacted breeding. A large pericentric inversion associated flowering explains distorted segregation on chromosome 7D homeologous exchange between chromosomes 2A 2C semidwarf mutant risen prominence Australian elite varieties. The pangeome will promote adoption approaches understanding evolution adaptation accelerate their improvement.

Язык: Английский

Процитировано

1