Exploring the subcellular distribution of mercury in green alga Chlamydomonas reinhardtii and diatom Cyclotella meneghiniana: A comparative study

Aquatic Toxicology, Год журнала: 2024, Номер 267, С. 106836 - 106836

Опубликована: Янв. 11, 2024

Mercury (Hg) is a priority pollutant of global concern because its toxicity, ability to bioaccumulate throughout the food web and reach significant concentrations in top predators. Phytoplankton bioconcentrate large amounts Hg play key role entry into aquatic web. However, subcellular distribution freshwater phytoplankton, known affect it toxicity trophic transfer understudied. The present study aimed at investigating accumulation inorganic (iHg) phytoplankton species. To this end green alga Chlamydomonas reinhardtii diatom Cyclotella meneghiniana were exposed 10 100 nM iHg for 2 h. adsorbed, intracellular (granules, debris, organelles, heat-stable peptides (HSP) heat-denaturable proteins (HDP)) fractions determined. results showed that C. accumulated more compared both exposure (10 nM: 4.41 ± 0.74 vs. 1.10 0.25 amol cell

Язык: Английский

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Transformations of aquatic mercury species by the diatom Cyclotella meneghiniana

Environmental Pollution, Год журнала: 2025, Номер 374, С. 126248 - 126248

Опубликована: Апрель 12, 2025

Язык: Английский

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Light-independent phytoplankton degradation and detoxification of methylmercury in water

Nature Water, Год журнала: 2023, Номер 1(8), С. 705 - 715

Опубликована: Авг. 7, 2023

Язык: Английский

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Mercury transformations in algae, plants, and animals: The occurrence, mechanisms, and gaps

The Science of The Total Environment, Год журнала: 2023, Номер 911, С. 168690 - 168690

Опубликована: Ноя. 23, 2023

Язык: Английский

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Interaction of Naturally Occurring Phytoplankton with the Biogeochemical Cycling of Mercury in Aquatic Environments and Its Effects on Global Hg Pollution and Public Health

Microorganisms, Год журнала: 2023, Номер 11(8), С. 2034 - 2034

Опубликована: Авг. 8, 2023

The biogeochemical cycling of mercury in aquatic environments is a complex process driven by various factors, such as ambient temperature, seasonal variations, methylating bacteria activity, dissolved oxygen levels, and Hg interaction with organic matter (DOM). As consequence, part the contamination from anthropogenic activity that was buried sediments reinserted into water columns mainly highly toxic forms (methylmercury, dimethylmercury, etc.). This especially prominent coastal shallow waters industrial regions worldwide. main entrance point these food web naturally occurring phytoplankton. availability, intake, effect on population size, cell toxicity, eventual biotransformation, intracellular stability phytoplankton are greatest importance for human health, having mind incorporated inside cells due to biomagnification effects eventually ends up wildlife, fish, seafood, diet. review summarizes recent findings topic form natural offers new insight possible directions future research prevention scope climate change global pollution increase scenarios.

Язык: Английский

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Unraveling the impact of phytoplankton secretions on the behavior of metal-containing engineered nanoparticles in aquatic environment

Frontiers in Environmental Science, Год журнала: 2024, Номер 11

Опубликована: Янв. 4, 2024

Based on the up-to-date knowledge we critically discuss current understanding of influence compounds secreted by phytoplankton species fate metal-containing engineered nanoparticles (ENPs) in aquatic settings. Different biomolecules, such as extracellular polymeric substances (EPS) and exometabolites play important, yet to elucidate, role dissolution, colloidal stability, transformations biouptake ENPs thus shape their behavior within phycosphere. Phytoplankton secretions can also mediate synthesis from dissolved ions reducing metals capping newly formed ENPs. However, environmental significance this process remains be demonstrated. Exposure triggers changes secretion biomolecules. An improved regulatory mechanism exometabolite due ENP exposure is essential for deciphering ENPs-phytoplankton interactions. Unveiling biomolecules modulating central understudying phytoplankton-ENPs feedbacks, drivers mechanisms environment.

Язык: Английский

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Biotic transformation of methylmercury at the onset of the Arctic spring bloom

Progress In Oceanography, Год журнала: 2024, Номер 222, С. 103224 - 103224

Опубликована: Фев. 18, 2024

Despite the lack of local anthropogenic mercury sources, methylated (MeHg) concentrations in Arctic biota are higher than from lower latitudes. The main entry route occurs during bioconcentration seawater monomethylmercury (MMHg) into phytoplankton. known seasonal changes biological activity region, little is about cycling total (THg) and MeHg Ocean. Here, we report THg sampled northwestern Barents Sea water column late winter spring. In upper 500 m, significantly spring (0.64 ± 0.09 pmol L-1) compared to (0.53 0.07 L-1), driven by inputs surface waters atmospheric deposition dynamics changing sea ice conditions. Contrastingly, were (41 39 fmol (85 42 L-1). We suggest that most biotically demethylated both phytoplankton bacteria, with additional losses photodemethylation evasion. Our observations highlight importance demethylation potential uptake methylmercury coinciding bloom. Lastly, use our new data together previously published region construct a simplified cycle an marginal zone.

Язык: Английский

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Bioconcentration of Inorganic and Methyl Mercury by Algae Revealed Using Dual-Mass Single-Cell ICP-MS with Double Isotope Tracers

Environmental Science & Technology, Год журнала: 2024, Номер 58(18), С. 7860 - 7869

Опубликована: Апрель 22, 2024

Algae are an entry point for mercury (Hg) into the food web. Bioconcentration of Hg by algae is crucial its biogeochemical cycling and environmental risk. Herein, considering cell heterogeneity, we investigated bioconcentration coexisting isotope-labeled inorganic (199IHg) methyl (201MeHg) six typical freshwater marine using dual-mass single-cell inductively coupled plasma mass spectrometry (scICP-MS). First, a universal pretreatment procedure scICP-MS analysis was developed. Using proposed method, intra- interspecies heterogeneities kinetics were revealed at level. The heterogeneity in cellular contents largely related to size. process reached dynamic equilibrium involving influx/adsorption efflux/desorption within hours. Algal density key factor affecting distribution between ambient water. Cellular negatively correlated with algal density, whereas volume concentration factors almost remained constant. Accordingly, developed model based on that well describes density-driven effects algae. From novel perspective, findings improve our understanding governed various biological factors.

Язык: Английский

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Surface displayed MerR increases mercury accumulation by green microalga Chlamydomonas reinhardtii

Environment International, Год журнала: 2024, Номер 189, С. 108813 - 108813

Опубликована: Июнь 12, 2024

Mercury is a highly toxic trace metal that can accumulate in aquatic ecosystems and when resent at high concentrations pose risks to both life humans consuming contaminated fish. This research explores the use of metalloregulatory protein MerR, known for its affinity selectivity toward mercury, novel application. Through cell surface engineering approach, MerR was displayed on cells green alga Chlamydomonas reinhardtii. A hydroxyproline-rich GP1 used as an anchor construct engineered strains GP1-MerR expresses fluorescent mVenus. The strain led up five folds higher Hg

Язык: Английский

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Effect of mercury in the influx and efflux of nutrients in the microalga Desmodesmus armatus

Aquatic Toxicology, Год журнала: 2023, Номер 258, С. 106496 - 106496

Опубликована: Март 19, 2023

Anthropogenic activities such as mining and the metallurgical industry are main sources of mercury contamination. Mercury is one most serious environmental problems in world. This study aimed to investigate, using experimental kinetic data, effect different inorganic (Hg2+) concentrations on response microalga Desmodesmus armatus stress. Cell growth, nutrients uptake ions from extracellular medium, oxygen production were determined. A Compartment Structured Model allowed elucidating phenomena transmembrane transport, including influx efflux nutrients, metal bioadsorption cell wall, which difficult determine experimentally. model was able explain two tolerance mechanisms against mercury, first adsorption Hg2+ions onto wall second ions. The predicted a competition between internalization with maximum tolerable concentration 5.29 mg/L HgCl2. data showed that causes physiological changes cell, allow adapt these new conditions counteract toxic effects. For this reason, D. can be considered Hg-tolerant microalga. capacity associated activation detoxification mechanism facilitates maintenance osmotic balance for all modeled chemical species. Furthermore, accumulation membrane suggests presence thiol groups its internalization, leading conclusion metabolically active dominant over passive ones.

Язык: Английский

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