Animal Reproduction,
Год журнала:
2024,
Номер
21(3)
Опубликована: Янв. 1, 2024
Sperm
quality
is
essential
to
guarantee
the
success
of
assisted
reproduction.
However,
selecting
high-quality
sperm
and
maintaining
it
during
(cryo)preservation
for
high
efficiency
remains
challenging
in
livestock
A
comprehensive
understanding
biology
allows
better
assessment
quality,
which
could
replace
conventional
analyses
used
today
predict
fertility
with
low
accuracy.
Omics
approaches
have
revealed
numerous
biomarkers
associated
various
phenotypic
traits
such
as
survival
storage,
freezability,
fertility.
At
same
time,
nanotechnology
emerging
a
new
biotechnology
potential
use
preparing
intended
improve
reproduction
livestock.
The
unique
physicochemical
properties
nanoparticles
make
them
exciting
tools
targeting
(e.g.,
damage
sexing)
non-targeting
bioapplications.
Recent
advances
led
discovery
biomarkers,
making
possible
target
specific
subpopulations
spermatozoa
within
ejaculate.
In
this
review,
we
explore
phenotypes
highlight
benefits
combining
these
further
preparation
technology.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(5), С. 4656 - 4656
Опубликована: Фев. 28, 2023
Cryopreservation
is
an
expanding
strategy
to
allow
not
only
fertility
preservation
for
individuals
who
need
such
procedures
because
of
gonadotoxic
treatments,
active
duty
in
dangerous
occupations
or
social
reasons
and
gamete
donation
couples
where
conception
denied,
but
also
animal
breeding
endangered
species.
Despite
the
improvement
semen
cryopreservation
techniques
worldwide
expansion
banks,
damage
spermatozoa
consequent
impairment
its
functions
still
remain
unsolved
problems,
conditioning
choice
technique
assisted
reproduction
procedures.
Although
many
studies
have
attempted
find
solutions
limit
sperm
following
identify
possible
markers
susceptibility,
research
this
field
required
order
optimize
process.
Here,
we
review
available
evidence
regarding
structural,
molecular
functional
occurring
cryopreserved
human
strategies
prevent
it
Finally,
results
on
(ARTs)
outcomes
use
spermatozoa.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(20), С. 11124 - 11124
Опубликована: Окт. 16, 2024
Recent
years
have
witnessed
significant
advancements
in
the
cryopreservation
of
various
tissues
and
cells,
yet
several
challenges
persist.
This
review
evaluates
current
state
cryopreservation,
focusing
on
contemporary
methods,
notable
achievements,
ongoing
difficulties.
Techniques
such
as
slow
freezing
vitrification
enabled
successful
preservation
diverse
biological
materials,
including
embryos
ovarian
tissue,
marking
substantial
progress
reproductive
medicine
regenerative
therapies.
These
achievements
highlight
improved
post-thaw
survival
functionality
cryopreserved
samples.
However,
there
are
remaining
ice
crystal
formation,
which
can
lead
to
cell
damage,
larger,
more
complex
organs.
also
explores
role
cryoprotectants
importance
optimizing
both
cooling
warming
rates
enhance
outcomes.
Future
research
priorities
include
developing
new
cryoprotective
agents,
elucidating
mechanisms
cryoinjury,
refining
protocols
for
preserving
comprehensive
overview
underscores
transformative
potential
biomedicine,
while
emphasizing
necessity
innovation
address
existing
challenges.
Antioxidants,
Год журнала:
2024,
Номер
13(2), С. 247 - 247
Опубликована: Фев. 19, 2024
Cryopreservation
of
human
spermatozoa
is
a
necessity
for
males
suffering
from
infertility
who
cannot
produce
fresh
semen
insemination.
However,
current
ART
cryopreservation
protocols
are
associated
with
losses
sperm
motility,
vitality
and
DNA
integrity,
which
thought
to
be
linked
the
induction
oxidative
damage
toxic
properties
commercial
cryoprotectants
(CPAs).
Preventing
or
mitigating
these
would
hugely
beneficial
survival
during
ART.
Therefore,
in
this
vitro
investigation,
lipid
peroxidation,
production
reactive
oxygen
species,
movement
characteristics,
antioxidant
capacity,
vitality,
integrity
were
examined
samples
both
pre-
post-cryopreservation
CPA
supplementation.
The
findings
revealed
50%
reduction
capacity
addition,
was
accompanied
by
significant
increases
generation
species
formation
aldehydes.
These
changes
were,
turn,
correlated
reductions
viability,
motility
integrity.
Antioxidant
supplementation
generated
bell-shaped
dose-response
curves
resveratrol
vitamin
C,
emphasising
vulnerability
cells
reductive
stress.
At
optimal
dose,
C
able
significantly
enhance
reduce
recorded
cryopreserved
spermatozoa.
An
improvement
did
not
reach
statistical
significance,
possibly
because
additional
pathophysiological
mechanisms
limit
potential
effectiveness
antioxidants
rescuing
aspect
function.
stress
complex
nature
cryoinjury
will
present
major
challenges
creating
next
cryoprotective
media.
Journal of Assisted Reproduction and Genetics,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 29, 2025
Abstract
Purpose
To
create
a
novel
medium
that
retained
human
sperm
quality
following
cryopreservation
at
higher
level
than
seen
with
currently
available
commercial
cryoprotectants.
Methods
Cryopreservation
was
achieved
via
1:1
dilution
cryoprotectant
followed
by
slow-programmed
freezing.
A
NaCl-free
carrier
based
on
the
use
of
histidine
as
major
osmolyte
designed
capable
sustaining
motility
over
6
days
ambient
temperature.
This
supplemented
ethylene
glycol,
glycerol,
and
DMSO
to
basis
for
medium.
Dose-dependent
studies
various
supplements
were
then
conducted
optimize
effectiveness
this
formulation
including
assessments
vitamin
C,
EDTA,
crocin,
zinc,
ergothioneine,
myo
-inositol,
well
potential
replacement
Cyrene™.
Post-thaw
samples
assessed
motility,
vitality,
DNA
integrity
reassessed
isolation
Felix™
System.
Results
The
completed
comprised
4.5%
1%
in
0.4
mM
1
22
-inositol.
Spermatozoa
frozen
isolated
using
System
had
significantly
greater
total
progressive
spermatozoa
commercially
product
is
widely
used
infertility
clinics.
Conclusion
has
been
developed
study
represents
significant
improvement
existing
technologies.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(7), С. 4131 - 4131
Опубликована: Апрель 8, 2024
Sperm
cryopreservation
is
a
procedure
widely
used
to
store
gametes
for
later
use,
preserve
fertility
in
patients
prior
gonadotoxic
treatments
or
surgery,
and
sperm
donation
programs.
The
purpose
of
the
study
was
assess
impact
on
human
transcriptome.
Semen
samples
were
collected
from
13
normospermic
men.
Each
sample
divided
into
two
aliquots.
total
RNA
immediately
extracted
one
aliquot.
second
aliquot
frozen
after
week
storage
liquid
nitrogen.
randomized
four
pools,
each
six
donors,
analyzed
by
microarrays.
paired
Significance
Analysis
Microarray
performed.
We
found
219
lower
abundant
transcripts
28
higher
cryopreserved
than
fresh
sperm.
gene
ontology
analysis
disclosed
that
alters
pathways
important
(i.e.,
spermatogenesis,
motility,
mitochondria
function,
fertilization,
calcium
homeostasis,
cell
differentiation,
early
embryo
development),
although
increase
some
involved
immune
response
can
compensate
harmful
effects
freezing.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Май 29, 2024
Cryopreservation
of
sperm
can
cause
oxidative
stress
and
damage,
leading
to
decreased
different
functional
parameters
fertilization
potential.
In
this
study,
we
evaluated
two
types
H
