The Gut Microbiota Protects Bees from Invasion by a Bacterial Pathogen DOI Creative Commons
Margaret I. Steele, Erick V. S. Motta,

Tejashwini Gattu

и другие.

Microbiology Spectrum, Год журнала: 2021, Номер 9(2)

Опубликована: Сен. 15, 2021

Commensal microbes in animal guts often help to exclude bacterial pathogens. In honey bees, perturbing or depleting the gut microbiota increases host mortality rates upon challenge with opportunistic pathogen Serratia marcescens, suggesting antagonism between S. marcescens and one more members of bee microbiota. laboratory culture, uses a type VI secretion system (T6SS) kill competitors, but role this T6SS within hosts is unknown. Using infection assays, we determined how impacts abundance persistence visualized colocalization specific community situ. T6SS-deficient strains, measured T6SS-dependent killing isolates vitro compared mutant wild-type strains gut. We found that rapidly eliminated presence persists microbiota-free guts. Protection reduced monocolonized antibiotic-treated possibly because different symbionts occupy distinct niches. antagonize Escherichia coli other shows limited ability symbionts. Furthermore, achieved similar Thus, an intact offers robust protection against common pathogen, whose T6SSs do not confer compete commensal species. IMPORTANCE Bacteria living animals can provide by Some pathogens have been shown use molecular weapon known as beneficial bacteria during invasion mouse study, examined native work together marcescens. Although has bacteria, seem resistant its effects. This limitation may partially explain why ingestion rarely lethal insects healthy communities.

Язык: Английский

Host microbiota can facilitate pathogen infection DOI Creative Commons
Emily J. Stevens, Kieran A. Bates, Kayla C. King

и другие.

PLoS Pathogens, Год журнала: 2021, Номер 17(5), С. e1009514 - e1009514

Опубликована: Май 13, 2021

Animals live in symbiosis with numerous microbe species. While some can protect hosts from infection and benefit host health, components of the microbiota or changes to microbial landscape have potential facilitate infections worsen disease severity. Pathogens pathobionts exploit metabolites, take advantage a depletion defences changing conditions within host, cause opportunistic infection. The might also favour more virulent evolutionary trajectory for invading pathogens. In this review, we consider ways which contributes infectious throughout host’s life potentially across time. We further discuss implications these negative outcomes manipulation engineering management.

Язык: Английский

Процитировано

121
Glyphosate, but not its metabolite AMPA, alters the honeybee gut microbiota DOI Creative Commons
Nicolas Blot, Loïs Veillat,

Régis Rouzé

и другие.

PLoS ONE, Год журнала: 2019, Номер 14(4), С. e0215466 - e0215466

Опубликована: Апрель 16, 2019

The honeybee (Apis mellifera) has to cope with multiple environmental stressors, especially pesticides. Among those, the herbicide glyphosate and its main metabolite, aminomethylphosphonic acid (AMPA), are among most abundant ubiquitous contaminant in environment. Through foraging storing of contaminated resources, honeybees exposed these xenobiotics. As ingested AMPA directly contact gut microbiota, we used quantitative PCR test whether they could induce significant changes relative abundance major bacterial taxa. Glyphosate induced a strong decrease Snodgrassella alvi, partial Gilliamella apicola an increase Lactobacillus spp. abundances. In vitro, reduced growth S. alvi G. but not kunkeei. Although being no bee killer, confirmed that can have sublethal effects on microbiota. To such imbalanced microbiota favor pathogen development, were spores intestinal parasite Nosema ceranae. did significantly enhance effect infection. Concerning AMPA, while it reduce any change suggesting is active component modifying communities.

Язык: Английский

Процитировано

131
An Ecological Loop: Host Microbiomes across Multitrophic Interactions DOI
Hongwei Liu, Catriona A. Macdonald, James M. Cook

и другие.

Trends in Ecology & Evolution, Год журнала: 2019, Номер 34(12), С. 1118 - 1130

Опубликована: Авг. 15, 2019

Язык: Английский

Процитировано

124
Novel probiotic approach to counter Paenibacillus larvae infection in honey bees DOI Creative Commons
Brendan A. Daisley, Andrew P. Pitek,

John A. Chmiel

и другие.

The ISME Journal, Год журнала: 2019, Номер 14(2), С. 476 - 491

Опубликована: Окт. 29, 2019

Abstract American foulbrood (AFB) is a highly virulent disease afflicting honey bees (Apis mellifera). The causative organism, Paenibacillus larvae, attacks bee brood and renders entire hives dysfunctional during active states, but more commonly resides in asymptomatically as inactive spores that elude even vigilant beekeepers. mechanism of this pathogenic transition not fully understood, no cure exists for AFB. Here, we evaluated how hive supplementation with probiotic lactobacilli (delivered through nutrient patty; BioPatty) affected colony resistance towards naturally occurring AFB outbreak. Results demonstrated significantly lower pathogen load proteolytic activity larvae from BioPatty-treated hives. Interestingly, distinctive shift the microbiota composition adult nurse occurred irrespective treatment group monitoring period, only vehicle-supplemented exhibited higher P. loads. In vitro experiments utilizing laboratory-reared showed Lactobacillus plantarum Lp39, rhamnosus GR-1, kunkeei BR-1 (contained could reduce load, upregulate expression key immune genes, improve survival infection. These findings suggest usage lactobacilli-containing supplement, which practical affordable beekeepers, may be effective reducing enzootic pathogen-related losses.

Язык: Английский

Процитировано

121
New evidence showing that the destruction of gut bacteria by antibiotic treatment could increase the honey bee’s vulnerability to Nosema infection DOI Creative Commons

Jiang Hong Li,

Jay D. Evans, Wenfeng Li

и другие.

PLoS ONE, Год журнала: 2017, Номер 12(11), С. e0187505 - e0187505

Опубликована: Ноя. 10, 2017

It has become increasingly clear that gut bacteria play vital roles in the development, nutrition, immunity, and overall fitness of their eukaryotic hosts. We conducted present study to investigate effects microbiota disruption on honey bee's immune responses infection by microsporidian parasite Nosema ceranae. Newly emerged adult workers were collected divided into four groups: Group I-no treatment; II-inoculated with N. ceranae, III-antibiotic treatment, IV-antibiotic treatment after inoculation Our showed did not cause obvious bacterial community as there was no significant difference density composition between I II. However, elimination antibiotic (Groups III IV) negatively impacted functioning bees' system evidenced expression genes encoding antimicrobial peptides abaecin, defensin1, hymenoptaecin following ranking: > II IV. In addition, significantly higher levels observed IV than II, suggesting eliminating weakened function made bees more susceptible infection. Based having displayed highest mortality rate among experimental groups indicates combination stress, associated infection, impacts bee survival. The adds new evidence only leads complex problem resistance but can impact disease resistance. Further studies aimed at specific components will provide insights possibly approaches improving health.

Язык: Английский

Процитировано

120
Microbiome Structure Influences Infection by the Parasite Crithidia bombi in Bumble Bees DOI Open Access

Blair Mockler,

Waldan K. Kwong, Nancy A. Moran

и другие.

Applied and Environmental Microbiology, Год журнала: 2018, Номер 84(7)

Опубликована: Янв. 23, 2018

Recent declines in bumble bee populations are of great concern and have prompted critical evaluations the role pathogen introductions host resistance health. One factor that may influence resilience when facing infection is gut microbiota. Previous experiments with Bombus terrestris, a European bee, showed microbiota can protect against Crithidia bombi, widespread trypanosomatid parasite bees. However, particular characteristics microbiome responsible for this protective effect thus far eluded identification. Using wild commercially sourced impatiens, an important North American pollinator, we conducted cross-wise transplants to naive hosts both backgrounds challenged them parasite. As B. find microbiota-dependent protection operates impatiens Lower loads were experimentally associated high diversity, large bacterial populations, presence Apibacter, Lactobacillus Firm-5, Gilliamella spp. community. These results indicate even subtle differences between community structures significant impact on microbiome's ability defend infections.IMPORTANCE Many under threat due human activity, including through introduction pathogens via raised Recently, it was found help common parasite, but factors contributing unknown. bees, show total load, certain core members all susceptibility infection. Bee origin (genetic background) also factor. Finally, by examining phenomenon previously uninvestigated species, our study demonstrates microbiome-mediated conserved across multiple species. findings highlight how intricate interactions hosts, microbiomes, parasites wide-ranging consequences health ecologically

Язык: Английский

Процитировано

119
Honey bee gut dysbiosis: a novel context of disease ecology DOI Creative Commons
Kirk E. Anderson, Vincent Ricigliano

Current Opinion in Insect Science, Год журнала: 2017, Номер 22, С. 125 - 132

Опубликована: Июнь 27, 2017

Язык: Английский

Процитировано

116
Honey bee (Apis mellifera) gut microbiota promotes host endogenous detoxification capability via regulation of P450 gene expression in the digestive tract DOI Creative Commons
Yuqi Wu,

Yufei Zheng,

Yanan Chen

и другие.

Microbial Biotechnology, Год журнала: 2020, Номер 13(4), С. 1201 - 1212

Опубликована: Апрель 27, 2020

Summary There is growing number of studies demonstrating a close relationship between insect gut microbiota and insecticide resistance. However, the contribution honey bee to host detoxification ability has yet be investigated. In order address this question, we compared expression cytochrome P450s (P450s) genes deficient (GD) workers conventional community (CV) mortality rates pesticide residue levels GD CV treated with thiacloprid or tau ‐fluvalinate. Our results showed that promotes P450 enzymes in midgut, rate are significantly higher than those workers. Further comparisons tetracycline‐treated untreated demonstrated antibiotic‐induced dysbiosis leads attenuated midgut. The co‐treatment antibiotics pesticides reduced survival amount residues bees. Taken together, our symbiont could contribute health through modification xenobiotics pathways revealed potential negative impact health.

Язык: Английский

Процитировано

106
Missing Microbes in Bees: How Systematic Depletion of Key Symbionts Erodes Immunity DOI
Brendan A. Daisley,

John A. Chmiel,

Andrew P. Pitek

и другие.

Trends in Microbiology, Год журнала: 2020, Номер 28(12), С. 1010 - 1021

Опубликована: Июль 14, 2020

Язык: Английский

Процитировано

100
Understanding the Effects of Sublethal Pesticide Exposure on Honey Bees: A Role for Probiotics as Mediators of Environmental Stress DOI Creative Commons

John A. Chmiel,

Brendan A. Daisley, Andrew P. Pitek

и другие.

Frontiers in Ecology and Evolution, Год журнала: 2020, Номер 8

Опубликована: Фев. 18, 2020

Managed populations of the European honey bee (Apis mellifera) support production a global food supply. This important role in modern agriculture has rendered bees vulnerable to noxious effects anthropogenic stressors such as pesticides. Although deleterious outcomes lethal pesticide exposure on health and performance are apparent, ominous sublethal is an emerging concern well. Here, we use data harvesting approach better understand toxicological across life cycle. Through compiling adult- larval-specific median dose (LD50) values from 93 published sources, LD50 estimates for insecticides, herbicides, acaricides, fungicides highly variable studies, especially herbicides fungicides, which underrepresented meta-data set. Alongside major discrepancies these reported values, further examination compiled suggested that may not be ideal metric risk assessment. We also discuss how exposure, typically measured can diminish reproduction, immunity, cognition, overall physiological functioning, leading suboptimal population reduction. In consideration actionable solutions mitigate have identified potential probiotic supplementation promising strategy easily incorporated alongside current agricultural infrastructure apicultural management practices. Probiotic regularly employed apiculture but evidence-based targeted approaches yet been fully explored within formal context. benefits, practical considerations, limitations delivery probiotics hives. Ultimately, by subverting pesticides help improve long-term survival critical pollinators.

Язык: Английский

Процитировано

96