Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration

Bioactive Materials, Год журнала: 2021, Номер 9, С. 475 - 490

Опубликована: Июль 27, 2021

Sensory nerves promote osteogenesis through the release of neuropeptides. However, potential application and mechanism in which sensory healing bone defects presence biomaterials remain elusive. The present study identified that new formation was more abundantly produced after implantation silicified collagen scaffolds into created distal femur rats. wound sites were accompanied by extensive nerve innervation angiogenesis. dysfunction capsaicin injection resulted significant inhibition silicon-induced aforementioned rodent model. Application extracellular silicon vitro induced axon outgrowth increased expression semaphorin 3 A (Sema3A) 4D (Sema4D) dorsal root ganglion (DRG), as detected upregulation signaling molecules. Culture medium derived from silicon-stimulated DRG cells promoted proliferation differentiation marrow mesenchymal stem endothelial progenitor cells. These effects inhibited use Sema3A neutralizing antibodies but not Sema4D antibodies. Knockdown blocked angiogenesis almost completely a femoral defect rat model, whereas overexpression phenomena. Activation "mechanistic target rapamycin" (mTOR) pathway increase production rats implanted with scaffolds. findings support role inducing nerves, which, turn, stimulates Taken together, has therapeutic orthopedic rehabilitation.

Язык: Английский

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Skeletal interoception in bone homeostasis and pain

Cell Metabolism, Год журнала: 2022, Номер 34(12), С. 1914 - 1931

Опубликована: Окт. 17, 2022

Язык: Английский

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The “Three in One” Bone Repair Strategy for Osteoporotic Fractures

Frontiers in Endocrinology, Год журнала: 2022, Номер 13

Опубликована: Июнь 9, 2022

In aging society, osteoporotic fractures have become one major social problem threatening the health of elderly population in China. Compared with conventional fractures, low bone mass, defect and retarded healing issues lead to great difficulties treatment rehabilitation. Addressing concerns clinical settings, we proposed “three one” repair strategy focusing on anti-osteoporosis therapies, appropriate grafting fracture accelerating. We summarize misconceptions strategies for management, expecting improvement prognosis outcomes further improve therapeutic effect living quality patients.

Язык: Английский

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Mechanisms of cancer pain

Frontiers in Pain Research, Год журнала: 2023, Номер 3

Опубликована: Янв. 4, 2023

Personalised and targeted interventions have revolutionised cancer treatment dramatically improved survival rates in recent decades. Nonetheless, effective pain management remains a problem for patients diagnosed with cancer, who continue to suffer from the painful side effects of itself, as well treatments disease. This will grow an ageing population rapid advent more therapeutics treat Current guidelines World Health Organisation are generalised different severities, but fail address heterogeneity mechanisms varying types, stages disease plans. Pain is most common complaint leading emergency unit visits by over one-third that been experience under-treated pain. review summarises preclinical models states, particular focus on cancer-induced bone chemotherapy-associated We provide overview how can recapitulate aspects sensory dysfunction observed persistent or neuropathic following chemotherapy. Peripheral central nervous system discussed, along key cellular molecular mediators highlighted animal These include interactions between neuronal cells, cells non-neuronal tumour microenvironment. Therapeutic targets beyond opioid-based reviewed

Язык: Английский

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Calcium silicate nanowires-containing multicellular bioinks for 3D bioprinting of neural-bone constructs

Nano Today, Год журнала: 2022, Номер 46, С. 101584 - 101584

Опубликована: Авг. 9, 2022

Язык: Английский

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