The “Three in One” Bone Repair Strategy for Osteoporotic Fractures DOI Creative Commons
Xiao Chen, Yan Hu, Zhen Geng

et al.

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: June 9, 2022

In aging society, osteoporotic fractures have become one major social problem threatening the health of elderly population in China. Compared with conventional fractures, low bone mass, defect and retarded healing issues lead to great difficulties treatment rehabilitation. Addressing concerns clinical settings, we proposed “three one” repair strategy focusing on anti-osteoporosis therapies, appropriate grafting fracture accelerating. We summarize misconceptions strategies for management, expecting improvement prognosis outcomes further improve therapeutic effect living quality patients.

Language: Английский

Divalent metal cations stimulate skeleton interoception for new bone formation in mouse injury models DOI Creative Commons
Wei Qiao, Dayu Pan, Yufeng Zheng

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Jan. 27, 2022

Abstract Bone formation induced by divalent metal cations has been widely reported; however, the underlying mechanism is unclear. Here we report that these stimulate skeleton interoception promoting prostaglandin E2 secretion from macrophages. This immune response accompanied sprouting and arborization of calcitonin gene-related polypeptide-α + nerve fibers, which sense inflammatory cue with PGE 2 receptor 4 convey interoceptive signals to central nervous system. Activating downregulates sympathetic tone for new bone formation. Moreover, either macrophage depletion or knockout cyclooxygenase-2 in abolishes cation-induced interoception. Furthermore, sensory denervation EP4 nerves eliminates osteogenic effects cations. Thus, our study reveals promote through circuit, a finding could prompt development novel biomaterials elicit therapeutic power

Language: Английский

Citations

65
NGF-p75 signaling coordinates skeletal cell migration during bone repair DOI Creative Commons
Jiajia Xu, Zhao Li, Robert J. Tower

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(11)

Published: March 18, 2022

Bone regeneration following injury is initiated by inflammatory signals and occurs in association with infiltration sensory nerve fibers. Together, these events are believed to coordinate angiogenesis tissue reprogramming, but the mechanism of coupling immune reinnervation osteogenesis unknown. Here, we found that growth factor (NGF) expressed cranial bone via p75 resident mesenchymal osteogenic precursors affect their migration into damaged tissue. Mice lacking Ngf myeloid cells demonstrated reduced site consequently delayed healing. These features were phenocopied mice Pdgfra + osteoblast precursors. Single-cell transcriptomics identified subpopulations potential roles cell response, altered context deletion. results identify role signaling pathway coordinating skeletal during early repair.

Language: Английский

Citations

58
Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration DOI Creative Commons
Yuxuan Ma, Kai Jiao, Qianqian Wan

et al.

Bioactive Materials, Journal Year: 2021, Volume and Issue: 9, P. 475 - 490

Published: July 27, 2021

Sensory nerves promote osteogenesis through the release of neuropeptides. However, potential application and mechanism in which sensory healing bone defects presence biomaterials remain elusive. The present study identified that new formation was more abundantly produced after implantation silicified collagen scaffolds into created distal femur rats. wound sites were accompanied by extensive nerve innervation angiogenesis. dysfunction capsaicin injection resulted significant inhibition silicon-induced aforementioned rodent model. Application extracellular silicon vitro induced axon outgrowth increased expression semaphorin 3 A (Sema3A) 4D (Sema4D) dorsal root ganglion (DRG), as detected upregulation signaling molecules. Culture medium derived from silicon-stimulated DRG cells promoted proliferation differentiation marrow mesenchymal stem endothelial progenitor cells. These effects inhibited use Sema3A neutralizing antibodies but not Sema4D antibodies. Knockdown blocked angiogenesis almost completely a femoral defect rat model, whereas overexpression phenomena. Activation "mechanistic target rapamycin" (mTOR) pathway increase production rats implanted with scaffolds. findings support role inducing nerves, which, turn, stimulates Taken together, has therapeutic orthopedic rehabilitation.

Language: Английский

Citations

57
Skeletal interoception in bone homeostasis and pain DOI Creative Commons
Xiao Lv, Feng Gao, Xu Cao

et al.

Cell Metabolism, Journal Year: 2022, Volume and Issue: 34(12), P. 1914 - 1931

Published: Oct. 17, 2022

Language: Английский

Citations

55
The “Three in One” Bone Repair Strategy for Osteoporotic Fractures DOI Creative Commons
Xiao Chen, Yan Hu, Zhen Geng

et al.

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: June 9, 2022

In aging society, osteoporotic fractures have become one major social problem threatening the health of elderly population in China. Compared with conventional fractures, low bone mass, defect and retarded healing issues lead to great difficulties treatment rehabilitation. Addressing concerns clinical settings, we proposed “three one” repair strategy focusing on anti-osteoporosis therapies, appropriate grafting fracture accelerating. We summarize misconceptions strategies for management, expecting improvement prognosis outcomes further improve therapeutic effect living quality patients.

Language: Английский

Citations

48