The miR-9b microRNA mediates dimorphism and development of wing in aphids

Proceedings of the National Academy of Sciences, Год журнала: 2020, Номер 117(15), С. 8404 - 8409

Опубликована: Март 26, 2020

Wing dimorphism is a phenomenon of phenotypic plasticity in aphid dispersal. However, the signal transduction for perceiving environmental cues (e.g., crowding) and regulation mechanism remain elusive. Here, we found that aci-miR-9b was only down-regulated microRNA (miRNA) both crowding-induced wing during development brown citrus Aphis citricidus We determined targeted regulatory relationship between an ABC transporter (AcABCG4). Inhibition increased proportion winged offspring under normal conditions. Overexpression resulted decline crowding In addition, overexpression also malformed wings development. This role mediating confirmed pea Acyrthosiphon pisum The downstream action aci-miR-9b-AcABCG4 based on interaction with insulin insulin-like signaling pathway. A model demonstrated as following: maternal aphids experience crowding, which results decrease aci-miR-9b. followed by increase ABCG4, then activates pathway, thereby causing high offspring. Later, same cascade, "miR-9b-ABCG4-insulin signaling," again involved Taken together, our reveal cascade mediates via miRNA. These findings would be useful developing potential strategies blocking dispersal reducing viral transmission.

Язык: Английский

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Cas9-Mediated Gene-Editing in the Malaria Mosquito Anopheles stephensi by ReMOT Control

G3 Genes Genomes Genetics, Год журнала: 2020, Номер 10(4), С. 1353 - 1360

Опубликована: Март 2, 2020

Innovative tools are essential for advancing malaria control and depend on an understanding of molecular mechanisms governing transmission parasites by Anopheles mosquitoes. CRISPR/Cas9-based gene disruption is a powerful method to uncover underlying biology vector-pathogen interactions can itself form the basis mosquito strategies. However, embryo injection methods used genetically manipulate mosquitoes (especially ) difficult inefficient, particularly non-specialist laboratories. Here, we adapted ReMOT Control ( Re ceptor- m ediated O vary T ransduction C argo) technique deliver Cas9 ribonucleoprotein complex adult ovaries, generating targeted heritable mutations in vector stephensi without injecting embryos. In , editing was as efficient standard injections. The application opens power CRISPR/Cas9 laboratories that lack equipment or expertise perform injections establishes flexibility diverse species.

Язык: Английский

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Endocrine factors modulating vitellogenesis and oogenesis in insects: An update

Molecular and Cellular Endocrinology, Год журнала: 2024, Номер 587, С. 112211 - 112211

Опубликована: Март 15, 2024

Язык: Английский

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Pesticide-induced resurgence in brown planthopper is mediated by action on a suite of genes that promote juvenile hormone biosynthesis and female fecundity

Опубликована: Фев. 26, 2025

Pesticide-induced resurgence, increases in pest insect populations following pesticide application, is a serious threat to the sustainable control of many highly damaging crop pests. Resurgence can result from pesticide-enhanced reproduction, however, molecular mechanisms mediating this process remain unresolved. Here we show that brown planthopper (BPH) resurgence rice crops exposure emamectin benzoate (EB) results coordinated action suite genes regulate juvenile hormone (JH) levels, resulting increased JH titer adult females and enhanced fecundity. We demonstrate EB treatment at sublethal levels profound changes female BPH fitness including egg maturation oviposition. This reproductive EB-mediated upregulation key involved regulation JH, JHAMT Kr-h1 downregulation allatostatin ( AstA ) receptor AstAR expression. signaling known inhibit production corpora allata hence diminishes inhibitory action. find gene expression are caused by insecticide on its target, glutamate-gated chloride channel (GluCl). Collectively, these provide mechanistic insights into negative pesticide-induced responses insects reveal actors JH-signaling pathway underpin resurgence.

Язык: Английский

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Knockout of juvenile hormone receptor, Methoprene-tolerant, induces black larval phenotype in the yellow fever mosquito, Aedes aegypti

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 116(43), С. 21501 - 21507

Опубликована: Сен. 30, 2019

The yellow fever mosquito, Aedes aegypti, vectors human pathogens. Juvenile hormones (JH) control almost every aspect of an insect's life, and JH analogs are currently used to mosquito larvae. Since RNA interference does not work efficiently during the larval stages this insect, regulation development mode action well studied. To overcome limitation, we a multiple single guide RNA-based CRISPR/Cas9 genome-editing method knockout methoprene-tolerant (Met) gene coding for receptor. Met larvae exhibited black phenotype L3 (third instar larvae) L4 (fourth died before pupation. However, did affect embryonic or L1 L2 stages. Microscopy studies revealed precocious synthesis dark pupal cuticle Gene expression analysis showed that Krüppel homolog 1, key transcription factor in action, was down-regulated, but genes proteins involved melanization, adult synthesis, blood meal digestion adults were up-regulated mutants. These data suggest that, stages, mediates suppression pupal/adult melanization digestion. results help advance our knowledge Ae. aegypti.

Язык: Английский

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Cross-talk of insulin-like peptides, juvenile hormone, and 20-hydroxyecdysone in regulation of metabolism in the mosquito Aedes aegypti

Proceedings of the National Academy of Sciences, Год журнала: 2021, Номер 118(6)

Опубликована: Фев. 1, 2021

Significance In hematophagous female mosquitoes, each reproductive cycle is linked to a separate blood intake, serving as foundation for the transmission of dangerous human diseases. During cycle, mosquitoes sequentially feed on carbohydrates and protein (blood). Metabolic flux alternated support cyclicity. We have established that insulin-like peptides (ILPs), critical regulating metabolism, are genetically controlled by juvenile hormone (JH) 20-hydroxyecdysone (20E), key hormones governing reproduction mosquitoes. CRISPR gene-tagging experiments revealed JH 20E pathways coordinate production ILPs. This study has uncovered link between ILPs in controlling mosquito metabolism during Aedes aegypti mosquito.

Язык: Английский

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Endocrine Regulation of Lifespan in Insect Diapause

Frontiers in Physiology, Год журнала: 2022, Номер 13

Опубликована: Фев. 15, 2022

Diapause is a physiological adaptation to conditions that are unfavorable for growth or reproduction. During diapause, animals become long-lived, stress-resistant, developmentally static, and non-reproductive, in the case of diapausing adults. has been observed at all developmental stages both vertebrates invertebrates. In adults, diapause traits weaken into adaptations such as hibernation, estivation, dormancy, torpor, which represent evolutionarily diverse versions traditional traits. These regulated through modifications endocrine program guiding development. insects, this typically includes changes molting hormones, well metabolic signals limit while skewing organism’s energetic demands toward conservation. While much work done characterize these modifications, interactions between hormones their downstream consequences incompletely understood. The current state endocrinology reviewed here highlight relevance beyond its use model study seasonality Specifically, insect an emerging mechanisms determine lifespan. induction represents dramatic change normal progression age. Hormones juvenile hormone, 20-hydroxyecdysone, prothoracicotropic hormone well-known modulate plasticity. diapause—and by extension, cessation aging—is coordinated pathways. However, research directly connecting biology aging lacking. This review explores connections perspective signaling. fields suggests appreciable overlap will greatly contribute our understanding lifespan determination.

Язык: Английский

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Anopheline mosquitoes are protected against parasite infection by tryptophan catabolism in gut microbiota

Nature Microbiology, Год журнала: 2022, Номер 7(5), С. 707 - 715

Опубликована: Апрель 18, 2022

Язык: Английский

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Genome editing in pests: basic science to applications

Journal of Pest Science, Год журнала: 2024, Номер 97(3), С. 1135 - 1152

Опубликована: Янв. 24, 2024

Язык: Английский

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A specific type of insulin-like peptide regulates the conditional growth of a beetle weapon

PLoS Biology, Год журнала: 2019, Номер 17(11), С. e3000541 - e3000541

Опубликована: Ноя. 27, 2019

Evolutionarily conserved insulin/insulin-like growth factor (IGF) signaling (IIS) has been identified as a major physiological mechanism underlying the nutrient-dependent regulation of sexually selected weapon in animals. However, molecular mechanisms that couple nutritional state with remain largely unknown. Here, we show one specific subtype insulin-like peptide (ILP) responds to nutrient status and thereby regulates size broad-horned flour beetle Gnatocerus cornutus. By using transcriptome information, five G. cornutus ILP (GcorILP1–5) two receptor (GcorInR1, -2) genes genome. RNA interference (RNAi)-mediated gene silencing revealed certain ILP, GcorILP2, specifically regulated size. Importantly, GcorILP2 was highly expressed fat body condition-dependent manner. We further found GcorInR1 GcorInR2 are functionally redundant but latter is partially specialized for regulating growth. These results strongly suggest an important component developmental couples propose duplication subsequent diversification IIS played pivotal role evolution complex secondary sexual traits.

Язык: Английский

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