Wiley Interdisciplinary Reviews Developmental Biology,
Год журнала:
2020,
Номер
10(5)
Опубликована: Сен. 16, 2020
Abstract
Single‐cell
RNA
sequencing
(scRNA‐seq)
has
emerged
as
a
powerful
tool
for
investigating
cell
states
and
functions
at
the
single‐cell
level.
It
greatly
revolutionized
transcriptomic
studies
in
many
life
science
research
fields,
such
neurobiology,
immunology,
developmental
biology.
With
fast
development
of
both
experimental
platforms
bioinformatics
approaches
over
past
decade,
scRNA‐seq
is
becoming
economically
feasible
experimentally
practical
biomedical
laboratories.
Drosophila
served
an
excellent
model
organism
dissecting
cellular
molecular
mechanisms
that
underlie
tissue
development,
adult
function,
disease,
aging.
The
recent
application
methods
to
tissues
led
number
exciting
discoveries.
In
this
review,
I
will
provide
summary
,
focusing
on
technical
biological
applications.
also
discuss
current
challenges
future
opportunities
making
new
discoveries
using
.
This
article
categorized
under:
Technologies
>
Analysis
Transcriptome
Developmental Cell,
Год журнала:
2022,
Номер
57(10), С. 1271 - 1283.e4
Опубликована: Май 1, 2022
Drosophila
has
long
been
a
successful
model
organism
in
multiple
biomedical
fields.
Spatial
gene
expression
patterns
are
critical
for
the
understanding
of
complex
pathways
and
interactions,
whereas
temporal
changes
vital
studying
highly
dynamic
physiological
activities.
Systematic
studies
still
impeded
by
lack
spatiotemporal
transcriptomic
information.
Here,
utilizing
spatial
enhanced
resolution
omics-sequencing
(Stereo-seq),
we
dissected
developing
with
high
sensitivity.
We
demonstrated
that
Stereo-seq
data
can
be
used
3D
reconstruction
transcriptomes
embryos
larvae.
With
these
models,
identified
functional
subregions
embryonic
larval
midguts,
uncovered
cell
state
dynamics
testis,
revealed
known
potential
regulons
transcription
factors
within
their
topographic
background.
Our
provide
research
community
useful
resources
organism-wide
spatiotemporally
resolved
information
across
developmental
stages.
Cellular and Molecular Life Sciences,
Год журнала:
2020,
Номер
77(22), С. 4523 - 4551
Опубликована: Май 24, 2020
Organisms
adapt
to
changing
environments
by
adjusting
their
development,
metabolism,
and
behavior
improve
chances
of
survival
reproduction.
To
achieve
such
flexibility,
organisms
must
be
able
sense
respond
changes
in
external
environmental
conditions
internal
state.
Metabolic
adaptation
response
altered
nutrient
availability
is
key
maintaining
energy
homeostasis
sustaining
developmental
growth.
Furthermore,
variables
exert
major
influences
on
growth
final
adult
body
size
animals.
This
plasticity
depends
adaptive
responses
state
cues
that
are
essential
for
processes.
Genetic
studies
have
shown
the
fruit
fly
Drosophila,
similarly
mammals,
regulates
its
growth,
environment
through
several
hormones
including
insulin,
peptides
with
glucagon-like
function,
steroid
hormones.
Here
we
review
emerging
evidence
showing
various
sensed
different
organs
that,
via
inter-organ
communication,
relay
information
neuroendocrine
centers
control
insulin
signaling.
focuses
endocrine
regulation
highlighting
recent
advances
role
system
as
a
signaling
hub
integrates
inputs
drives
responses.
Nature Communications,
Год журнала:
2021,
Номер
12(1)
Опубликована: Авг. 10, 2021
Abstract
The
enteroendocrine
cell
(EEC)-derived
incretins
play
a
pivotal
role
in
regulating
the
secretion
of
glucagon
and
insulins
mammals.
Although
glucagon-like
insulin-like
hormones
have
been
found
across
animal
phyla,
incretin-like
EEC-derived
not
yet
characterised
invertebrates.
Here,
we
show
that
midgut-derived
hormone,
neuropeptide
F
(NPF),
acts
as
sugar-responsive,
hormone
fruit
fly,
Drosophila
melanogaster
.
Secreted
NPF
is
received
by
receptor
corpora
cardiaca
insulin-producing
cells.
NPF-NPFR
signalling
resulted
suppression
production
enhancement
peptide
secretion,
eventually
promoting
lipid
anabolism.
Similar
to
loss
incretin
function
mammals,
midgut
led
significant
metabolic
dysfunction,
accompanied
lipodystrophy,
hyperphagia,
hypoglycaemia.
These
results
suggest
regulate
sugar-dependent
metabolism
through
only
mammals
but
also
insects.
Nucleic Acids Research,
Год журнала:
2021,
Номер
50(D1), С. D1010 - D1015
Опубликована: Окт. 8, 2021
Abstract
FlyAtlas
2
(flyatlas2.org)
is
a
database
and
web
application
for
studying
the
expression
of
genes
Drosophila
melanogaster
in
different
tissues
adults
larvae.
It
based
on
RNA-Seq
data,
incorporates
both
encoding
proteins
microRNAs.
We
have
now
completed
population
with
13
from
male
female
adults,
five
sex-specific
tissues,
eight
larval
tissues.
Larval
garland
cell
nephrocytes
also
been
included.
Major
enhancements
made
to
application.
First,
facility
has
added
‘Profile’
search
similar
pattern
tissue
as
query
gene.
This
may
help
establish
function
which
this
currently
unknown.
Second,
dedicated
midgut,
where
difference
gene
regions
pH
can
be
explored.
A
variety
further
improvements
interface
are
described.
Drosophila
melanogaster
is
a
powerful,
long-standing
model
for
metazoan
development
and
gene
regulation.
We
profiled
chromatin
accessibility
in
almost
1
million
expression
half
nuclei
from
overlapping
windows
spanning
the
entirety
of
embryogenesis.
Leveraging
developmental
asynchronicity
within
embryo
collections,
we
applied
deep
neural
networks
to
infer
age
each
nucleus,
resulting
continuous,
multimodal
views
molecular
cellular
transitions
absolute
time.
identify
cell
lineages;
their
relationships;
link
dynamic
changes
enhancer
usage,
transcription
factor
(TF)
expression,
TFs’
cognate
motifs.
With
these
data,
dynamics
usage
can
be
explored
across
lineages
at
scale
minutes,
including
precise
like
zygotic
genome
activation.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2021,
Номер
unknown
Опубликована: Июль 5, 2021
Abstract
The
ability
to
obtain
single
cell
transcriptomes
for
stable
types
and
dynamic
states
is
ushering
in
a
new
era
biology.
We
created
the
Tabula
Drosophilae
,
atlas
of
adult
fruit
fly
which
includes
580k
cells
from
15
individually
dissected
sexed
tissues
as
well
entire
head
body.
Over
100
researchers
community
contributed
annotations
>250
distinct
across
all
tissues.
provide
an
in-depth
analysis
type-related
gene
signatures
transcription
factor
markers,
sexual
dimorphism,
whole
animal.
Analysis
common
that
are
shared
between
tissues,
such
blood
muscle
cells,
allowed
discovery
rare
tissue-specific
subtypes.
This
provides
valuable
resource
Drosophila
serves
comprehensive
reference
study
genetic
perturbations
disease
models
at
resolution.
