CANDIDATUS LIST No. 3. Lists of names of prokaryotic Candidatus taxa

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Год журнала: 2022, Номер 72(1)

Опубликована: Янв. 31, 2022

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher we support invest in the microbiology community, to benefit of everyone. This supports our principal goal develop, expand strengthen networks available members so that they can generate new knowledge about microbes ensure it is shared with other communities.

Язык: Английский

---

The Genomics and Cell Biology of Host-Beneficial Intracellular Infections

Annual Review of Cell and Developmental Biology, Год журнала: 2021, Номер 37(1), С. 115 - 142

Опубликована: Июль 9, 2021

Microbes gain access to eukaryotic cells as food for bacteria-grazing protists, host protection by microbe-killing immune cells, or microbial benefit when pathogens enter replicate. But microbes can also a cell and become an important-often required-beneficial partner. The oldest beneficial infections are the ancient organelles now called mitochondrion plastid. numerous other host-beneficial intracellular occur throughout eukaryotes. Here I review genomics biology of these interactions with focus on bacteria. genomes bacteria have features that span previously unfilled gap between organelles. Host adaptations allow persistence found along evidence manipulation but cellular mechanisms bacterial not well understood.

Язык: Английский

---

Till evolution do us part: The diversity of symbiotic associations across populations of Philaenus spittlebugs

Environmental Microbiology, Год журнала: 2023, Номер 25(11), С. 2431 - 2446

Опубликована: Авг. 1, 2023

Symbiotic bacteria have played crucial roles in the evolution of sap-feeding insects and can strongly affect host function. However, their diversity distribution within species are not well understood; we do know to what extent environmental factors or associations with other may microbial community profiles. We addressed this question Philaenus spittlebugs by surveying both insect bacterial marker gene amplicons across multiple populations. Host mitochondrial sequence data confirmed morphology-based identification six revealed two divergent clades spumarius. All them hosted primary symbiont Sulcia that was almost always accompanied Sodalis. Interestingly, populations individuals often differed presence Sodalis variants, suggestive intra-genome 16S rRNA variant polymorphism combined rapid genome and/or recent additional infections replacements co-primary symbiont. The prevalence facultative endosymbionts, including Wolbachia, Rickettsia, Spiroplasma, varied among Notably, cytochrome I oxidase (COI) amplicon also showed nearly a quarter P. spumarius were infected parasitoid flies (Verralia aucta). One Wolbachia operational taxonomic units (OTUs) exclusively present Verralia-parasitized specimens, parasitoids as source highlighting utility sequencing microbiome studies.

Язык: Английский

---

Exposure to Trypanosoma parasites induces changes in the microbiome of the Chagas disease vector Rhodnius prolixus

Microbiome, Год журнала: 2022, Номер 10(1)

Опубликована: Март 10, 2022

Abstract Background The causative agent of Chagas disease, Trypanosoma cruzi , and its nonpathogenic relative, rangeli are transmitted by haematophagous triatomines undergo a crucial ontogenetic phase in the insect’s intestine. In process, parasites interfere with host immune system as well microbiome present digestive tract potentially establishing an environment advantageous for development. However, coherent interactions between host, pathogen microbiota have not yet been elucidated detail. We applied metagenome shotgun sequencing approach to study alterations Rhodnius prolixus major vector after exposure T. focusing also on functional capacities intestinal insect. Results R. was dominated bacterial orders Enterobacterales Corynebacteriales Lactobacillales Clostridiales Chlamydiales whereas latter conceivably originated from blood used exposure. anterior posterior midgut samples exposed insects showed reduced overall number organisms compared control group. we found enriched groups T . While relative abundance decreased considerably, mainly composed genus Enterococcus developed most abundant taxonomic This applies particular vectors challenged at early timepoints Furthermore, were able reconstruct four metagenome-assembled genomes elucidate their unique metabolic functionalities within triatomine microbiome, including genome recently described insect symbiont, Candidatus Symbiopectobacterium secondary metabolites producing bacteria Kocuria spp. Conclusions Our results facilitate deeper understanding processes that take place during colonisation trypanosomal highlight influential aspects pathogen-microbiota interactions. particular, mostly unexplored vector’s clearer, underlining role transmission disease.

Язык: Английский

---

Eco-evolutionary implications of helminth microbiomes

Journal of Helminthology, Год журнала: 2023, Номер 97

Опубликована: Янв. 1, 2023

Abstract The evolution of helminth parasites has long been seen as an interplay between host resistance to infection and the parasite's capacity bypass such resistance. However, there recently increasing appreciation role symbiotic microbes in interaction their hosts. It is now clear that helminths have a different microbiome from organisms they parasitize, sometimes amid large variability, components are shared among life stages or populations parasite. Helminths shown acquire parent generations (vertical transmission) surroundings (horizontal transmission). In this latter case, natural selection strongly linked fact helminth-associated microbiota not simply random assemblage pool available organismal hosts environments. Indeed, some specific microbial taxa evolved complex ecological relationships, ranging obligate mutualism reproductive manipulation by associated microbes. our understanding still very elementary regarding net effect all eco-evolution with non-exhaustible review, we focus on bacterial (as opposed hosts) highlight relevant concepts key findings transmission, associations, taxonomic functional diversity bacteriome. We integrate dimension discussion identify fundamental knowledge gaps, finally suggesting research avenues for eco-evolutionary impacts host–parasite interactions light new technological developments.

Язык: Английский

---

Protective role of native root-associated bacterial consortium against root-knot nematode infection in susceptible plants

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 7, 2024

Root-knot nematodes (RKNs) are a global menace to agricultural crop production. The role of root-associated microbes (RAMs) in plant protection against RKN infection remains unclear. Here we observe that cucumber (highly susceptible Meloidogyne incognita) exhibits consistently lower susceptibility M. incognita the presence native RAMs three distinct soils. Nematode alters assembly bacterial along life cycle incognita. Particularly, loss diversity exacerbates A diverse range strains isolated from incognita-infected roots has nematode-antagonistic activity. Increasing number causes decreasing nematode infection, which is lowest when six or more present. Multiple simplified synthetic communities consisting show pronounced inhibitory effects on plants. These underpinned via multiple mechanisms including direct inhibition secretion anti-nematode substances, and regulation defense responses. This study highlights resistance RKNs provides useful insight into development sustainable way protect soil-borne parasites damage most Here, authors consortium exhibit general suppression infections by offering protective functions.

Язык: Английский

---

Prevalence of Heritable Symbionts in Parisian Bedbugs (Hemiptera: Cimicidae)

Environmental Microbiology Reports, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 29, 2025

ABSTRACT Like many insects, the biology of bedbugs is impacted by a range partner heritable microbes. Three maternally inherited symbionts are recognised: Wolbachia (an obligate partner), Symbiopectobacterium purcellii strain Sy Clec, and Candidatus Tisiphia sp. (facultative typically present in some but not all individuals). Past work had examined presence these microbes from established laboratory lines, broader field samples. We therefore deployed targeted endpoint PCR assays to determine symbiont infection status for 50 collected 10 districts Paris during 2023 outbreak. All three were found be broadly across Cimex lectularius samples, with ‐ Tisiphia‐ triple most commonly observed. A minority individuals lacked either one or both facultative symbionts. Five mtDNA haplotypes observed COI barcode region, infections haplotypes, indicating that recent invasion event. conclude Parisian bedbug outbreak was which host's secondary at high‐frequency coinfections, an important uncharacterised component populations.

Язык: Английский

---

Does the host matter? Testing the impact of host identity on the microbiome of a trematode parasite

Parasitology Research, Год журнала: 2025, Номер 124(3)

Опубликована: Март 1, 2025

Язык: Английский

---

Symbiopectobacterium purcellii, gen. nov., sp. nov., isolated from the leafhopper Empoasca decipiens

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Год журнала: 2022, Номер 72(6)

Опубликована: Июнь 9, 2022

Bacterial endosymbionts are found in multiple arthropod species, where they play crucial roles as nutritional symbionts, defensive symbionts or reproductive parasites. Recent work has highlighted a new clade of heritable microbes within the gammaproteobacteria that enter into both obligate and facultative symbioses, with an obligately required unculturable symbiont recently given name Candidatus Symbiopectobacterium. In this study, we describe culturable rod shaped non-flagellated bacterial from isolated leafhopper Empoasca decipiens. The is related to transovarially transmitted 'BEV' bacterium was first Euscelidius variegatus by Alexander Purcell, therefore Symbiopectobacterium purcellii sp. nov., gen. nov. We further report closed genome sequence for S. purcellii. atypical microbe, being large size, without profound AT bias little evidence pseudogenization. predicted encode Type II, III VI secretion systems associated effectors non-ribosomal peptide synthase array likely produce bioactive small molecules. metabolism more complete than other clade, microbe synthesize range B vitamins. However, Biolog plate results indicate depauperate compared sister represented Pectobacterium carotovorum. A quorum-sensing pathway species (containing overlapping expI-expR1 pair opposite directions "solo" expR2) evidenced, LC-MS/MS analysis reveals presence 3-hydroxy-C10-HSL sole N-acylhomoserine lactone (AHL) our strain. This AHL profile profoundly divergent Erwinia which mostly 3-oxo-C6- 3-oxo-C8-HSL could aid group identification. Thus, denotes one lost certain pathways saprophytic lifestyle but represents important baseline against compare members genus show integration host biology. type strain SyEd1T (LMG 32449T=CECT 30436T).

Язык: Английский

---

The Evolution of Interdependence in a Four-Way Mealybug Symbiosis

Genome Biology and Evolution, Год журнала: 2021, Номер 13(8)

Опубликована: Май 31, 2021

Abstract Mealybugs are insects that maintain intracellular bacterial symbionts to supplement their nutrient-poor plant sap diets. Some mealybugs have a single betaproteobacterial endosymbiont, Candidatus Tremblaya species (hereafter Tremblaya) alone provides the insect with its required nutrients. Other two nutritional endosymbionts together provision these same nutrients, where has gained gammaproteobacterial partner resides in cytoplasm. Previous work had established Pseudococcus longispinus not one but of along Tremblaya. Preliminary genomic analyses suggested large genomes features consistent relatively recent origin as endosymbionts, patterns complementarity between members symbiosis and relative cellular locations were unknown. Here, using long-read sequencing various types microscopy, we show P. mixed within cells, somewhat reduced size compared closest nonendosymbiotic relatives. Both contain thousands pseudogenes, shift from free-living an endosymbiotic lifestyle. Biosynthetic pathways key metabolites partitioned complex interdependent among genomes, genome, horizontally acquired genes encoded on mealybug nuclear genome. Although been recently evolutionary time, they already evolved codependencies each other, Tremblaya, host.

Язык: Английский

---