Genome-Wide Identification of the CIF Gene Family and Protein Interaction with GSO1s Under the p-HBA-Induced Continuous Cropping Obstacle in Pogostemon cablin

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1568 - 1568

Опубликована: Фев. 13, 2025

Casparian strip integrity factors (CIFs), which are tyrosine-sulfated small peptides, crucial genes involved in the formation and regulation of play an important role plant stress response. In order to explore evolution, characteristics, role, function CIFs response continuous cropping obstacles (CCOs), bioinformatics gene expression analysis CIF Pogostemon cablin was carried out by determining phylogenetic relationship, chromosome location, structure, RT-qPCR results. Results showed that a total 12 PatCIF family were identified on different chromosomes. Promoter prediction revealed 16 cis-regulatory elements. A systematic evolutionary study 33 species indicates originated from Spermatophyta. Collinearity P. shared 19 syntenic with Solanum lycopersicum only 8 Oryza sativa. Transcriptome indicated PatCIF1-4 PatGSO1b/1c/1f decreased under p-hydroxybenzoic acid treatment, further validation four consistent AlphaFold protein interaction region between mature peptide via LRR domain, provides key binding surface for PatCIFs. This offers theoretical basis investigate roles PatCIFs PatGSO1s CCOs their interactions cablin.

Язык: Английский

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Peptides in plant reproduction—small yet powerful

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Фев. 24, 2025

Peptides, currently being considered as a novel class of plant hormones, play diverse roles in growth and development. Peptides trigger signaling by binding to receptors co-receptors, thus activating cellular pathways. Recently, peptides have been extensively investigated reproduction-related processes, ranging from gametes development fusion. In this review, we summarize recent advancements related the role reproduction discuss challenges that need be addressed urgently.

Язык: Английский

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Identification of the Casparian strip integrity factor (CIF) gene family in Brassica napus and functional prediction of mature CIF small peptides

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Июнь 5, 2025

Small peptides of the CIF family act as signaling molecules and participate in important pathways, playing crucial roles processes plant development environmental response. However, has not been identified allopolyploid Brassica napus. This study 16 BnCIFs coding genes napus, classified into three phylogenetic subfamilies four types (CIF1/2, CIF3, CIF4, TWS1) based on Arabidopsis thaliana homologs. Chromosomal distribution analysis revealed their even spread across 11 chromosomes, primarily inherited from rapa with evolutionary contributions chromosomal segment duplication, recombination, polyploidization. Conserved features were observed mature (21/23 amino acids), including an N-terminal DY motif, two central prolines, hydrophilic C-terminal residues. Promoter regions contained multiple hormone- stress-responsive cis-elements, suggesting diverse regulatory processes. Spatiotemporal expression profiling demonstrated tissue-specific patterns within each BnCIF type, alongside broad responsiveness to exogenous hormones abiotic stress, implying functional diversification. qRT-PCR CIF1/2-type under nitrogen treatments significant upregulation roots low nitrogen, indicating potential involvement absorption/transport. Comparative structural GSO1/2 receptor kinases between napus suggested possible BnCIFs-GSO1/2 binding interactions. Our comprehensively aspects such gene structure, phylogeny, location, duplication patterns, characteristics, conservation small after cleavage processing, ability bind receptors. The results lay a foundation for further BnCIFs. In further, deciphering network may provide new targets optimizing root architecture breeding stress resistance

Язык: Английский

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A systematic analysis of the subtilase gene family and expression and subcellular localization investigation of anther-specific members in maize

Plant Physiology and Biochemistry, Год журнала: 2023, Номер 203, С. 108041 - 108041

Опубликована: Сен. 15, 2023

Язык: Английский

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The cellulose synthase-like gene CaCslD1 is required for male fertility in pepper

Scientia Horticulturae, Год журнала: 2024, Номер 337, С. 113491 - 113491

Опубликована: Июль 19, 2024

Язык: Английский

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Imaging the spatial distribution of structurally diverse plant hormones: A comprehensive review

Journal of Experimental Botany, Год журнала: 2024, Номер 75(22), С. 6980 - 6997

Опубликована: Сен. 12, 2024

Abstract Plant hormones are essential and structurally diverse molecules that regulate various aspects of plant growth, development, stress responses. However, the precise analysis in complex biological samples poses a challenge due to their low concentrations, dynamic levels, intricate spatial distribution. Moreover, complexity interconnectedness hormone signaling networks make it difficult simultaneously trace multiple distributions. In this review, we provide an overview currently recognized small-molecule hormones, signal peptide growth regulators, along with analytical methods employed for analysis. We delve into latest advancements mass spectrometry imaging situ fluorescence techniques, which enable examination distribution hormones. The advantages disadvantages these techniques further discussed. Finally, propose potential avenues enhance our understanding biology.

Язык: Английский

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Tyrosylprotein Sulfotransferase Positively Regulates Symbiotic Nodulation and Root Growth

Plant Cell & Environment, Год журнала: 2024, Номер unknown

Опубликована: Сен. 17, 2024

ABSTRACT Posttranslational tyrosine sulfation of peptides and proteins is catalysed by tyrosylprotein sulfotransferases (TPSTs). In Arabidopsis , essential for the activities peptide hormones, such as phytosulfokine (PSK) root meristem growth factor (RGF). Here, we identified a TPST‐encoding gene, MtTPST from model legume Medicago truncatula . expression was detected in all organs, with highest level nodules. A promoter:GUS assay revealed that highly expressed apical meristem, nodule primordium meristem. The loss‐of‐function mutant mttpst exhibited stunted phenotype short roots reduced number size. Application both sulfated PSK RGF3 partially restored defective length reduction symbiotic nodulation recovered treatment peptide. MtTPST‐PSK module functions downstream Nod signalling to promote initiation via regulating accumulation and/or cytokinin auxin. Additionally, small‐nodule which resulted decreased meristematic activity, complemented treatment. Together, these results demonstrate MtTPST, through its substrates PSK, other peptide(s), positively regulates development growth.

Язык: Английский

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Anther development in Arabidopsis thaliana involves symplastic isolation and apoplastic gating of the tapetum-middle layer interface

Development, Год журнала: 2022, Номер 149(22)

Опубликована: Окт. 28, 2022

During flowering plant reproduction, anthers produce pollen grains, the development of which is supported by tapetum, a nourishing maternal tissue that also contributes non-cell-autonomously to wall, resistant external layer on surface. How anther restricts movement tapetum-derived wall components, while allowing metabolites such as sugars and amino acids reach developing pollen, remains unknown. Here, we show experimentally in arabidopsis thaliana tapetum are symplastically isolated from each other, other sporophytic tissues, meiosis onwards. We peritapetal strip, an apoplastic structure, separates grains cell layers can prevent diffusion fluorescent proteins, again The formation selective barrier functions strip require two NADPH oxidases, RBOHE RBOHC, play key role formation. Our results suggest that, together with symplastic isolation, gating apoplast around may help generate metabolically distinct compartments.

Язык: Английский

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A Rapid Alkalinization Factor-like Peptide EaF82 Impairs Tapetum Degeneration during Pollen Development through Induced ATP Deficiency

Cells, Год журнала: 2023, Номер 12(11), С. 1542 - 1542

Опубликована: Июнь 4, 2023

In plants, the timely degeneration of tapetal cells is essential for providing nutrients and other substances to support pollen development. Rapid alkalinization factors (RALFs) are small, cysteine-rich peptides known be involved in various aspects plant development growth, as well defense against biotic abiotic stresses. However, functions most them remain unknown, while no RALF has been reported involve tapetum degeneration. this study, we demonstrated that a novel peptide, EaF82, isolated from shy-flowering ‘Golden Pothos’ (Epipremnum aureum) RALF-like peptide displays alkalinizing activity. Its heterologous expression Arabidopsis delayed reduced production seed yields. RNAseq, RT-qPCR, biochemical analyses showed overexpression EaF82 downregulated group genes pH changes, cell wall modifications, degeneration, maturation, seven endogenous genes, decreased proteasome activity ATP levels. Yeast two-hybrid screening identified AKIN10, subunit energy-sensing SnRK1 kinase, its interacting partner. Our study reveals possible regulatory role suggests action may mediated through AKIN10 leading alteration transcriptome energy metabolism, thereby causing deficiency impairing

Язык: Английский

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Current Advances in Small Peptides During Plant Growth and Development

Опубликована: Июнь 4, 2024

Small peptides (SPs), ranging from 5 to 100 amino acids, play integral roles in plants due their diverse functions. Despite low abundance and small molecular weight, SPs intricately regulate critical aspects of plant life, including cell division, growth, differentiation, flowering, fruiting, maturation, stress responses. As vital mediators intercellular signaling, have garnered significant attention biology research. This comprehensive review delves into SPs' structure, classification, identification, providing a detailed understanding significance. Additionally, we summarize recent findings on the biological functions signaling pathways prominent that growth development. The also offers forward-looking perspective future research directions peptide pathways.

Язык: Английский

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