International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(4), С. 1568 - 1568
Опубликована: Фев. 13, 2025
Casparian
strip
integrity
factors
(CIFs),
which
are
tyrosine-sulfated
small
peptides,
crucial
genes
involved
in
the
formation
and
regulation
of
play
an
important
role
plant
stress
response.
In
order
to
explore
evolution,
characteristics,
role,
function
CIFs
response
continuous
cropping
obstacles
(CCOs),
bioinformatics
gene
expression
analysis
CIF
Pogostemon
cablin
was
carried
out
by
determining
phylogenetic
relationship,
chromosome
location,
structure,
RT-qPCR
results.
Results
showed
that
a
total
12
PatCIF
family
were
identified
on
different
chromosomes.
Promoter
prediction
revealed
16
cis-regulatory
elements.
A
systematic
evolutionary
study
33
species
indicates
originated
from
Spermatophyta.
Collinearity
P.
shared
19
syntenic
with
Solanum
lycopersicum
only
8
Oryza
sativa.
Transcriptome
indicated
PatCIF1-4
PatGSO1b/1c/1f
decreased
under
p-hydroxybenzoic
acid
treatment,
further
validation
four
consistent
AlphaFold
protein
interaction
region
between
mature
peptide
via
LRR
domain,
provides
key
binding
surface
for
PatCIFs.
This
offers
theoretical
basis
investigate
roles
PatCIFs
PatGSO1s
CCOs
their
interactions
cablin.
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 24, 2025
Peptides,
currently
being
considered
as
a
novel
class
of
plant
hormones,
play
diverse
roles
in
growth
and
development.
Peptides
trigger
signaling
by
binding
to
receptors
co-receptors,
thus
activating
cellular
pathways.
Recently,
peptides
have
been
extensively
investigated
reproduction-related
processes,
ranging
from
gametes
development
fusion.
In
this
review,
we
summarize
recent
advancements
related
the
role
reproduction
discuss
challenges
that
need
be
addressed
urgently.
Journal of Experimental Botany,
Год журнала:
2024,
Номер
75(22), С. 6980 - 6997
Опубликована: Сен. 12, 2024
Abstract
Plant
hormones
are
essential
and
structurally
diverse
molecules
that
regulate
various
aspects
of
plant
growth,
development,
stress
responses.
However,
the
precise
analysis
in
complex
biological
samples
poses
a
challenge
due
to
their
low
concentrations,
dynamic
levels,
intricate
spatial
distribution.
Moreover,
complexity
interconnectedness
hormone
signaling
networks
make
it
difficult
simultaneously
trace
multiple
distributions.
In
this
review,
we
provide
an
overview
currently
recognized
small-molecule
hormones,
signal
peptide
growth
regulators,
along
with
analytical
methods
employed
for
analysis.
We
delve
into
latest
advancements
mass
spectrometry
imaging
situ
fluorescence
techniques,
which
enable
examination
distribution
hormones.
The
advantages
disadvantages
these
techniques
further
discussed.
Finally,
propose
potential
avenues
enhance
our
understanding
biology.
Plant Cell & Environment,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 17, 2024
ABSTRACT
Posttranslational
tyrosine
sulfation
of
peptides
and
proteins
is
catalysed
by
tyrosylprotein
sulfotransferases
(TPSTs).
In
Arabidopsis
,
essential
for
the
activities
peptide
hormones,
such
as
phytosulfokine
(PSK)
root
meristem
growth
factor
(RGF).
Here,
we
identified
a
TPST‐encoding
gene,
MtTPST
from
model
legume
Medicago
truncatula
.
expression
was
detected
in
all
organs,
with
highest
level
nodules.
A
promoter:GUS
assay
revealed
that
highly
expressed
apical
meristem,
nodule
primordium
meristem.
The
loss‐of‐function
mutant
mttpst
exhibited
stunted
phenotype
short
roots
reduced
number
size.
Application
both
sulfated
PSK
RGF3
partially
restored
defective
length
reduction
symbiotic
nodulation
recovered
treatment
peptide.
MtTPST‐PSK
module
functions
downstream
Nod
signalling
to
promote
initiation
via
regulating
accumulation
and/or
cytokinin
auxin.
Additionally,
small‐nodule
which
resulted
decreased
meristematic
activity,
complemented
treatment.
Together,
these
results
demonstrate
MtTPST,
through
its
substrates
PSK,
other
peptide(s),
positively
regulates
development
growth.
During
flowering
plant
reproduction,
anthers
produce
pollen
grains,
the
development
of
which
is
supported
by
tapetum,
a
nourishing
maternal
tissue
that
also
contributes
non-cell-autonomously
to
wall,
resistant
external
layer
on
surface.
How
anther
restricts
movement
tapetum-derived
wall
components,
while
allowing
metabolites
such
as
sugars
and
amino
acids
reach
developing
pollen,
remains
unknown.
Here,
we
show
experimentally
in
arabidopsis
thaliana
tapetum
are
symplastically
isolated
from
each
other,
other
sporophytic
tissues,
meiosis
onwards.
We
peritapetal
strip,
an
apoplastic
structure,
separates
grains
cell
layers
can
prevent
diffusion
fluorescent
proteins,
again
The
formation
selective
barrier
functions
strip
require
two
NADPH
oxidases,
RBOHE
RBOHC,
play
key
role
formation.
Our
results
suggest
that,
together
with
symplastic
isolation,
gating
apoplast
around
may
help
generate
metabolically
distinct
compartments.
Cells,
Год журнала:
2023,
Номер
12(11), С. 1542 - 1542
Опубликована: Июнь 4, 2023
In
plants,
the
timely
degeneration
of
tapetal
cells
is
essential
for
providing
nutrients
and
other
substances
to
support
pollen
development.
Rapid
alkalinization
factors
(RALFs)
are
small,
cysteine-rich
peptides
known
be
involved
in
various
aspects
plant
development
growth,
as
well
defense
against
biotic
abiotic
stresses.
However,
functions
most
them
remain
unknown,
while
no
RALF
has
been
reported
involve
tapetum
degeneration.
this
study,
we
demonstrated
that
a
novel
peptide,
EaF82,
isolated
from
shy-flowering
‘Golden
Pothos’
(Epipremnum
aureum)
RALF-like
peptide
displays
alkalinizing
activity.
Its
heterologous
expression
Arabidopsis
delayed
reduced
production
seed
yields.
RNAseq,
RT-qPCR,
biochemical
analyses
showed
overexpression
EaF82
downregulated
group
genes
pH
changes,
cell
wall
modifications,
degeneration,
maturation,
seven
endogenous
genes,
decreased
proteasome
activity
ATP
levels.
Yeast
two-hybrid
screening
identified
AKIN10,
subunit
energy-sensing
SnRK1
kinase,
its
interacting
partner.
Our
study
reveals
possible
regulatory
role
suggests
action
may
mediated
through
AKIN10
leading
alteration
transcriptome
energy
metabolism,
thereby
causing
deficiency
impairing
Small
peptides
(SPs),
ranging
from
5
to
100
amino
acids,
play
integral
roles
in
plants
due
their
diverse
functions.
Despite
low
abundance
and
small
molecular
weight,
SPs
intricately
regulate
critical
aspects
of
plant
life,
including
cell
division,
growth,
differentiation,
flowering,
fruiting,
maturation,
stress
responses.
As
vital
mediators
intercellular
signaling,
have
garnered
significant
attention
biology
research.
This
comprehensive
review
delves
into
SPs'
structure,
classification,
identification,
providing
a
detailed
understanding
significance.
Additionally,
we
summarize
recent
findings
on
the
biological
functions
signaling
pathways
prominent
that
growth
development.
The
also
offers
forward-looking
perspective
future
research
directions
peptide
pathways.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13361 - 13361
Опубликована: Дек. 13, 2024
Subtilases
(SBTs),
known
as
serine
proteases
or
phytoproteases
in
plants,
are
crucial
enzymes
involved
plant
development,
growth,
and
signaling
pathways.
Despite
their
recognized
importance
other
species,
information
regarding
functional
roles
cultivated
peanut
(Arachis
hypogea
L.)
remains
sparse.
We
identified
122
AhSBT
genes
the
STQ
genome,
classifying
them
into
six
subgroups
based
on
phylogenetic
analysis.
Detailed
structural
motif
analyses
revealed
presence
of
conserved
domains,
highlighting
evolutionary
conservation
AhSBTs.
The
collinearity
results
indicate
that
A.
SBT
gene
family
has
17,
5,
1
homologous
pairs
with
Glycine
max,
Arabidopsis
thaliana,
Zea
mays,
respectively.
Furthermore,
prediction
cis-elements
promoters
indicates
they
mainly
associated
hormones
abiotic
stress.
GO
KEGG
showed
many
AhSBTs
important
stress
response.
Based
transcriptome
datasets,
some
genes,
such
AhSBT2,
AhSBT18,
AhSBT19,
AhSBT60,
AhSBT102,
AhSBT5,
AhSBT111,
AhSBT113,
remarkably
higher
expression
diverse
tissues/organs,
i.e.,
embryo,
root,
leaf,
potentially
implicating
seed
development.
Likewise,
only
a
few
including
AhSBT1,
AhSBT39,
AhSBT53,
AhSBT92,
AhSBT115,
were
upregulated
under
(drought
cold)
phytohormone
(ethylene,
abscisic
acid,
paclobutrazol,
brassinolide,
salicylic
acid)
treatments.
Upon
inoculation
Ralstonia
solanacearum,
levels
AhSBT50,
AhSBT115
disease-resistant
downregulated
disease-susceptible
varieties.
qRT-PCR-based
profiling
presented
parallel
trends
generated
from
datasets.
comprehensive
dataset
study
provides
valuable
insights
understanding
AhSBTs,
paving
way
for
potential
applications
crop
improvement.
These
findings
deepen
our
molecular
biology
offer
new
strategies
enhancing
tolerance
agronomically
traits.
