动物学研究,
Год журнала:
2023,
Номер
45(3), С. 586 - 600
Опубликована: Дек. 5, 2023
The
placenta
plays
a
crucial
role
in
successful
mammalian
reproduction.
Ruminant
animals
possess
semi-invasive
characterized
by
highly
vascularized
structure
formed
maternal
endometrial
caruncles
and
fetal
placental
cotyledons,
essential
for
full-term
development.
cow
harbors
at
least
two
trophoblast
cell
populations:
uninucleate
(UNC)
binucleate
(BNC)
cells.
However,
the
limited
capacity
to
elucidate
transcriptomic
dynamics
of
natural
environment
has
resulted
poor
understanding
both
molecular
cellular
interactions
between
cells
niches,
mechanisms
governing
differentiation
functionalization.
To
fill
this
knowledge
gap,
we
employed
Stereo-seq
map
spatial
gene
expression
patterns
near
single-cell
resolution
90
130
days
gestation,
attaining
high-resolution,
spatially
resolved
profiles.
Based
on
clustering
marker
analyses,
key
transcription
factors,
including
YBX1
NPAS2,
were
shown
regulate
heterogeneity
subpopulations.
Cell
communication
trajectory
analysis
provided
framework
cell-cell
trophoblasts
into
BNCs
microenvironment.
Differential
trajectories
identified
set
genes
involved
regulation
differentiation.
Additionally,
modules
co-variant
that
help
shape
specific
tissue
structures
identified.
Together,
these
findings
provide
foundational
insights
important
biological
pathways
critical
development
function
cows.
animal,
Год журнала:
2023,
Номер
17, С. 100796 - 100796
Опубликована: Май 1, 2023
Ruminants
have
a
unique
placenta
in
comparison
to
other
mammalian
species.
Initially,
they
possess
non-invasive
epitheliochorial
type
of
during
conceptus
elongation.
As
the
trophectoderm
begins
attach
luminal
epithelium
(LE)
endometrium,
binucleate
cells
(BNCs)
develop
within
trophoblast
chorion.
The
BNCs
migrate
and
fuse
with
uterine
LE
form
multinucleate
syncytial
plaques
sheep
hybrid
trinucleate
cattle.
This
area
ruminant
is
semi-invasive
synepitheliochorial.
foundation
placental
cotyledons
express
placenta-specific
genes
including
pregnancy-associated
glycoproteins
chorionic
somatomammotropin
hormone
2
or
lactogen.
Attachment
interdigitation
into
endometrial
caruncles
placentomes
that
are
subsequently
vascularized
provide
essential
nutrients
for
growth
fetus.
chapter
review
will
discuss
historical
current
aspects
implantation
development
ungulates
focus
on
cattle
sheep.
Single-cell
analysis
promises
much
more
detailed
understanding
different
cell
populations
insights
pathways
mediating
placenta.
fundamental
required
understand
pregnancy
loss
strategies
improve
outcomes
ruminants.
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 2, 2024
Abstract
Understanding
biological
mechanisms
is
fundamental
for
improving
animal
production
and
health
to
meet
the
growing
demand
high-quality
protein.
As
an
emerging
biotechnology,
single-cell
transcriptomics
has
been
gradually
applied
in
diverse
aspects
of
research,
offering
effective
method
study
gene
expression
high-throughput
single
cells
different
tissues/organs
animals.
In
unprecedented
manner,
researchers
have
identified
cell
types/subtypes
their
marker
genes,
inferred
cellular
fate
trajectories,
revealed
cell‒cell
interactions
animals
using
transcriptomics.
this
paper,
we
introduce
development
technology
review
processes,
advancements,
applications
research.
We
summarize
recent
efforts
obtain
a
more
profound
understanding
nutrition
health,
reproductive
performance,
genetics,
disease
models
livestock
species.
Moreover,
practical
experience
accumulated
based
on
large
number
cases
highlighted
provide
reference
determining
key
factors
(e.g.,
sample
size,
clustering,
type
annotation)
analysis.
also
discuss
limitations
outlook
current
stage.
This
paper
describes
comprehensive
progress
novel
insights
sustainable
advancements
agricultural
productivity
health.
iScience,
Год журнала:
2024,
Номер
27(4), С. 109605 - 109605
Опубликована: Март 27, 2024
Supporting
healthy
pregnancy
outcomes
requires
a
comprehensive
understanding
of
the
molecular
and
cellular
programs
peri-implantation
development,
when
most
failure
occurs.
Here,
we
present
single-cell
transcriptomes
bovine
embryo
development
at
day
12,
14,
16,
18
post-fertilization.
We
defined
composition
gene
expression
embryonic
disc,
hypoblast,
trophoblast
lineages
in
embryos,
identified
markers
pathway
signaling
that
represent
distinct
stages
lineages;
selected
was
validated
embryos.
Using
detailed
time-course
transcriptomic
analyses,
revealed
previously
unrecognized
primitive
cell
lineage.
also
characterized
conserved
divergence
lineage
between
other
mammalian
species.
Finally,
established
cell-cell
communication
underlies
extraembryonic
interaction
to
ensure
proper
early
development.
These
data
provide
foundational
information
discover
essential
biological
underpinning
Reproduction Fertility and Development,
Год журнала:
2023,
Номер
36(2), С. 93 - 111
Опубликована: Окт. 9, 2023
Mammals
differ
regarding
their
placentae,
but
in
all
species
placental
trophoblasts
interact
intimately
with
the
uterine
endometrium
to
mediate
transfer
of
nutrients
from
mother
embryo/fetus
through
closely
juxtaposed
microcirculatory
systems
uterus
and
placenta.
Placentation
ruminants
is
intermediate
between
non-invasive
type,
as
observed
epitheliochorial
placenta
pigs,
invasive
haemochorial
placentae
mice
humans.
In
ruminants,
trophoblast
cells
invade
endometrial
tissue,
invasion
believed
be
limited
luminal
epithelium
(LE).
LE
there
are
varying
degrees
syncytialisation
among
species,
being
more
extensive
sheep
than
cows.
The
hallmarks
placentation
include:
(1)
an
extended
period
which
conceptuses
(embryos
associated
membranes)
elongate
must
supported
by
secretions
(histotroph)
uterus;
(2)
a
cascade
involving
array
adhesion
molecules
that
includes
integrin-mediated
attachment
conceptus
for
implantation;
(3)
developing
early
placenta,
process
currently
understanding;
(4)
development
placentomes
define
cotyledonary
cows
sheep,
provide
haemotrophic
support
fetal
development.
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(49)
Опубликована: Ноя. 29, 2023
The
uterus
is
vital
for
successful
reproduction
in
mammals,
and
two
different
types
of
epithelia
(luminal
glandular)
are
essential
embryo
implantation
pregnancy
establishment.
However,
the
cellular
molecular
factors
pathways
governing
postnatal
epithelium
maturation,
determination,
differentiation
developing
yet
to
be
elucidated.
Here,
neonatal
mouse
was
isolated
subjected
single-cell
transcriptome
(scRNA-seq)
analysis.
Both
undifferentiated
determined
luminal
were
heterogeneous
contained
several
cell
clusters
based
on
transcription
profiles.
Substantial
gene
expression
differences
evident
as
matured
differentiated
between
days
1
15.
Two
new
glandular
epithelium-expressed
genes
(
Gas6
Cited4
)
identified
validated
by
situ
hybridization.
Trajectory
analyses
provided
a
framework
understanding
lineage
bifurcation,
differentiation.
A
candidate
set
regulatory
networks
that
potentially
direct
specification
morphogenesis.
This
atlas
provides
foundation
important
discover
intrinsic
mechanisms
directing
uterine
morphogenesis
during
critical
window
development.
Theriogenology,
Год журнала:
2024,
Номер
223, С. 59 - 69
Опубликована: Апрель 23, 2024
Syncytins
are
endogenous
retroviral
envelope
proteins
which
induce
the
fusion
of
membranes.
A
human
representative
this
group,
retrovirus
group
W
member
1
(ERVW-1)
or
syncytin-1
is
present
in
trophoblast-derived
extracellular
vesicles
and
supports
incorporation
these
into
recipient
cells.
During
pregnancy,
placenta-derived
participate
feto-maternal
communication.
Bovine
fetal
binucleate
trophoblast
cells
express
syncytin,
bovine
protein
K1
(BERV-K1).
These
release
maternal
stroma,
but
it
unclear
whether
BERV-K1
included
vesicles.
Here,
were
isolated
from
placental
tissue
using
collagenase
digestion,
ultracentrifugation,
size
exclusion
chromatography.
They
characterized
with
transmission
electron
microscopy,
nanoparticle
tracking
analysis,
immunoblotting
mass
spectrometry.
Immunohistochemistry
immunoelectron
microscopy
used
to
localize
within
tissue.
The
range
between
50
300
nm,
carrying
multiple
vesicle
biomarkers.
Proteomic
analysis
confirmed
presence
on
Further,
was
localized
intraluminal
secretory
granules
suggests
their
role
communication
potential
involvement
uptake
by
target
