La zonula adherens matura, une nouvelle jonction intercellulaire de l’épithélium intestinal DOI Creative Commons
Pierre Mangeol, Dominique Massey‐Harroche, Michaël Sebbagh

и другие.

médecine/sciences, Год журнала: 2024, Номер 40(12), С. 985 - 988

Опубликована: Дек. 1, 2024

été beaucoup étudiée, notamment en raison de son rôle au cours du développement.Son organisation moléculaire a formulée dans

A short guide to the tight junction DOI Creative Commons
Sandra Citi, Michael Fromm, Mikio Furuse

и другие.

Journal of Cell Science, Год журнала: 2024, Номер 137(9)

Опубликована: Май 1, 2024

ABSTRACT Tight junctions (TJs) are specialized regions of contact between cells epithelial and endothelial tissues that form selective semipermeable paracellular barriers establish maintain body compartments with different fluid compositions. As such, the formation TJs represents a critical step in metazoan evolution, allowing multicompartmental organisms true, barrier-forming epithelia endothelia. In six decades have passed since first observations by transmission electron microscopy, much progress has been made understanding structure, function, molecular composition regulation TJs. The goal this Perspective is to highlight key concepts emerged through research future challenges lie ahead for field.

Язык: Английский

Процитировано

32

α-catenin phosphorylation is elevated during mitosis to resist apical rounding and epithelial barrier leak DOI Creative Commons

Phuong M. Le,

Jeanne M. Quinn,

Annette S. Flozak

и другие.

Biology Open, Год журнала: 2025, Номер 14(1)

Опубликована: Янв. 8, 2025

ABSTRACT Epithelial cell cohesion and barrier function critically depend on α-catenin, an actin-binding protein essential constituent of cadherin-catenin-based adherens junctions. α-catenin undergoes actomyosin force-dependent unfolding both middle domains to strongly engage actin filaments its various effectors; this mechanosensitivity is critical for junction function. We previously showed that highly phosphorylated in unstructured region links the mechanosensitive (known as P-linker region), but cellular processes promote phosphorylation have remained elusive. Here, we leverage a published phospho-proteomic data set show maximally during mitosis. By reconstituting CRISPR knockout MDCK cells with wild-type, phospho-mutant phospho-mimic forms full restrains mitotic rounding apical direction, strengthening interactions between dividing non-dividing neighbors limit epithelial leak. As major scaffold components junctions, tight junctions desmosomes are also differentially mitosis, reason division may be tractable system understand how complexes coordinately regulated sustain under tension-generating morphogenetic processes.

Язык: Английский

Процитировано

1

Proximity proteomics provides a new resource for exploring the function of Afadin and the complexity of cell-cell adherens junctions DOI Creative Commons
Wangsun Choi, Dennis Goldfarb,

Feng Yan

и другие.

Biology Open, Год журнала: 2025, Номер 14(2)

Опубликована: Янв. 30, 2025

The network of proteins at the interface between cell-cell adherens junctions and actomyosin cytoskeleton provides robust yet dynamic connections that facilitate cell shape change motility. While this was initially thought to be a simple linear connection via classic cadherins their associated catenins, we now have come appreciate many more are involved, providing robustness mechanosensitivity. Defining full set in remains key objective our field. Proximity proteomics means define these networks. Mammalian Afadin its Drosophila homolog Canoe parts protein network, facilitating diverse changes during gastrulation other events embryonic morphogenesis. Here report results several proximity screens, defining neighborhood both N- C-termini mammalian premier epithelial model, MDCK cells. We compare with previous screens done types, efforts junctional proteins. These reveal value multiple neighbors offer interesting insights into overlap composition different junctions.

Язык: Английский

Процитировано

1

Co-Exposure to Polystyrene Microplastics and Bisphenol A Contributes to the Formation of Liver Fibrosis in Mice through Inhibition of the BMAL1/E-Cad Signaling Pathway DOI

Bonan Xiao,

Wanghao Yang,

Hao Dong

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 12, 2025

The food safety risks posed by exposure to polystyrene microplastics (PS-MPs) and bisphenol A (BPA) have become an issue worldwide. However, the toxic effects of PS-MPs BPA coexposure on mammalian liver remain elusive. In this study, we found that synergistic AML12 cells mouse liver. Histopathological staining revealed excessive accumulation extracellular matrix in Co-exposure downregulated Bmal1 E-cad both vitro vivo. Additionally, Bmal1–/– liver-specific mice exhibited significantly reduced levels, with no significant reduction under coexposure. Notably, overexpression BMAL1 CLOCK enhanced luciferase activity driven gene intron region (containing E-box cis-element). These results demonstrated contributed development fibrosis inhibiting BMAL1/E-cad signaling pathway.

Язык: Английский

Процитировано

1

Damage control of epithelial barrier function in dynamic environments DOI Creative Commons
Tomohito Higashi, Akira Saitō, Hideki Chiba

и другие.

European Journal of Cell Biology, Год журнала: 2024, Номер 103(2), С. 151410 - 151410

Опубликована: Апрель 3, 2024

Epithelial tissues cover the surfaces and lumens of internal organs multicellular animals crucially contribute to environment homeostasis by delineating distinct compartments within body. This vital role is known as epithelial barrier function. cells are arranged like cobblestones intricately bind together form an sheet that upholds this Central restriction solute fluid diffusion through intercellular spaces occluding junctions, tight junctions in vertebrates septate invertebrates. As part tissues, undergo constant renewal, with older being replaced new ones. Simultaneously, tissue undergoes relative rearrangement, elongating, shifting directionally a whole. The movement or shape changes necessitate significant deformation reconnection junctions. Recent advancements have shed light on intricate mechanisms which sustain their function dynamic environments. review aims introduce these noteworthy findings discuss some questions remain unanswered.

Язык: Английский

Процитировано

6

Afadin–nectin forces its way to the front DOI Open Access
Michaël Sebbagh, Martin A. Schwartz

The Journal of Cell Biology, Год журнала: 2024, Номер 223(5)

Опубликована: Апрель 2, 2024

Force transmission at cell–cell junctions critically regulates embryogenesis, tissue homeostasis, and diseases including cancer. The cadherin–catenin linkage has been considered the keystone of junctional force transmission, but new findings challenge this paradigm, arguing instead that nectin–afadin plays more important role in mature intestinal epithelium.

Язык: Английский

Процитировано

3

Multivalent Afadin Interaction Promotes IDR-Mediated Condensate Formation and Junctional Separation of Epithelial Cells DOI
Shuhei Kuno,

Ryu Nakamura,

Tetsuhisa Otani

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 28, 2024

Summary In epithelial cells, cell-cell adhesion is mediated by the apical junctional complex (AJC), which consists of tight junctions (TJs) and adherens (AJs) aligned from to basal axis. However, mechanism AJC formation on side separation these within AJCs are poorly understood. We found that multivalent interactions afadin with molecules cytoskeleton lead condensate in an intrinsically disordered region (IDR)-dependent manner, promotes efficient accumulation linear AJ during initial junction formation. Furthermore, we ZO-1 were able induce different formations cell differentially distributed each other. These properties explain how it strictly localizes AJs cells involved regulating segregation TJ AJC.

Язык: Английский

Процитировано

3

Vertex remodeling during epithelial morphogenesis DOI Creative Commons
Kaoru Sugimura, Tetsuhisa Otani

Current Opinion in Cell Biology, Год журнала: 2024, Номер 91, С. 102427 - 102427

Опубликована: Сен. 26, 2024

Epithelial cells adhere to each other via intercellular junctions that can be classified into bicellular and tricellular contacts (vertices). morphogenesis involves cell rearrangement requires remodeling of vertices. Although our understanding how junction mechanics drive epithelial has advanced, the mechanisms underlying vertex during this process have only received attention recently. In review, we outline recent progress in reorganize adhesion cytoskeleton trigger displacement resolution We will also discuss achieve optimal balance between structural flexibility stability their Finally, introduce new modeling frameworks designed analyze at Integration live imaging techniques is providing insights active roles vertices morphogenesis.

Язык: Английский

Процитировано

3

Afadin-deficient retinas exhibit severe neuronal lamination defects but preserve visual functions DOI Open Access

Akiko Ueno,

Konan Sakuta,

Ono H

и другие.

Опубликована: Фев. 14, 2025

Neural lamination is a common feature of the central nervous system (CNS), with several subcellular structures, such as adherens junctions (AJs), playing role in this process. The retina also heavily laminated, but it remains unclear how laminar formation impacts retinal cell morphology, synapse integrity, and overall function. In study, we demonstrate that loss afadin, key component AJs, leads to significant pathological changes. These include disruption outer notable decrease well mislocalization photoreceptors, their segments, photoreceptor synapses. Interestingly, despite these severe impairments, recorded small local field potentials, including a- b-waves. We classified ganglion cells into ON, ON-OFF, OFF types based on firing patterns response light stimuli. Additionally, successfully characterized receptive fields certain cells. Overall, findings provide first evidence circuit function can be partially preserved even when there are disruptions Our results indicate retinas severely altered morphology still retain some capacity process

Язык: Английский

Процитировано

0

Afadin-deficient retinas exhibit severe neuronal lamination defects but preserve visual functions DOI Open Access

Akiko Ueno,

Konan Sakuta,

Ono H

и другие.

Опубликована: Фев. 14, 2025

Neural lamination is a common feature of the central nervous system (CNS), with several subcellular structures, such as adherens junctions (AJs), playing role in this process. The retina also heavily laminated, but it remains unclear how laminar formation impacts retinal cell morphology, synapse integrity, and overall function. In study, we demonstrate that loss afadin, key component AJs, leads to significant pathological changes. These include disruption outer notable decrease well mislocalization photoreceptors, their segments, photoreceptor synapses. Interestingly, despite these severe impairments, recorded small local field potentials, including a- b-waves. We classified ganglion cells into ON, ON-OFF, OFF types based on firing patterns response light stimuli. Additionally, successfully characterized receptive fields certain cells. Overall, findings provide first evidence circuit function can be partially preserved even when there are disruptions Our results indicate retinas severely altered morphology still retain some capacity process

Язык: Английский

Процитировано

0