Metals in Motion: Understanding Labile Metal Pools in Bacteria
Biochemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 5, 2025
Metal
ions
are
essential
for
all
life.
In
microbial
cells,
potassium
(K+)
is
the
most
abundant
cation
and
plays
a
key
role
in
maintaining
osmotic
balance.
Magnesium
(Mg2+)
dominant
divalent
required
nucleic
acid
structure
as
an
enzyme
cofactor.
Microbes
typically
require
transition
metals
manganese
(Mn),
iron
(Fe),
copper
(Cu),
zinc
(Zn),
although
precise
set
of
metal
needed
to
sustain
life
variable.
Intracellular
pools
can
be
conceptualized
chemically
complex
mixture
rapidly
exchanging
(labile)
ions,
complemented
by
those
reservoirs
that
exchange
slowly
relative
cell
metabolism
(sequestered).
Labile
buffered
transient
interactions
with
anionic
metabolites
macromolecules,
ribosome
playing
major
role.
Sequestered
include
many
metalloproteins,
cofactors,
storage
depots,
some
redeployed
upon
depletion.
Here,
I
review
size,
composition,
dynamics
intracellular
highlight
gaps
understanding.
Язык: Английский
Realgar Transforming Solution Triggered Ferroptosis in Leukemia Cells by Disrupting Iron Homeostasis Through the Slc7a11/Gpx4 Axis
Опубликована: Янв. 1, 2025
Clinical
investigations
have
demonstrated
that
the
progression
of
acute
myeloid
leukemia
(AML)
is
particularly
susceptible
to
iron
overload
and
dysregulation
homeostasis,
which
can
trigger
ferroptosis.
Consequently,
regulating
homeostasis
may
positively
impact
AML
treatment.
This
study
aimed
investigate
anticancer
properties
potential
mechanisms
realgar
transforming
solution
(RTS)
in
inducing
ferroptosis
disrupting
NB4
cells.
Cell
viability
was
assessed
using
Counting
Kit-8
(CCK-8)
assay.
Transcriptomic
analysis
conducted
identify
differentially
expressed
genes
(DEGs)
predict
pathways
through
RTS
exerts
its
antitumor
effects.
Ferroptosis
evaluated
BODIPY
C11
lipid
ROS
probe,
T-SOD,
MDA,
GSH
assay
kits,
along
with
JC-1
fluorescent
probe
Western
blot
analysis.
Iron
Fe2+
kits
metabolism.
Transcriptome
sequencing
revealed
819
DEGs,
HO-1
PML-RARα
Regulated
Adaptor
Molecule
1
(PRAM1)
being
most
prominent.
KEGG
indicated
significant
enrichment
pathway.
In
vitro
studies
treatment
resulted
increased
levels
depletion
SLC7A11
GPX4
expression
Additionally,
TFR1
NCOA4
increased,
while
FPN1
(SLC40A1),
FTH1,
IREB2
decreased
under
led
intracellular
accumulation,
ultimately
Pretreatment
inhibitors,
such
as
Ferrostatin-1
(Fer-1)
or
Deferoxamine
mesylate
(DFOM),
attenuated
RTS-induced
The
demonstrates
induce
cells
by
via
regulation
SLC7A11/GPX4
axis.
These
findings
suggest
a
therapeutic
agent
for
AML.
Язык: Английский
Excellent Fe(II) Binding Tag in Protein Paramagnetic NMR Spectroscopy
Inorganic Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 14, 2025
Most
first
series
of
transition
metal
ions
have
one
or
more
unpaired
electrons
and
show
great
variations
in
the
paramagnetic
property.
The
magnetic
anisotropy
some
ions,
including
Co(II),
as
well
lanthanide
[Ln(III)],
has
been
examined
proteins
by
NMR.
In
contrast,
few
examples
Fe(II)
complexes
reporting
were
analyzed
proteins,
except
for
ones
containing
a
heme
motif
iron-sulfur
clusters.
Here,
we
showed
that
[2,2':6',2″-terpyridine]-6,6″-dicarboxylic
acid
(TDA)
is
an
excellent
iron-binding
ligand.
It
forms
stable
iron
complex
aqueous
solution
demonstrates
distinct
properties.
TDA
1:1
with
both
Fe(III),
but
presents
high-spin
state
complex.
moiety
can
be
site-specifically
attached
to
protein,
its
protein
conjugate
generates
sizable
pseudocontact
shifts
(PCSs)
Fe(II),
which
are
larger
than
those
commonly
used
binding
tags.
protein-TDA-Fe(III)
produces
negligible
relaxation
enhancement
(PRE)
effects
signals,
indicating
low-spin
Fe(III)
protein-TDA
high
stability
protein-TDA-Fe(II)
allows
measure
accurate
PCSs
cell
lysate
even
presence
other
excess
GSH.
Язык: Английский