Morphological and metabolic changes in Changshan Huyou (Citrus aurantium) following natural tetraploidization DOI

Peiru Huang,

Tianyu Xu, Gang Wang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 3, 2024

Abstract Background Polyploids in citrus are generally used to improve crop varieties. Changshan Huyou (Citrus aurantium) is a native species China that highly adaptable and has pharmaceutical value. However, the influence following polyploidization remains unclear. Here we evaluated phenotypic variations metabolic alterations autotetraploidization of seedlings fruits analyzed associated transcriptomic changes. Result The tetraploid had rounder thicker leaves, larger floral organs fruits, satisfactory viability pollen grains ovules. accumulated lower levels soluble solids but similar organic acids. Metabolic profiling three tissues revealed most 2064 differentially metabolites (DAMs), including flavonoids, lignans, coumarins, were downregulated. In contrast, upregulated DAMs mainly included alkaloids (clausine K 2-(1-pentenyl)quinoline), amino acids (L-asparagine L-ornithine), terpenoids (deacetylnomilin evodol) peels, as well as, flavonoids (neohesperidin quercetin-5-O-β-D-glucoside) (2-methylsuccinic acid dimethylmalonic acid) juice sacs. genes with phenylpropanoid biosynthesis, secondary metabolite biosynthesis various alkaloid pathways. results Pearson correlation analysis showed encoded peroxidase cytochrome P450 closely related higher accumulation neohesperidin quercetin glucoside increased by ferulate-5-hydroxylase, CYP450 81Q32, flavonoid 3'-monooxygenase, 4-coumarate–CoA ligase 1, UDP-glucose flavonoid 3-O-glucosyltransferase, some transcription factors Conclusion investigated here may be breeding triploid citrus, especially produce seedless varieties, for purposes fruit processing, they polyploidization.

Язык: Английский

A long road ahead to reliable and complete medicinal plant genomes DOI Creative Commons

L. Cheng,

Zilong Wang, Qian‐Hao Zhu

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 4, 2025

Язык: Английский

Процитировано

1
Polymethoxylated flavone variations and in vitro biological activities of locally cultivated Citrus varieties in China DOI
Dan Wang, Zhenqing Li,

Zixiao Jiang

и другие.

Food Chemistry, Год журнала: 2024, Номер 463, С. 141047 - 141047

Опубликована: Авг. 31, 2024

Язык: Английский

Процитировано

6
Artificial intelligence in the discovery and modification of biological elements in medicinal plants DOI Creative Commons
Jing Zhang, Yixin Yang, Jinping Si

и другие.

Medicinal Plant Biology, Год журнала: 2025, Номер 4(1), С. 0 - 0

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Transcriptomic and metabolomic analyses reveal the mechanism of color difference between two kinds of Cistanche deserticola before and after drying DOI Creative Commons
Tiantian Zhu, Jing Zhang, Tianle Liu

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 23, 2025

Introduction Cistanche deserticola is an important traditional Chinese herbal medicine. The fresh cistanche squamous stem typically yellow-white and brown after drying. Oil a variant with purple that turns black color difference between oil obvious, the former has higher market price. However, mechanism underlying of remains unclear. Methods This study evaluated total flavone contents in compared differential metabolites differentially expressed genes (DEGs) iridoid dried samples were determined by high-performance liquid chromatography, finally polysaccharides them to comprehensively analyze formation cistanche. Results results showed flavonoid content was significantly than Metabolomic analysis identified 50 accumulated (DAMs) (34 up-regulated 16 down-regulated), including carbohydrates, terpenoids, flavonoids. Moreover, 3,376 DEGs selected, among which significant IGS1 CYP84A1 down-regulated 4CLL1 , F6H2-2-1 5MAT1 jointly regulated biosynthesis affected accumulation metabolites. Significant CCD7 gene carotenoid component production, UGT85A24 promoted geniposidic acid. In addition, polysaccharide those Discussion expression flavonoids terpenoid FLS mainly caused Dried darker cistanche, due iridoids former. preliminarily revealed causes differences provided references for systematic its germplasm resources, as well breeding C. .

Язык: Английский

Процитировано

0
Citrus genomes: Past, present and future DOI Creative Commons
Upuli Nakandala, Agnelo Furtado, Robert J Henry

и другие.

Horticulture Research, Год журнала: 2025, Номер 12(5)

Опубликована: Фев. 4, 2025

Abstract Over the past decade, genome sequencing and assembly approaches have been greatly improved, resulting in of many genomes for citrus, including wild, domesticated, citrus-related genomes. Improvements technologies led to assembled with higher completeness, contiguity, quality, accuracy that facilitated annotation analysis. This review summarizes evolution sequencing, assembly, leading citrus over 11 years, a comprehensive evaluation their major findings applications. Of 50 now available, 35 chromosome level 15 draft level, 14 were haplotype-resolved assemblies. To date there four pangenome-wide studies citrus. The very recent long-read achieved >99% >98% completeness (BUSCO), respectively. However, some early are not same high quality as more recently sequenced would benefit from re-sequencing. A pangenome based upon larger set species genotypes at haplotype allow genomics deliver maximum benefits improvement research.

Язык: Английский

Процитировано

0
Morphological and metabolic changes in Changshan Huyou (Citrus changshan-huyou) following natural tetraploidization DOI Creative Commons

Peiru Huang,

Tianyu Xu, Gang Wang

и другие.

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 8, 2025

Polyploids in citrus are generally used to improve crop varieties. Changshan Huyou (Citrus changshan-huyou) is a native species China that highly adaptable and has pharmaceutical value. However, the influence following polyploidization remains unclear. Here we evaluated adult tetraploid scions of with contemporary diploid as control phenotypic variations, metabolic alterations fruits associated transcriptomic changes. The had rounder thicker leaves, larger floral organs fruits, satisfactory viability pollen grains ovules. accumulated lower levels soluble solids but similar organic acids. Metabolic profiling three tissues revealed most 2064 differentially metabolites (DAMs), including flavonoids, lignans, coumarins, were downregulated. In contrast, upregulated DAMs mainly included alkaloids (clausine K 2-(1-pentenyl)quinoline), amino acids (L-asparagine L-ornithine), terpenoids (deacetylnomilin evodol) peels, well as, flavonoids (neohesperidin quercetin-5-O-β-D-glucoside) (2-methylsuccinic acid dimethylmalonic acid) juice sacs. genes phenylpropanoid biosynthesis, secondary metabolite biosynthesis various alkaloid pathways. Pearson Correlation Analysis showed encoding PEROXIDASE CYTOCHROME P450 (CYP450) closely related higher accumulation up-regulated neohesperidin quercetin glucoside positively FERULATE-5-HYDROXYLASE (F5H), CYP450 81Q32, FLAVONOID 3'-MONOOXYGENASE (F3'H), 4-COUMARATE-CoA LIGASE 1 (4CL1), UDP-GLUCOSE 3-O-GLUCOSYLTRANSFERASE (UFOG), some transcription factors investigated here may be triploids breeding produce seedless citrus, for fruit processing on purpose due alteration polyploidization.

Язык: Английский

Процитировано

0
Two Caffeoyl-CoA O-methyltransferase-like enzyme are involved in the biosynthesis of polymethoxyflavones in Citrus reticulata ‘Chachiensis’ DOI
Huimin Pan, Jianmu Su,

Mei Bai

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 310, С. 143277 - 143277

Опубликована: Апрель 25, 2025

Язык: Английский

Процитировано

0
Molecular and Structural Characterization of a Chalcone di-C-Methyltransferase RdCMT from Rhododendron dauricum and Its Application in De Novo Biosynthesis of Farrerol in Pichia pastoris DOI
Meng Zhang, Yang‐Oujie Bao, Zonglin Dai

и другие.

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Methylation plays a crucial role in drug design and optimization. While numerous methyltransferases have been characterized from plants, C-methyltransferases, particularly those targeting phenolic skeletons, are rare. In this study, we identified novel di-C-methyltransferase RdCMT the medicinal plant Rhododendron dauricum. catalyzes sequential two-step 3'-C/5'-C-methylation of naringenin chalcone, leading to biosynthesis farrerol. exhibited strict substrate specificity for chalcones. Through combinatorial catalysis, series C-methylated flavonoids were synthesized. Moreover, farrerol was synthesized de novo Nicotiana benthamiana Pichia pastoris with yields 0.4 mg/g (dry weight) 149.0 mg/L, respectively. The structure determined using cryo-electron microscopy (cryo-EM), revealing that residues R328 G296 significantly influence RdCMT. This work not only introduces potent biocatalyst preparation but also offers insights into catalytic mechanisms C-methyltransferases.

Язык: Английский

Процитировано

0
Functional study of an O-methyltransferase in eupatilin biosynthesis in Artemisia argyi by an efficient hairy roots transformation system DOI

Sainan Peng,

Jingyi Zhang,

Yun Yang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 315, С. 144437 - 144437

Опубликована: Май 24, 2025

Язык: Английский

Процитировано

0
Microplastics as Emerging Contaminants and Human Health: Exploring Functional Nutrition in Gastric–Colon–Brain Axis Cancer DOI Creative Commons
Maria Scuto,

Claudio Lombardo,

Bruna Lo Sasso

и другие.

Toxics, Год журнала: 2025, Номер 13(6), С. 438 - 438

Опубликована: Май 26, 2025

Microplastics (MPs), emerging contaminants of significant global concern, have a substantially increased environmental impact due to their biological persistence and accumulation in the body. Exposure MPs has been associated with oxidative stress, systemic inflammation, cellular dysfunction, notably affecting critical tissues such as stomach, colon, brain. This review explores correlation between cancer risk along gastric–colon–brain axis, identifying signaling pathways altered by MP exposure. Furthermore, it highlights role functional nutrition bioactive flavonoids—including chlorogenic acid, coumaric naringin—as well use highly bioavailable combined polyphenol nanoparticles potential detoxifying agents. Functional nutrients are effective enhancing resilience against reactive oxygen species (ROS) production MP-induced toxicity, offering protective effects at gastric, intestinal, brain barriers. Activation Nrf2 pathway compounds promotes expression enzymes, suggesting promising nutritional strategy mitigate MP-related damage. underscores how may represent viable therapeutic approach reduce harmful The integration advanced technologies—such microfluidic systems, organ-on-a-chip platforms, machine learning—and identification key molecular targets lay foundation for developing preventive personalized medicine strategies aimed lowering environmentally induced carcinogenesis.

Язык: Английский

Процитировано

0