Morphological and metabolic changes in Changshan Huyou (Citrus aurantium) following natural tetraploidization DOI

Peiru Huang,

Tianyu Xu, Gang Wang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 3, 2024

Abstract Background Polyploids in citrus are generally used to improve crop varieties. Changshan Huyou (Citrus aurantium) is a native species China that highly adaptable and has pharmaceutical value. However, the influence following polyploidization remains unclear. Here we evaluated phenotypic variations metabolic alterations autotetraploidization of seedlings fruits analyzed associated transcriptomic changes. Result The tetraploid had rounder thicker leaves, larger floral organs fruits, satisfactory viability pollen grains ovules. accumulated lower levels soluble solids but similar organic acids. Metabolic profiling three tissues revealed most 2064 differentially metabolites (DAMs), including flavonoids, lignans, coumarins, were downregulated. In contrast, upregulated DAMs mainly included alkaloids (clausine K 2-(1-pentenyl)quinoline), amino acids (L-asparagine L-ornithine), terpenoids (deacetylnomilin evodol) peels, as well as, flavonoids (neohesperidin quercetin-5-O-β-D-glucoside) (2-methylsuccinic acid dimethylmalonic acid) juice sacs. genes with phenylpropanoid biosynthesis, secondary metabolite biosynthesis various alkaloid pathways. results Pearson correlation analysis showed encoded peroxidase cytochrome P450 closely related higher accumulation neohesperidin quercetin glucoside increased by ferulate-5-hydroxylase, CYP450 81Q32, flavonoid 3'-monooxygenase, 4-coumarate–CoA ligase 1, UDP-glucose flavonoid 3-O-glucosyltransferase, some transcription factors Conclusion investigated here may be breeding triploid citrus, especially produce seedless varieties, for purposes fruit processing, they polyploidization.

Language: Английский

A long road ahead to reliable and complete medicinal plant genomes DOI Creative Commons

L. Cheng,

Zilong Wang, Qian‐Hao Zhu

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 4, 2025

Language: Английский

Citations

1
Polymethoxylated flavone variations and in vitro biological activities of locally cultivated Citrus varieties in China DOI
Dan Wang, Zhenqing Li,

Zixiao Jiang

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 463, P. 141047 - 141047

Published: Aug. 31, 2024

Language: Английский

Citations

4
Transcriptomic and metabolomic analyses reveal the mechanism of color difference between two kinds of Cistanche deserticola before and after drying DOI Creative Commons
Tiantian Zhu, Jing Zhang, Tianle Liu

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 23, 2025

Introduction Cistanche deserticola is an important traditional Chinese herbal medicine. The fresh cistanche squamous stem typically yellow-white and brown after drying. Oil a variant with purple that turns black color difference between oil obvious, the former has higher market price. However, mechanism underlying of remains unclear. Methods This study evaluated total flavone contents in compared differential metabolites differentially expressed genes (DEGs) iridoid dried samples were determined by high-performance liquid chromatography, finally polysaccharides them to comprehensively analyze formation cistanche. Results results showed flavonoid content was significantly than Metabolomic analysis identified 50 accumulated (DAMs) (34 up-regulated 16 down-regulated), including carbohydrates, terpenoids, flavonoids. Moreover, 3,376 DEGs selected, among which significant IGS1 CYP84A1 down-regulated 4CLL1 , F6H2-2-1 5MAT1 jointly regulated biosynthesis affected accumulation metabolites. Significant CCD7 gene carotenoid component production, UGT85A24 promoted geniposidic acid. In addition, polysaccharide those Discussion expression flavonoids terpenoid FLS mainly caused Dried darker cistanche, due iridoids former. preliminarily revealed causes differences provided references for systematic its germplasm resources, as well breeding C. .

Language: Английский

Citations

0
Citrus genomes: Past, present and future DOI Creative Commons
Upuli Nakandala, Agnelo Furtado, Robert J Henry

et al.

Horticulture Research, Journal Year: 2025, Volume and Issue: 12(5)

Published: Feb. 4, 2025

Abstract Over the past decade, genome sequencing and assembly approaches have been greatly improved, resulting in of many genomes for citrus, including wild, domesticated, citrus-related genomes. Improvements technologies led to assembled with higher completeness, contiguity, quality, accuracy that facilitated annotation analysis. This review summarizes evolution sequencing, assembly, leading citrus over 11 years, a comprehensive evaluation their major findings applications. Of 50 now available, 35 chromosome level 15 draft level, 14 were haplotype-resolved assemblies. To date there four pangenome-wide studies citrus. The very recent long-read achieved >99% >98% completeness (BUSCO), respectively. However, some early are not same high quality as more recently sequenced would benefit from re-sequencing. A pangenome based upon larger set species genotypes at haplotype allow genomics deliver maximum benefits improvement research.

Language: Английский

Citations

0
Morphological and metabolic changes in Changshan Huyou (Citrus changshan-huyou) following natural tetraploidization DOI Creative Commons

Peiru Huang,

Tianyu Xu, Gang Wang

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 8, 2025

Polyploids in citrus are generally used to improve crop varieties. Changshan Huyou (Citrus changshan-huyou) is a native species China that highly adaptable and has pharmaceutical value. However, the influence following polyploidization remains unclear. Here we evaluated adult tetraploid scions of with contemporary diploid as control phenotypic variations, metabolic alterations fruits associated transcriptomic changes. The had rounder thicker leaves, larger floral organs fruits, satisfactory viability pollen grains ovules. accumulated lower levels soluble solids but similar organic acids. Metabolic profiling three tissues revealed most 2064 differentially metabolites (DAMs), including flavonoids, lignans, coumarins, were downregulated. In contrast, upregulated DAMs mainly included alkaloids (clausine K 2-(1-pentenyl)quinoline), amino acids (L-asparagine L-ornithine), terpenoids (deacetylnomilin evodol) peels, well as, flavonoids (neohesperidin quercetin-5-O-β-D-glucoside) (2-methylsuccinic acid dimethylmalonic acid) juice sacs. genes phenylpropanoid biosynthesis, secondary metabolite biosynthesis various alkaloid pathways. Pearson Correlation Analysis showed encoding PEROXIDASE CYTOCHROME P450 (CYP450) closely related higher accumulation up-regulated neohesperidin quercetin glucoside positively FERULATE-5-HYDROXYLASE (F5H), CYP450 81Q32, FLAVONOID 3'-MONOOXYGENASE (F3'H), 4-COUMARATE-CoA LIGASE 1 (4CL1), UDP-GLUCOSE 3-O-GLUCOSYLTRANSFERASE (UFOG), some transcription factors investigated here may be triploids breeding produce seedless citrus, for fruit processing on purpose due alteration polyploidization.

Language: Английский

Citations

0
Artificial intelligence in the discovery and modification of biological elements in medicinal plants DOI Creative Commons
Jing Zhang, Yixin Yang, Jinping Si

et al.

Medicinal Plant Biology, Journal Year: 2025, Volume and Issue: 4(1), P. 0 - 0

Published: Jan. 1, 2025

Language: Английский

Citations

0
Two Caffeoyl-CoA O-methyltransferase-like enzyme are involved in the biosynthesis of polymethoxyflavones in Citrus reticulata ‘Chachiensis’ DOI
Huimin Pan, Jianmu Su,

Mei Bai

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143277 - 143277

Published: April 25, 2025

Language: Английский

Citations

0
Morphological and metabolic changes in Changshan Huyou (Citrus aurantium) following natural tetraploidization DOI

Peiru Huang,

Tianyu Xu, Gang Wang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 3, 2024

Abstract Background Polyploids in citrus are generally used to improve crop varieties. Changshan Huyou (Citrus aurantium) is a native species China that highly adaptable and has pharmaceutical value. However, the influence following polyploidization remains unclear. Here we evaluated phenotypic variations metabolic alterations autotetraploidization of seedlings fruits analyzed associated transcriptomic changes. Result The tetraploid had rounder thicker leaves, larger floral organs fruits, satisfactory viability pollen grains ovules. accumulated lower levels soluble solids but similar organic acids. Metabolic profiling three tissues revealed most 2064 differentially metabolites (DAMs), including flavonoids, lignans, coumarins, were downregulated. In contrast, upregulated DAMs mainly included alkaloids (clausine K 2-(1-pentenyl)quinoline), amino acids (L-asparagine L-ornithine), terpenoids (deacetylnomilin evodol) peels, as well as, flavonoids (neohesperidin quercetin-5-O-β-D-glucoside) (2-methylsuccinic acid dimethylmalonic acid) juice sacs. genes with phenylpropanoid biosynthesis, secondary metabolite biosynthesis various alkaloid pathways. results Pearson correlation analysis showed encoded peroxidase cytochrome P450 closely related higher accumulation neohesperidin quercetin glucoside increased by ferulate-5-hydroxylase, CYP450 81Q32, flavonoid 3'-monooxygenase, 4-coumarate–CoA ligase 1, UDP-glucose flavonoid 3-O-glucosyltransferase, some transcription factors Conclusion investigated here may be breeding triploid citrus, especially produce seedless varieties, for purposes fruit processing, they polyploidization.

Language: Английский

Citations

0