A new class of type VI secretion system effectors can carry two toxic domains and are recognized through the WHIX motif for export DOI Creative Commons

Chaya Mushka Fridman,

Kinga Keppel,

Vladislav Rudenko

и другие.

PLoS Biology, Год журнала: 2025, Номер 23(3), С. e3003053 - e3003053

Опубликована: Март 17, 2025

Gram-negative bacteria employ the type VI secretion system (T6SS) to deliver toxic effectors into neighboring cells and outcompete rivals. Although many have been identified, their mechanism often remains unknown. Here, we describe WHIX, a domain sufficient mediate of via T6SS. Remarkably, find WHIX in T6SS that contain single domain, as well two distinct domains fused either side WHIX. We demonstrate latter, which name double-blade effectors, require cognate immunity proteins antagonize toxicity. Furthermore, show can be used chassis for T6SS-mediated multiple domains. Our findings reveal new class polymorphic cargo with unique deploy one shot, possibly reducing recipients’ ability defend themselves.

Язык: Английский

Decoding resistance in the age of T6SS warfare DOI Creative Commons
Nicholas J. Shikuma

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(7)

Опубликована: Фев. 10, 2025

Язык: Английский

Процитировано

0
A new class of type VI secretion system effectors can carry two toxic domains and are recognized through the WHIX motif for export DOI Creative Commons

Chaya Mushka Fridman,

Kinga Keppel,

Vladislav Rudenko

и другие.

PLoS Biology, Год журнала: 2025, Номер 23(3), С. e3003053 - e3003053

Опубликована: Март 17, 2025

Gram-negative bacteria employ the type VI secretion system (T6SS) to deliver toxic effectors into neighboring cells and outcompete rivals. Although many have been identified, their mechanism often remains unknown. Here, we describe WHIX, a domain sufficient mediate of via T6SS. Remarkably, find WHIX in T6SS that contain single domain, as well two distinct domains fused either side WHIX. We demonstrate latter, which name double-blade effectors, require cognate immunity proteins antagonize toxicity. Furthermore, show can be used chassis for T6SS-mediated multiple domains. Our findings reveal new class polymorphic cargo with unique deploy one shot, possibly reducing recipients’ ability defend themselves.

Язык: Английский

Процитировано

0