A new class of type VI secretion system effectors can carry two toxic domains and are recognized through the WHIX motif for export DOI Creative Commons

Chaya Mushka Fridman,

Kinga Keppel,

Vladislav Rudenko

et al.

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(3), P. e3003053 - e3003053

Published: March 17, 2025

Gram-negative bacteria employ the type VI secretion system (T6SS) to deliver toxic effectors into neighboring cells and outcompete rivals. Although many have been identified, their mechanism often remains unknown. Here, we describe WHIX, a domain sufficient mediate of via T6SS. Remarkably, find WHIX in T6SS that contain single domain, as well two distinct domains fused either side WHIX. We demonstrate latter, which name double-blade effectors, require cognate immunity proteins antagonize toxicity. Furthermore, show can be used chassis for T6SS-mediated multiple domains. Our findings reveal new class polymorphic cargo with unique deploy one shot, possibly reducing recipients’ ability defend themselves.

Language: Английский

Decoding resistance in the age of T6SS warfare DOI Creative Commons
Nicholas J. Shikuma

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(7)

Published: Feb. 10, 2025

Language: Английский

Citations

0
A new class of type VI secretion system effectors can carry two toxic domains and are recognized through the WHIX motif for export DOI Creative Commons

Chaya Mushka Fridman,

Kinga Keppel,

Vladislav Rudenko

et al.

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(3), P. e3003053 - e3003053

Published: March 17, 2025

Gram-negative bacteria employ the type VI secretion system (T6SS) to deliver toxic effectors into neighboring cells and outcompete rivals. Although many have been identified, their mechanism often remains unknown. Here, we describe WHIX, a domain sufficient mediate of via T6SS. Remarkably, find WHIX in T6SS that contain single domain, as well two distinct domains fused either side WHIX. We demonstrate latter, which name double-blade effectors, require cognate immunity proteins antagonize toxicity. Furthermore, show can be used chassis for T6SS-mediated multiple domains. Our findings reveal new class polymorphic cargo with unique deploy one shot, possibly reducing recipients’ ability defend themselves.

Language: Английский

Citations

0