Transcription Factors ClrB and XlnR and Their Effect on the Transcription of Cellulase Genes in the Filamentous Fungus Penicillium verruculosum DOI Open Access
А. М. Чулкин, V. Yu. Kislitsin, Igor Sinelnikov

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13373 - 13373

Опубликована: Дек. 13, 2024

The filamentous fungus Penicillium verruculosum (anamorph Talaromyces verruculosus) has been shown to be an efficient producer of secreted cellulases, used in biorefinery processes. Understanding the mechanisms regulation cellulase gene expression P. is a current task industrial biotechnology, since it allows for targeted changes composition complex by fungus. Expression genes fungi regulated mainly at level transcription via pathway-specific factors (TF), majority which belong Zn(II)2Cys6 family zinc binuclear cluster proteins. Transcriptional may have species-specific pattern and involves several factors. In this study, we qPCR method transcriptome analysis investigate effect knockouts constitutive encoding homologues regulatory XlnR ClrB from on cbh1, egl2, bgl1 genes, three key cellobiohydrolase, endoglucanase, β-glucosidase, presence various inducers. We that factor strictly responsible main cellulolytic (cbh1, bgl1) xylose xylobiose, but not cellobiose. ClrB/Clr-2, homologue verruculosum, does represent regulating selected inducers, unlike cases Aspergillus nidulans, niger, oxalicum; apparently, different function genus Talaromyces. also resulted 3.5- 2-fold increases activity xylanase β-glucosidase B1-XlnR enzyme preparation, respectively. practical sense, obtained result can production preparations based strain bioconversion renewable cellulose-containing raw materials into technical sugars.

Язык: Английский

Composition of Lignocellulose Hydrolysate in Different Biorefinery Strategies: Nutrients and Inhibitors DOI Creative Commons
Yilan Wang, Yuedong Zhang, Qiu Cui

и другие.

Molecules, Год журнала: 2024, Номер 29(10), С. 2275 - 2275

Опубликована: Май 11, 2024

The hydrolysis and biotransformation of lignocellulose, i.e., biorefinery, can provide human beings with biofuels, bio-based chemicals, materials, is an important technology to solve the fossil energy crisis promote global sustainable development. Biorefinery involves steps such as pretreatment, saccharification, fermentation, researchers have developed a variety biorefinery strategies optimize process reduce costs in recent years. Lignocellulosic hydrolysates are platforms that connect saccharification downstream fermentation. hydrolysate composition closely related biomass raw pretreatment process, choice biorefining strategies, provides not only nutrients but also possible inhibitors for In this review, we summarized effects each stage lignocellulosic on inhibitors, analyzed huge differences nutrient retention inhibitor generation among various emphasized all lignocellulose need be considered comprehensively achieve maximum optimal control at low cost, reference development chemicals.

Язык: Английский

Процитировано

20

Synthetic Microbial Communities Enhance Pepper Growth and Root Morphology by Regulating Rhizosphere Microbial Communities DOI Creative Commons
You Tian, Qiumei Liu, Meng Chen

и другие.

Microorganisms, Год журнала: 2025, Номер 13(1), С. 148 - 148

Опубликована: Янв. 13, 2025

Synthetic microbial community (SynCom) application is efficient in promoting crop yield and soil health. However, few studies have been conducted to enhance pepper growth via modulating rhizosphere communities by SynCom application. This study aimed investigate how inoculation at the seedling stage impacts microbiome using high-throughput sequencing technology. significantly increased shoot height, stem diameter, fresh weight, dry chlorophyll content, leaf number, root vigor, tips, total length, root-specific surface area of 20.9%, 36.33%, 68.84%, 64.34%, 29.65%, 27.78%, 117.42%, 35.4%, 21.52%, 39.76%, respectively, relative control. The Chao index Bray–Curtis dissimilarity fungal increased, while bacterial decreased inoculation. abundances key taxa such as Scedosporium, Sordariomycetes, Pseudarthrobacter, norankSBR1031, norankA4b with inoculation, positively correlated indices growth. Our findings suggest that can effectively regulate morphology regulating increasing abundance like Sordariomycetes thereby benefiting nutrient acquisition, resistance improvement, pathogen crops ensure sustainability.

Язык: Английский

Процитировано

1

Developing a soluble inducer for robust production of cellulases by Trichoderma reesei through submerged fermentation DOI
Xiaoyan Huang, Lin Deng, Xue Zhang

и другие.

Green Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

A novel inducer, ACSM, was developed for cellulases production with Trichoderma reesei , offering greater cost-effectiveness than lactose and microcrystalline cellulose.

Язык: Английский

Процитировано

0

Chitinase Gene FoChi20 in Fusarium oxysporum Reduces Its Pathogenicity and Improves Disease Resistance in Cotton DOI Open Access

Hui Lou,

Jincheng Zhu,

Zengqiang Zhao

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(15), С. 8517 - 8517

Опубликована: Авг. 4, 2024

Chitinase genes, as a class of cell wall hydrolases, are essential for the development and pathogenesis

Язык: Английский

Процитировано

1

A systematic review of abiotic factors influencing the production of plant cell wall-degrading enzymes in Botryosphaeriaceae DOI
Julián D. Restrepo-Leal, Florence Fontaine, Caroline Rémond

и другие.

Fungal Biology Reviews, Год журнала: 2024, Номер 50, С. 100395 - 100395

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

0

Transcription Factors ClrB and XlnR and Their Effect on the Transcription of Cellulase Genes in the Filamentous Fungus Penicillium verruculosum DOI Open Access
А. М. Чулкин, V. Yu. Kislitsin, Igor Sinelnikov

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13373 - 13373

Опубликована: Дек. 13, 2024

The filamentous fungus Penicillium verruculosum (anamorph Talaromyces verruculosus) has been shown to be an efficient producer of secreted cellulases, used in biorefinery processes. Understanding the mechanisms regulation cellulase gene expression P. is a current task industrial biotechnology, since it allows for targeted changes composition complex by fungus. Expression genes fungi regulated mainly at level transcription via pathway-specific factors (TF), majority which belong Zn(II)2Cys6 family zinc binuclear cluster proteins. Transcriptional may have species-specific pattern and involves several factors. In this study, we qPCR method transcriptome analysis investigate effect knockouts constitutive encoding homologues regulatory XlnR ClrB from on cbh1, egl2, bgl1 genes, three key cellobiohydrolase, endoglucanase, β-glucosidase, presence various inducers. We that factor strictly responsible main cellulolytic (cbh1, bgl1) xylose xylobiose, but not cellobiose. ClrB/Clr-2, homologue verruculosum, does represent regulating selected inducers, unlike cases Aspergillus nidulans, niger, oxalicum; apparently, different function genus Talaromyces. also resulted 3.5- 2-fold increases activity xylanase β-glucosidase B1-XlnR enzyme preparation, respectively. practical sense, obtained result can production preparations based strain bioconversion renewable cellulose-containing raw materials into technical sugars.

Язык: Английский

Процитировано

0