High Throughput Parameter Estimation and Uncertainty Analysis Applied to the Production of Mycoprotein from Synthetic Lignocellulosic Hydrolysates DOI Creative Commons
Mason Banks,

Mark Taylor,

Miao Guo

и другие.

Current Research in Food Science, Год журнала: 2024, Номер 9, С. 100908 - 100908

Опубликована: Янв. 1, 2024

The current global food system produces substantial waste and carbon emissions while exacerbating the effects of hunger protein deficiency. This study aims to address these challenges by exploring use lignocellulosic agricultural residues as feedstocks for microbial fermentation, focusing on

Язык: Английский

Co-Hydrothermal Carbonization of Sawdust and Sewage Sludge: Assessing the Potential of the Hydrochar as an Adsorbent and the Ecotoxicity of the Process Water DOI Creative Commons
Matheus Cavali, Thuanne Braúlio Hennig, Nelson Libardi

и другие.

Applied Sciences, Год журнала: 2025, Номер 15(3), С. 1052 - 1052

Опубликована: Янв. 21, 2025

Hydrothermal carbonization (HTC) is a promising thermochemical process to convert residues into hydrochar. While conventional HTC utilizes one type of residue as raw material only, Co-HTC generally combines two. By mixing dry and wet wastes, can advantageously avoid water addition. Therefore, this work investigated the potential hydrochar derived from sawdust non-dewatered sewage sludge dye (methylene blue) adsorbent evaluated toxicity resulting (PW) on Daphnia magna. Three hydrochars were produced by at 180, 215, 250 °C named H-180, H-215, H-250, respectively. For methylene blue adsorption, H-180 H-215 had better performance than H-250. Both presented maximum adsorption capacity approximately 70 mg·g−1, which was superior compared with other in literature. Moreover, removal percentage obtained remained satisfactory even after five cycles. Regarding toxicological assays PWs, raising temperature increased variety substances PW composition, higher D. The EC50 values PW-180, PW-215, PW-250 1.13%, 0.97%, 0.51%, This highlights importance searching for treatment valorization PW. Instead viewing by-product an effluent be treated disposed of, it imperative assess PWs obtaining added-value products.

Язык: Английский

Процитировано

1

Towards the application of nature's catalytic nanomachines: Cellulosomes in 2nd generation biofuel production DOI Creative Commons
Maša Vodovnik,

Nataša Lindič

Biotechnology Advances, Год журнала: 2025, Номер 79, С. 108523 - 108523

Опубликована: Янв. 31, 2025

Язык: Английский

Процитировано

1

Deep Eutectic Solvent Pretreatment and Green Separation of Lignocellulose DOI Creative Commons

Zhengyuan Yao,

Gun Hean Chong, Haixin Guo

и другие.

Applied Sciences, Год журнала: 2024, Номер 14(17), С. 7662 - 7662

Опубликована: Авг. 30, 2024

Plant-based waste biomass with lignocellulose as an important component is produced in large quantities worldwide every year. The components of that typically exhibit high utilization value include cellulose and hemicellulose, well pentoses hexoses derived from their hydrolysis. As a pretreatment for the hydrolysis process, delignification pivotal step to enhance cellulose/hemicellulose accessibility achieve yields fermentable sugars. Additionally, deep eutectic solvents (DESs) are most widely used during fractionation due clean environmentally friendly attributes. DESs dissolve lignin by inducing amount β-O-4 bond cleavage partial carbon–carbon cleavage, retaining solid residue, while hemicellulose hydrolyzed DES pretreatment. This article provides comprehensive review influence separation process. Key factors such removal rate, sugar conversion product chemical structure critically reviewed assess feasibility employing separation.

Язык: Английский

Процитировано

4

Butanol– Product of Agricultural Plant Waste Processing DOI
О. О. Тігунова, Viacheslav Bratishko, Sergіy Shulga

и другие.

Waste and Biomass Valorization, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Язык: Английский

Процитировано

0

High Ectoine Production from Lignocellulosic Hydrolysate by Escherichia coli through Metabolic and Fermentation Engineering DOI

Yifan Feng,

Wenlong Xiao, Xinyi Li

и другие.

ACS Synthetic Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Ectoine, a major compatible solute in halophilic micro-organisms, shows great potential cosmetics and pharmaceuticals areas owing to its water-binding properties capability prevent oxidative damage. In this study, the ectABC gene cluster responsible for ectoine synthesis originated from bacterium Halomonas venusta was first assembled into Escherichia coli. Subsequently, crr PTS knocked out further drive metabolic flux phosphoenolpyruvate oxaloacetate, resulting 1.27 g/L of ectoine. Then, rate-limiting enzyme LysC pathway identified modified. The recombinant E. coli with overexpression feedback-insensitive mutant EclysC* increased titer 2.51 yield 0.37 g/g shake flasks. After medium optimization including carbon nitrogen source, sodium chloride, magnesium sulfate concentration, improved 4.55 g/L. 115.15 0.23 obtained 5.0 L bioreactor through substrate feeding IPTG supplementation fed-batch fermentation. To achieve cost-effective production ectoine, lignocellulosic hydrolysate wheat straw adopted. 134.08 0.33 sugar productivity 3.7 g/L/h finally produced, representing relatively high level renewable resources compared other studies. This study provides valuable insights efficient method industrial-scale production.

Язык: Английский

Процитировано

0

Bioethanol as an alternative fuels: A review on production strategies and technique for analysis DOI Creative Commons
Mona Fatin Syazwanee Mohamed Ghazali, Muskhazli Mustafa

Energy Conversion and Management X, Год журнала: 2025, Номер unknown, С. 100933 - 100933

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Polyhydroxyalkanoate production by engineered Halomonas grown in lignocellulose hydrolysate DOI
Yiping Yuan, Huan Wang,

Hongtao He

и другие.

Bioresource Technology, Год журнала: 2025, Номер 425, С. 132313 - 132313

Опубликована: Фев. 27, 2025

Язык: Английский

Процитировано

0

Optimization and comparative study of Bacillus consortia for cellulolytic potential and cellulase enzyme activity DOI Creative Commons

Ogechukwu Bose Chukwuma,

Mohd Rafatullah,

Riti Thapar Kapoor

и другие.

Open Life Sciences, Год журнала: 2025, Номер 20(1)

Опубликована: Янв. 1, 2025

Lignocellulosic biomass, owing to its recalcitrant nature, requires a consortium of enzymes for breakdown. The present study deals with the isolation cellulolytic bacterial strains from landfill leachate collected Pulau Burung site Penang, Malaysia, and consortia were constructed test their efficiency. dinitro salicylate method was used estimation enzyme activity, compared promising strains. combined potential optimized at varying experimental conditions detect maximum activity. results showed that eight reflected hydrolytic activities, these identified by 16S rDNA sequence as Bacillus subtilis, pumilus, proteolyticus, paramycoides, cereus, altitudinis, niacin, thuringiensis. Consortia A included paramycoides high thermophilic inclination optimal temperature 45°C pH 6 highest cellulase activity 0.90 U/ml. B thuringiensis 0.78 U/ml 38°C 6. significant in breakdown cellulose into useful end products. further proved synergistic relationship more favourable bioconversion processes.

Язык: Английский

Процитировано

0

Mechanism and improvement of yeast tolerance to biomass-derived inhibitors: A review DOI

Jinling Yu,

Chaolun Li,

Y.W. Cheng

и другие.

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108562 - 108562

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Optimization of Squalene Production by Pseudozyma sp. P4-22 DOI Creative Commons
Chen Huang, Xiaojin Song, Jingyi Li

и другие.

Molecules, Год журнала: 2025, Номер 30(7), С. 1646 - 1646

Опубликована: Апрель 7, 2025

Squalene is an important bioactive substance widely used in the food, pharmaceutical, and cosmetic industries. Microbial production of squalene has gained prominence recent years due to its sustainability, safety, environmental friendliness. In this study, a mutant strain, Pseudozyma sp. P4-22, with enhanced squalene-producing ability, was obtained through atmospheric room temperature plasma mutagenesis previously screened yeast SD301. The P4-22 strain demonstrated ability produce using various carbon nitrogen sources. We optimized culture conditions by employing cost-effective corn steep liquor as source, optimal pH sea salt concentration medium were determined be 5.5 5 g/L, respectively. Under cultivation conditions, biomass reached 64.42 g/L 2.06 respectively, L fed-batch fermentation. This study highlights potential promising for commercial-scale squalene.

Язык: Английский

Процитировано

0