Chitosan as a Postharvest Alternative for the Control of Phytophthora capsici in Bell Pepper Fruits

Sci, Год журнала: 2025, Номер 7(2), С. 37 - 37

Опубликована: Март 25, 2025

Phytophthora capsici is a phytopathogen that affects postharvest bell peppers, as it causes rotting and decreases their commercial value. This study evaluates the efficacy of chitosan biocontrol agent against P. on peppers using in vitro vivo assays. The antifungal activity was evaluated at four concentrations (0.5, 1.0, 1.5, 2.0 g L−1). Its effect mycelial growth inhibition, sporangial germination, disease incidence severity, fruit weight loss determined. results show 1.0 L−1 or higher completely inhibited germination reduced severity to 0% treated fruit. Furthermore, treatments preserved fresh dry fruit, which prevented deterioration. demonstrates an effective environmentally friendly alternative for management diseases peppers. could reduce consumer dependence synthetic fungicides preserve quality.

Язык: Английский

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Genome-Wide Identification and Expression Analysis of the Pepper β-1,3-gucanase Gene Family in Response to Phytophthora capsici Stresses

Agronomy, Год журнала: 2025, Номер 15(4), С. 802 - 802

Опубликована: Март 24, 2025

Peppers are widely cultivated around the world, yet they suffer from infections caused by Phytophthora capsici fungi throughout year, which severely impacts their yield. The β-1,3-glucanase gene has been shown in previous studies to significantly enhance plants’ ability cope with both biotic and abiotic stresses, including fungal infections. However, its function peppers not reported. In this study, 80 CaBG genes were initially identified, before being filtered down 54 CaBGs peppers, analyses conducted on physicochemical properties, chromosomal localization, phylogenetic tree relationships, synteny, promoters, expression levels of BG family. results indicated that these located 12 chromosomes. Phylogenetic analysis classified into three subfamilies, α, β, γ, each own specific functions, γ subfamily associated disease resistance peppers. Synteny showed relatively conserved have undergone extensive whole-genome duplication events. Promoter induced plant hormones various external stressors. Transcriptomic RT-qPCR revealed CaBG7 CaBG12 activated following infection P. capsici, two markedly higher resistant plants compared susceptible ones. Based analysis, we propose may be key for chili This study provides a theoretical basis breeding capsici-resistant molecular methods.

Язык: Английский

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Revealing microbial consortia that interfere with grapevine downy mildew through microbiome epidemiology

Environmental Microbiome, Год журнала: 2025, Номер 20(1)

Опубликована: Март 27, 2025

Abstract Background Plant and soil microbiomes can interfere with pathogen life cycles, but their influence on disease epidemiology remains understudied. Here, we analyzed the relationships between plant long-term epidemiological records of grapevine downy mildew, a major caused by oomycete Plasmopara viticola . Results We found that certain microbial taxa were consistently more abundant in plots lower incidence severity community composition could predict severity. Microbial diversity was not strongly linked to records, suggesting is related abundance specific taxa. These key identified topsoil, where pathogen’s oospores overwinter, phyllosphere, zoospores infect leaves. By contrast, leaf endosphere, mycelium develops, contained few interest. Surprisingly, microbiota better predictor than microbiota, microbiome be indicator dynamics this primarily aerial disease. Conclusion Our study integrates data profiles healthy plants reveal fungi bacteria relevant for biocontrol mildew. The resulting database provides valuable resource designing consortia potential activity. framework applied other crop systems guide development strategies reduce pesticide use agriculture.

Язык: Английский

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Biocontrol Effect of Bacillus velezensis D7-8 on Potato Common Scab and Its Complete Genome Sequence Analysis

Microorganisms, Год журнала: 2025, Номер 13(4), С. 770 - 770

Опубликована: Март 28, 2025

Potato common scab, caused by Streptomyces species, is a widespread soil-borne disease that poses significant threat to potato cultivation globally. In this study, Bacillus velezensis D7-8 strain was isolated from potato. This endophytic bacterium exhibited broad-spectrum antifungal activity, and pot trials demonstrated the effectively controlled scab with an efficacy of 42.07%. The complete genome sequence sequenced subsequently identified as B. through multiple bioinformatic methods, primarily structural variation analysis whole-genome sequences. machine learning method predicted expression profiles colinear genes among closely related species were highly consistent. Metabolite crude extracts using ultra-high-performance liquid chromatography coupled quadrupole-Orbitrap high-resolution mass spectrometry (UPLC-Q-Exactive HRMS) revealed produces bioactive compounds, including surfactin fengycin, both known for their antimicrobial properties. study elucidates antagonistic effect against acidiscabies provides valuable reference future research on accurate microbial identification.

Язык: Английский

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Anti-Oomycete Activity and Pepper Root Colonization of Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 against Phytophthora capsici

The Plant Pathology Journal, Год журнала: 2023, Номер 39(1), С. 123 - 135

Опубликована: Фев. 1, 2023

Previously, Pseudomonas plecoglossicida YJR13 and putida YJR92 from a sequential screening procedure were proven to effectively control Phytophthora blight caused by capsici. In this study, we further investigated the anti-oomycete activities of these strains against mycelial growth, zoospore germination, germ tube elongation P. We also root colonization ability bacterial in square dishes, including cell motility (swimming swarming motilities) biofilm formation. Both significantly inhibited growth liquid solid V8 juice media M9 minimal media, compared with Bacillus vallismortis EXTN-1 (positive biocontrol strain), Sphingomonas aquatilis KU408 (negative MgSO4 solution (untreated control). diluted (nutrient-deficient) broth, tested strain populations maintained at >108 cells/ml, simultaneously providing inhibitory activity. Additionally, colonized pepper roots 106 cells/ml concentration for 7 days. The was supported strong swimming activities, formation, chemotactic activity towards exudate components (amino acids, organic sugars) roots. Collectively, results suggest that can suppress through direct germination elongation. Bacterial may be mediated formation together chemotaxis exudates.

Язык: Английский

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Unlocking the Secrets of Rhizosphere Microbes: A New Dimension for Agriculture

Symbiosis, Год журнала: 2024, Номер 92(3), С. 305 - 322

Опубликована: Март 13, 2024

Язык: Английский

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Selection and analysis of the characteristics of Lactiplantibacillus plantarum with antifungal activity

LWT, Год журнала: 2024, Номер 202, С. 116277 - 116277

Опубликована: Июнь 1, 2024

Phytophthora drechsleri is a prevalent spoilage-associated organism found in fruits and vegetables that causes serious economic loss public health concern. Lactiplantibacillus plantarum one of the most promising candidates for use food as fungal antagonist biodetoxification agent. In this study, 347 L. strains were preliminarily evaluated antifungal activity against P. Tucker. Only 5.48% L.plantarum tested exerted strong antagonism IMAU70087 showed greatest inhibitory exhibited broad seven test mold species. Moreover, cell-free supernatant (CFS) thermostability protease resistance. The potential metabolites identified CFS by UPLC/QTOF/MS metabolomics analysis. These mainly organic acids, some which properties. This study provided valuable insights into inhibitor enhancing preservation.

Язык: Английский

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Oomycetes Root Rot Caused by Pythium spp. and Phytophthora spp.: Host Range, Detection, and Management Strategies, Special Case of Olive Trees

Deleted Journal, Год журнала: 2023, Номер 76(1), С. 19 - 47

Опубликована: Ноя. 20, 2023

Root rot is a damaging disease caused by various pathogenic fungi including, Fusarium spp., Rhizoctonia and especially oomycetes. This poses significant challenges to food crop production worldwide. Pythium Phytophthora, most species of these genera, are fungus-like pathogens that can grow expand in diverse agroecosystems, inflicting severe damage the root systems numerous crops, including cereals, vegetables, legumes. Multiple factors contribute proliferation rot, temperature, soil moisture levels, existence vulnerable host plants. Based on wide range scientific literature, this paper examines impact plant safety, emphasizing substantial yield losses economic harm faced farmers The provides also comprehensive overview global prevalence, impact, management strategies associated with infections. A special highlight directed at symptoms, infection process, pathogenicity mechanisms employed Phytophthora species, particular case olive two pathogens. Additionally, detection oomycetes discussed as well, from conventional recent tools now pathology field. Finally, preventive provided work. These include cultural practices, chemical control measures, biological agents, bacteria antagonistic focus use Trichoderma spp. strains, resistance breeding. limitations strategies, such emergence resistant strains environmental concerns, addressed. In conclusion, review helps understand biology, pathogenicity, options for pathogens, which crucial developing sustainable solutions mitigate ensuring security, raising agriculture face challenge.

Язык: Английский

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A review of biocontrol agents in controlling late blight of potatoes and tomatoes caused by Phytophthora infestans and the underlying mechanisms

Pest Management Science, Год журнала: 2023, Номер 79(12), С. 4715 - 4725

Опубликована: Авг. 9, 2023

Phytophthora infestans causes late blight on potatoes and tomatoes, which has a significant economic impact agriculture. The management of been largely dependent the application synthetic fungicides, is not an ultimate solution for sustainable agriculture environmental safety. Biocontrol strategies are expected to be alternative methods conventional chemicals in controlling plant diseases integrated pest (IPM) programs. Well-studied biocontrol agents against include fungi, oomycetes, bacteria, compounds produced by these antagonists, addition certain bioactive metabolites plants. Laboratory glasshouse experiments suggest potential using practical disease management. However, transition field applications problematic moment, due low variable efficacies. In this review, we provide comprehensive summary underlying corresponding mechanisms. To give more intuitive understanding promising agricultural systems, discuss utilizations, modes action future potentials antagonists based their taxonomic classifications. achieve goal best possible results agents, it suggested work trials, strain modifications, formulations, regulations, optimizations application. Combined having different or biological adaptation traits may used strengthen efficacy. More importantly, control should applied coordination other existing forthcoming IPM © 2023 Society Chemical Industry.

Язык: Английский

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The Effects of Antofine on the Morphological and Physiological Characteristics of Phytophthora capsici

Molecules, Год журнала: 2024, Номер 29(9), С. 1965 - 1965

Опубликована: Апрель 25, 2024

Phytophthora capsici is an important plant pathogenic oomycete that causes great losses to vegetable production around the world. Antofine alkaloid isolated from Cynanchum komarovii Al. Iljinski and exhibits significant antifungal activity. In this study, effect of antofine on mycelial growth, morphology, physiological characteristics P. was investigated using colorimetry. Meanwhile, activity mitochondrial respiratory chain complexes evaluated following treatment with a 30% effective concentration (EC30), as well EC50 EC70, for 0, 12, 24, 48 h. The results showed had inhibitory against capsici, 5.0795 μg/mL. After at mycelia were rough, less full, obvious depression; they irregular protrusion structure; serious wrinkles. oxalic acid exopolysaccharide contents decreased significantly, while cell membrane permeability glycerol content increased when treated antofine. Reactive oxygen species (ROS) entered burst state in after incubation 3 h, fluorescence intensity 2.43 times higher than control. activities complex II, III, I + II V, citrate synthase significantly inhibited (EC50 EC70) h compared This study revealed likely affect pathways related energy metabolism thus complexes. These increase our understanding action mechanism capsici.

Язык: Английский

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