Harnessing Streptomyces Biocontrol for Managing Chinese Cabbage Clubroot Disease DOI

Chuokang Chen,

Shan Chen, Qing Wang

и другие.

Опубликована: Янв. 1, 2023

Chinese cabbage clubroot is a globally significant soil-borne disease, inflicting substantial harm to crop yield, quality, and economic value. Biological control offers promising solution, owing its inherent non-toxic, environmentally friendly attributes, resistance drug development. This approach holds immense potential for effective disease management. In this study, we meticulously screened 17 actinomycete strains from pool of 228, identifying two potent with inhibitory rates exceeding 64.0% against Plasmodiophora brassicae infection in cabbage, denoted as XDS3-6 CD1-1. Notably, both CD1-1 were classified within the Streptomyces genus, specifically virginiae S. cinnamonensis, respectively. Furthermore, demonstrated capacity produce proteases glucanases, exhibiting threefold greater protease activity compared XDS3-6. Additionally, triggered activation defense-related pathways, inducing accumulation jasmonic acid (JA) salicylic (SA), along upregulation various resistance-associated enzyme activities. LC-MS non-target profiling unveiled presence diterpene phytoalexins, piperonine, phytocassane E, oxydiol fermentation broths To further enhance our understanding, tracked colonization dynamics mutant plant roots. analysis revealed their remarkable ability colonize roots at least 30 days, peak levels observed on 20th day, reaching 11.0×104 CFU/g 8.5×104 CFU/g, Overall, study highlights biocontrol effectively trigger host which establish robust theoretical foundation application biological strategies managing clubroot.

Язык: Английский

From Lab to Field: Biofertilizers in the 21st Century DOI Creative Commons
Ana Ibáñez, Sonia Garrido-Chamorro, María F. Vasco-Cárdenas

и другие.

Horticulturae, Год журнала: 2023, Номер 9(12), С. 1306 - 1306

Опубликована: Дек. 5, 2023

Nowadays, legal regulations and social environmental concerns are converging towards the promotion of more sustainable agriculture based on organic compounds soil preservation. These trends fuelling growth biofertilizers, which beneficial preparations containing microorganisms able to enhance a plant’s ability uptake essential nutrients. Their production commercialization encompass multitude critical steps deeply reviewed in this manuscript through an exhaustive overview key stages, such as microorganism selection, new sources, upscaling field trials, encapsulation, current application systems regulatory considerations. However, although economical expectations promising, several methodological, environmental, undermining their advancement. The redefinition international frameworks, enhancement trending technologies, fostering multidisciplinary collaboration across sectors players promote biofertilizers eco-friendly cost-effective alternatives chemical fertilizers.

Язык: Английский

Процитировано

23

Evaluation of Pseudomonas koreensis B17-12 as a potential biological control agent against postharvest diseases of tomato DOI

Xi-hong Wei,

Qian-wen Nie,

Rudoviko Galileya Medison

и другие.

Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер 132, С. 102311 - 102311

Опубликована: Май 16, 2024

Язык: Английский

Процитировано

5

An Improved Sporulation Method for Producing Asexual Zoospores of Pythium aphanidermatum on Culture Medium DOI Creative Commons
Yu Jin Kim,

So-Hyeon Baek,

Elena Volynchikova

и другие.

Mycobiology, Год журнала: 2025, Номер 53(1), С. 120 - 127

Опубликована: Янв. 2, 2025

An improved sporulation method for Pythium aphanidermatum on 10% V8-juice agar was developed to generate substantial quantities of zoospores. We conducted experiments using a 4 × factorial design evaluate the effects temperature (20, 25, 30, and 35 °C) incubation time (2, 3, 4, 5 days) zoospore production from isolates HSv05 KACC 48066. The results indicated that 30 °C consistently yielded highest numbers zoospores across all periods. Applying same (30 periods other (CCp03, CCp04, CCp05, HSv10, 40156) also resulted in higher regardless times compared with under control condition (25 2 days). Further virulence tests isolate revealed high concentrations (5 102 - 105 zoospores/ml) induced severe rot symptoms; however, low concentration zoospores/ml produced only considerably weak symptoms inoculated potato tubers. No appeared tubers 0 (uninoculated control) or 101 zoospores/ml. These findings suggest our can help obtain adequate various basic applied studies, including pathogen identification, assessment, resistance resource screening, strategy development.

Язык: Английский

Процитировано

0

Pythium aphanidermatum as a Novel Oomycete Pathogen Causing Leak on Potato ( Solanum tuberosum ) in Korea DOI Creative Commons
Yu Jin Kim, Eui‐Hwan Chung, Ki Deok Kim

и другие.

Mycobiology, Год журнала: 2024, Номер 52(4), С. 222 - 230

Опубликована: Июль 3, 2024

Potato (Solanum tuberosum L.) is one of the most important food crops in Korea. In July 2021 and 2022, dark black-rot symptoms with pink tinges were observed on field-grown potato tubers Hongsung Chuncheon, Korea, respectively. We obtained four isolates (HSv05 HSv10 from Hongsung, CCp03 CCp05 Chuncheon) diseased identified these as Pythium aphanidermatum by analyzing sequences internal transcribed spacer rDNA region mitochondrial cytochrome c oxidase subunit II (COX2) mtDNA gene. Additionally, we compared cultural morphological characteristics those reference isolate KACC 48066 P. literature. Further, tested pathogenicity all against tubers. The test similar to literature; proved pathogenic Therefore, concluded that causal agent leak this first report disease

Язык: Английский

Процитировано

1

Evaluation of biocontrol efficacy of rhizosphere Pseudomonas aeruginosa for management of Phytophthora capsici of pepper DOI Creative Commons

Chenzhen Li,

X. X. Gao,

Yunfeng Huo

и другие.

PLoS ONE, Год журнала: 2024, Номер 19(9), С. e0309705 - e0309705

Опубликована: Сен. 20, 2024

A significant population of biocontrol microorganisms resides in the rhizosphere plants, which can be utilized for plant disease control. To explore potential soil as agents against pepper blight, a bacterial strain Pa608 was screened from and identified Pseudomonas aeruginosa through morphological characteristics 16S rRNA sequences. The result showed that demonstrated antagonistic activity Phytophthora capsici , effectively suppressing mycelial growth. potted experiment high control efficacy 88.0%. Remarkably, also reduced index blight field, resulting efficiencies 74.9%. Moreover, enhanced height yield. GC-MS analysis revealed production numerous secondary metabolites by Pa608, with α-pinene displaying potent anti-oomycete inhibiting P . In conclusion, exhibited management cultivation.

Язык: Английский

Процитировано

1

Complete Genome Resource of a Fungal Antagonistic Strain: Pseudomonas tohonis DOI Creative Commons
Wanzhen Luo, He Zhu,

Hong‐Yue Qi

и другие.

PhytoFrontiers™, Год журнала: 2024, Номер 4(3), С. 453 - 458

Опубликована: Янв. 13, 2024

KRS022 was isolated from the rhizosphere soil of a healthy plant in pathogen-infested cotton field Xinjiang, China. Previous studies have demonstrated that possesses ability to trigger immune responses, inhibit growth various pathogenic fungi, including Verticillium dahliae, and promote growth. The antagonistic properties primarily stem its bioactive metabolites. In this study, high-quality genome obtained. consists chromosome 6,369,026 bp with 67.09% GC content 5,830 open reading frames, which 5,186 genes were annotated, 119 ncRNA genes. Additionally, 11 gene clusters secondary metabolites biosynthesis predicted, involved polyketide synthase, arylpolyene, NAGGN, beta-lactone, ranthipeptide, PiPP-like, redox cofactor, non-ribosomal peptide-synthetase clusters. It could be useful explore biocontrol potential P. tohonis, implying promising application biological control. [Formula: see text] Copyright © 2024 Author(s). This is an access article distributed under CC BY-NC-ND 4.0 International license .

Язык: Английский

Процитировано

0

Pseudomonas plecoglossicida (NR_114226) as a novel biocontrol agent against Fusarium crown rot of wheat DOI
Kamel Eddine Makhlouf,

Boungab Karima,

Slimane Mokrani

и другие.

Journal of Phytopathology, Год журнала: 2024, Номер 172(2)

Опубликована: Март 1, 2024

Abstract Fusarium crown rot (FCR) poses a significant threat to wheat crops, causing substantial grain loss and mycotoxin contamination. While chemical fungicides have been effective in controlling this disease, the need for environmentally friendly alternatives is paramount due concerns about human health fungicide resistance. This study delves into efficacy of Pseudomonas plecoglossicida (NR_114226) as plant growth‐promoting biocontrol agent against graminearum (ON685926) pseudograminearum (ON687723). Greenhouse experiments demonstrated reduction disease severity by 66.06% through seed treatment with rhizobacterial strain soil already infested fungal pathogens. Moreover, growth enhancement occurred F. ‐infected seedlings treated P. (F4 + P57), increasing height from 13.8 33.1 cm root length 9.56 19.62 cm, notable improvements fresh dry weights. The further validated these findings enzymatic assays analysis synthesized secondary metabolites, including chitinase, protease, hydrocyanic acid, indole acetic phosphate solubilization siderophore production. These results strongly suggest that has potential serve an soilborne cereal

Язык: Английский

Процитировано

0

Evaluation of Pseudomonas Koreensis B17-12 as a Potential Biological Control Agent Against Postharvest Diseases of Tomato DOI

Xi hong Wei,

Qian Wen Nie,

Rudoviko Galileya Medison

и другие.

Опубликована: Янв. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Язык: Английский

Процитировано

0

Endophyte Bacillus vallismortis BL01 to Control Fungal and Bacterial Phytopathogens of Tomato (Solanum lycopersicum L.) Plants DOI Creative Commons
Vladimir K. Chebotar, Maria S. Gancheva, Elena P. Chizhevskaya

и другие.

Horticulturae, Год журнала: 2024, Номер 10(10), С. 1095 - 1095

Опубликована: Окт. 14, 2024

Some strains of Bacillus vallismortis have been reported to be efficient biocontrol agents against tomato pathogens. The aim our study was assess the ability endophytic strain BL01 through in vitro and field trials, as well verify its plant colonization analyze bacterial genome order find genes responsible for activity. We demonstrated a gnotobiotic system by confocal laser microscopy that able colonize endosphere rhizosphere tomato, winter wheat oilseed rape. In experiments inhibition activity wide range phytopathogenic fungi bacteria. showed biological efficacy two-year with plants black spotting 40–70.8% late blight 47.1% increased harvest 24.9% or 10.9 tons per hectare compared control. Genome analysis revealed presence are synthesis biologically active secondary metabolites, which could action. Strain B. can considered an effective agent control both fungal diseases plants.

Язык: Английский

Процитировано

0

Harnessing Streptomyces Biocontrol for Managing Chinese Cabbage Clubroot Disease DOI

Chuokang Chen,

Shan Chen, Qing Wang

и другие.

Опубликована: Янв. 1, 2023

Chinese cabbage clubroot is a globally significant soil-borne disease, inflicting substantial harm to crop yield, quality, and economic value. Biological control offers promising solution, owing its inherent non-toxic, environmentally friendly attributes, resistance drug development. This approach holds immense potential for effective disease management. In this study, we meticulously screened 17 actinomycete strains from pool of 228, identifying two potent with inhibitory rates exceeding 64.0% against Plasmodiophora brassicae infection in cabbage, denoted as XDS3-6 CD1-1. Notably, both CD1-1 were classified within the Streptomyces genus, specifically virginiae S. cinnamonensis, respectively. Furthermore, demonstrated capacity produce proteases glucanases, exhibiting threefold greater protease activity compared XDS3-6. Additionally, triggered activation defense-related pathways, inducing accumulation jasmonic acid (JA) salicylic (SA), along upregulation various resistance-associated enzyme activities. LC-MS non-target profiling unveiled presence diterpene phytoalexins, piperonine, phytocassane E, oxydiol fermentation broths To further enhance our understanding, tracked colonization dynamics mutant plant roots. analysis revealed their remarkable ability colonize roots at least 30 days, peak levels observed on 20th day, reaching 11.0×104 CFU/g 8.5×104 CFU/g, Overall, study highlights biocontrol effectively trigger host which establish robust theoretical foundation application biological strategies managing clubroot.

Язык: Английский

Процитировано

0