Immune responses to human fungal pathogens and therapeutic prospects

Nature reviews. Immunology, Год журнала: 2023, Номер 23(7), С. 433 - 452

Опубликована: Янв. 4, 2023

Язык: Английский

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Antifungal drug-resistance mechanisms in Candida biofilms

Current Opinion in Microbiology, Год журнала: 2022, Номер 71, С. 102237 - 102237

Опубликована: Ноя. 25, 2022

Infections caused by the Candida species of human fungal pathogens are a significant medical problem because they can disseminate to nearly every organ body. In addition, there only few classes antifungal drugs available treat patients with invasive infections. infections that associated biofilms withstand much higher concentrations compared planktonic cells, thus making biofilm particularly challenging treat. albicans is among most prevalent microbiota, asymptomatically colonizing several niches body, including gastrointestinal tract, genitourinary mouth, and skin. Immunocompromised health conditions, dysbiosis or environmental changes, however, lead C. overgrowth, causing range from superficial mucosal severe hematogenously disseminated Here, we review current knowledge drug-resistance mechanisms occurring in biofilms.

Язык: Английский

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Invasive candidiasis

Nature Reviews Disease Primers, Год журнала: 2024, Номер 10(1)

Опубликована: Март 21, 2024

Язык: Английский

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C5a-licensed phagocytes drive sterilizing immunity during systemic fungal infection

Cell, Год журнала: 2023, Номер 186(13), С. 2802 - 2822.e22

Опубликована: Май 22, 2023

Язык: Английский

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A Critical Review on Sustainable Formulation of Anti-quorum Sensing Compounds Using Nanotechnology Principles Against Candida albicans

BioNanoScience, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 2, 2025

Язык: Английский

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DSF-family quorum sensing signal-mediated intraspecies, interspecies, and inter-kingdom communication

Trends in Microbiology, Год журнала: 2022, Номер 31(1), С. 36 - 50

Опубликована: Авг. 5, 2022

DSFs are cis-2-unsaturated fatty acids containing acid carbon chains of various lengths and cis double-bond configurations. DSF-producing bacteria widely distributed in nature.Based on their genomic origins, DSF-mediated intraspecies communication pathways generally affiliated to three different groups, respectively represented by Xanthomonas campestris pv. (Xcc), Burkholderia cenocepacia (Bcc), Pseudomonas aeruginosa.DSF-mediated interspecies occurs between B. cenocepacia, Stenotrophomonas maltophilia, Bacillus, Francisella novicida, Salmonella, Bdellovibrio bacteriovorus.DSF-mediated inter-kingdom Candida albicans.DSF signaling plant development defense, Xylella fastidiosa colonization insects, the zebrafish inflammatory response. While most unicellular microbes they communicate with each other environments adapt behaviors. Quorum sensing (QS) is one best-studied cell–cell modes. QS not restricted bacterial cell-to-cell – it also allows eukaryotic hosts. The diffusible signal factor (DSF) family represents an intriguing type multiple roles found diverse Gram-negative bacteria. Over last decade, extensive progress has been made understanding among bacteria, fungi, plants, zebrafish. This review provides update these new developments aim building a more comprehensive picture intraspecies, interspecies, communication. Microbes ubiquitous populate plants animals, as well soil, water, air. They form communities multitude environments. It becoming increasingly apparent that isolated specific but rather interact surroundings [1.Thompson L.R. et al.The Earth microbiome project consortium. A communal catalogue reveals Earth's multiscale microbial diversity.Nature. 2017; 551: 457-463Crossref PubMed Scopus (1204) Google Scholar]. One key challenges microbiology dissect diversity, metabolic activities, interactions within community associated (see Glossary), process whereby monitor population density regulate gene expression using chemical signals, [2.Leoni L. al.Leoni Rampioni G. Sensing Methods Protocols. Humana Press, 2017: 1-4Google widespread eubacteria, archaea, fungi. Research recent decades identified rich inventory signals QS-regulated beneficial or competitive Recent evidence shows limited hosts [3.Hughes D. al.Inter-kingdom signaling: hosts.Nat. Rev. Microbiol. 2008; 6: 111-120Crossref (550) Scholar, 4.Hartmann A. al.Importance N-acyl-homoserine lactone-based quorum quenching pathogen control growth promotion.Pathogens. 2021; 10: 1561Crossref (8) 5.Fan Q. al.Structure regulation mechanism interkingdom system receptors.J. Agric. Food Chem. 2022; 70: 429-445Crossref (7) Understanding mechanisms outcomes signal-mediated important implications for appreciating host–pathogen and, ultimately, may provide targets strategies antimicrobial therapies block interfere networks. [6.Deng Y. al.Listening language: DSF-based bacteria.Chem. 2011; 111: 160-173Crossref (191) Scholar,7.Zhou DSF signals: biosynthesis, turnover Xanthomonas.Trends 25: 293-303Abstract Full Text PDF (121) Recently, reported [8.Tran T.M. hijacks Arabidopsis thaliana sterol biosynthesis suppress innate immunity.Life Sci. Alliance. 2020; 3e202000720Crossref 9.Ma Z. al.Formin nanoclustering-mediated actin assembly during flagellin signaling.Cell Rep. 34108884Abstract (15) 10.Zhu H. al.Bacterial inhibits LPS-induced inflammations suppressing Toll-like receptor preventing lysosome mediated apoptosis zebrafish.Int. J. Mol. 23: 7110Crossref (1) More detailed descriptions structures, biological functions, can be previous reviews Scholar,11.He Y.W. al.Quorum virulence campestris.FEMS 32: 842-857Crossref (200) 12.Dow J.M. Diffusible factor-dependent pathogenic its exploitation disease control.J. Appl. 2016; 122: 2-11Crossref (32) 13.Wang M. cis-2-dodecenoic (BDSF) cenocepacia.Appl. Environ. 88e0234221Crossref (4) For convenience, will used collective term DSF-family unless otherwise specified. first discovered was cis-11-methyl-dodecenoic (XcDSF) phytopathogen (Xcc). Subsequently, several analogs, (BDSF), cis, cis-11-methyldodeca-2,5-dienoic (CDSF), cis-10-methyl-2-dodecenoic (IDSF), 13-methyltetradecanoic (LeDSF), cis-2-tetradecenoic (XfDSF1), cis-2-hexadecenoic (XfDSF2), cis-2-decenoic (PDSF), have identified. Except LeDSF, all configurations (Figure 1). Until now, members detected range species belonging γ-proteobacteria β-proteobacteria including spp., fastidiosa, Lysobacter enzymogenes brunescens, Leptospirillum ferri philum ferrooxidans, Cronobacter turicensis, aeruginosa [7.Zhou Scholar,14.An S.Q. al.Diffusible regulates functions opportunistic maltophilia.BMC Res. Notes. 2018; 11: 569Crossref (16) 15.Li (DSF)-mediated modulates traits citri responses citrus promote disease.BMC Genomics. 2019; 20: 55Crossref (24) 16.Feng T. al.Interspecies synergistically twitching motility.Appl. 85e01742Crossref (12) 17.Ling al.LbDSF, brunescens quorum-sensing factor, anti- XSAC colony morphology, surface motility.Front. 1230Crossref 18.Alcaraz E. rpf/DSF signalling maltophilia positively biofilm formation, production virulence-associated factors β-lactamase induction.FEMS Lett. 366fnz069Crossref (18) 19.Yero al.Genetic variants influence resistance phenotypes genotypically clinical isolates.Front. 1160Crossref (11) 20.Bellenberg S. controls bioleaching activity niche protection acidophilic, mineral-oxidizing Leptospirilli.Sci. 16275Crossref (5) 21.Singh P. synthase, RpfF, oryzae required maintenance membrane integrity virulence.Mol. Plant Pathol. 118-132Crossref In silico analysis suggests Methylobacillus flagellates, Thiobacillus denitrificans, genera Frateuria, Luteibacter, Pseudoxanthomonas, Rhodanobacter produce DSF. By definition, individuals same species. helps sense size degree confinement, allowing them function multicellular organism execute broad Based Xcc, aeruginosa. distinguished presence 'regulation pathogenicity factors' (rpf) cluster encoding components such synthase RpfF perception RpfC/RpfG functionally verified phytopathogens X. strains environmental relevance spp. [14.An Scholar,16.Feng Bacteria this through classic synthesis pathway final bifunctional enzyme, having both dehydratase thioesterase activities [22.Zhou synthesized from carbohydrate branched-chain amino via FAS elongation cycle.Sci. 2015; 5: 13294Crossref (55) However, strictly regulated growth. whole QS-dependent comprises stages: pre-QS, QS, post-QS, Xcc At pre-QS stage, cell density-dependent autoinduced protein–protein interaction mechanism. sensor RpfC forms complex receiver domain [23.He al.Dual hybrid kinase involve either phosphorelay domain–protein interaction.J. Biol. 2006; 281: 33414-33421Abstract (99) post-QS RpfB-dependent induced levels return those stage [24.Zhou al.Identification characterization naturally occurring Xanthomonas.Environ. 17: 4646-4658Crossref (41) Scholar] 2A ). perceived transduced two-component comprising response regulator RpfG. activated HD-GYP RpfG phosphodiesterase degrade second messenger cyclic-di-GMP, ligand global cyclic AMP receptor-like protein (Clp), which, turn, directly indirectly [25.He al.Xanthomonas cell-cell involves putative nucleotide Clp hierarchical network.Mol. 2007; 64: 281-292Crossref (157) Scholar,26.Tao F. monophosphate defines class di-GMP effectors.J. Bacteriol. 2010; 192: 1020-1029Crossref (148) Activated shown two GGDEF domain-containing proteins motility [27.Ryan R.P. al.Cell-cell signal-dependent dynamic mediate campestris.Proc. Natl. Acad. U. 107: 5989-5994Crossref (117) addition, negatively aggregate ABC (xagABC)-dependent formation [28.Tao modulation glycosyltransferase essential formation.Environ. 12: 3159-3170Crossref (60) 2B). Interestingly, atypical RpfF/RpfC-regulated were (Xoo) strain BXO43 [29.Rai R. promotes ferric iron uptake oryzicola contribute inside rice.Mol. 96: 708-727Crossref (20) Scholar,30.Ionescu al.Promiscuous responsiveness rpf System.mBio. 7e01054Crossref (31) These findings suggest category I might than what current models suggest. pathogens, biofilm, multidrug resistance, integrity, [15.Li Scholar,21.Singh Scholar,31.He al.Genome scale regulon campestris: identification novel communication-dependent genes functions.Mol. 59: 610-622Crossref (150) Scholar,32.Barel V. al.Virulence planta movement hortorum pelargonii affected (DSF)-dependent system.Mol. 16: 710-723Crossref (13) soil bacterium Lysobacter, LeDSF antibiotics pigment, [16.Feng Scholar,17.Ling acidophilic Leptospirilli [20.Bellenberg characterized rpfF homolog rpfFBC rpfR, BDSF cepacia (Bcc) genus [33.Deng al.Structural functional produced complex.Appl. 76: 4675-4683Crossref (95) Scholar,34.Suppiger al.Evidence molecules aid biosensors.Environ. 8: 38-44Crossref major II system, RqpS/RqpR, modulating rpfFBc transcription [35.Cui C. al.A cenocepacia.Mol. 108: 32-44Crossref (26) recently reviewed [13.Wang RpfR Per/Arnt/Sim (PAS), GGDEF, EAL domain. binds PAS RpfR, causing conformation change. change plays critical role regulating thereby influencing intracellular cyclic-di-GMP [36.Waldron E.J. basis recognition regulatory RpfF.PLoS 17e3000123Crossref able bind [34.Suppiger regulator, GtrR, interacts cepI, acyl homoserine lactone (AHL)-type [37.Yang al.Burkholderia integrates dimeric guanosine virulence.Proc. 114: 13006-13011Crossref (43) link AHL-dependent underlining complexity signaling. drug-resistant produces DSF-type molecule PDSF. PDSF attributed dspI (PA0745), located PA0743–PA0747 PAO1 [38.Amari D.T. enoyl-coenzyme hydratase DspI dispersion autoinducer acid.J. 2013; 195: 4600-4610Crossref (46) another cluster, PA4978–PA4983, suggested involved [39.Rahmani-Badi al.Dissection network microarray technique.Front. 383PubMed induce PA14 Microarray revealed dispersion, motility, virulence, persistence PA14, PDSF-deficiency provoked remarkable reduction pyoverdine production, swarming [40.Liu studies DspI: biosynthesis.Sci. 3928Crossref (19) transduction remains elucidated. Given PDSF-regulated mostly overlap signaling, likely never live alone natural ecosystems physically trophically other. detect only self-produced neighbors [41.Coquant al.(2020) Impact lactones, molecules, gut immunity.Front. Immunol. 1827; 2020: 11Google prodigious diversity nature, surprising enormous repertoire occurs. aeruginosa, multidrug-resistant pathogens. Chronic infection occur 80% cystic fibrosis (CF) patients, who often coinfected fungus Aspergillus sp. [42.Hughes D.A. al.Clinical characteristics co-infected patients: UK registry study.J. Cyst. Fibros. 21: 129-135Abstract As described previously, PDSF, BDSF, XcDSF, respectively, mediate, order, III, II, maltophilia–P. cocultures, influences architecture biofilm. requires PA1396 [43.Ryan polymyxin tolerance aeruginosa.Mol. 68: 75-86Crossref (179) Mutation exogenous addition XcDSF results increased stress suggesting sensed Further, at physiologically relevant concentrations sputum samples CF patients infected Bcc [44.Twomey K.B. airway modulate aeruginosa.ISME 2012; 939-950Crossref (78) correlated polymicrobial infections involving and/or together When inoculated intratracheally into transmembrane conductance (CFTR) knockout mice, observed lung Furthermore, could biofilms human CF-derived epithelial cells vitro culture system. Addition significantly enhanced biofilms. [45.Deng al.Cis-2-dodecenoic interference systems T3SS.BMC 13: 231Crossref Exogenous decreases transcriptional level lasR, rhlR, pqsR leading reduced AHL quinolone (PQS) III secretion (T3SS) micromolar reduces HeLa models. PA1396, long-chain PsrA. Instead, appeared inhibit T3SS alternative independent Taken together, clearly operate lungs

Язык: Английский

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Virulence Factors of Candida spp. and Host Immune Response Important in the Pathogenesis of Vulvovaginal Candidiasis

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(11), С. 5895 - 5895

Опубликована: Май 24, 2022

Vulvovaginal candidiasis (VVC) is one of the most common types vaginal infections in women around world and often underestimated by both patients doctors. Research on pathogenesis fungal over last 20 years has resulted a closer understanding virulence factors involved Candida epithelial invasion their mechanisms action. Recently, attention was drawn to enormous complexity interaction between yeast-like fungi host cells, as well level host’s response infection impact course treatment VVC. Our work provides broad description already known some new reports (such phenotypic switching or biofilm formation capacity) importance for tissue At same time, we also focus interactions with cells local innate immune that are now considered equally important this case. The presented review describes aspects still unknown pathogenicity associated infections.

Язык: Английский

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Limonene inhibits virulence associated traits in Candida albicans: In-vitro and in-silico studies

Phytomedicine Plus, Год журнала: 2022, Номер 2(3), С. 100285 - 100285

Опубликована: Апрель 19, 2022

: Multidrug resistance and undesirable side effects of currently available antifungal drugs, has made treatment Candidiasis difficult in immunocompromised patients. Limonene, commonly found citrus essential oils, immense therapeutic potential. Efficacy this monoterpene was studied against virulence attributes C. albicans. Percentage haemolysis, susceptibility, time kill studies, secretion hydrolytic enzymes, morphological transition, adhesion biofilm formation (XTT reduction assay, scanning electron microscopic calcofluor white stained fluorescence imaging) were the presence limonene. Molecular docking by InstaDock software followed MD simulation studies (AMBER suite) assessed limonene-protein interactions with five associated targets (Als3, Bcr1, Plb1, Sap2 Tec1). With MIC MFC values 300 µg/mL 400 µg/mL, respectively, it caused only 1% haemolysis (3 times less than fluconazole) significantly reduced to buccal epithelial cells. An incubation 9 h at 2MIC growth 100%. At MIC, proteinases phospholipases 73% 53%, respectively. Adhesion 91% 87%, Biofilm biomass formed on silicon sheets inhibited 69%. Limonene disrupted structure integrity. Filamentation, four different hyphae inducing media, reduced. Although stable hydrophobic observed all target proteins, limonene gave a good score Plb1 (−6.0 kcal/mol) Tec1 (−5.7 kcal/mol). Complexes both these proteins showed RMSD profiles except Bcr1. Conclusion: binds critical Candida pathogenicity inhibits adhesion, transition. It is nontoxic potential management invasive candidiasis.

Язык: Английский

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The Interplay between Candida albicans, Vaginal Mucosa, Host Immunity and Resident Microbiota in Health and Disease: An Overview and Future Perspectives

Microorganisms, Год журнала: 2023, Номер 11(5), С. 1211 - 1211

Опубликована: Май 5, 2023

Vulvovaginal candidiasis (VVC), which is primarily caused by Candida albicans, an infection that affects up to 75% of all reproductive-age women worldwide. Recurrent VVC (RVVC) defined as >3 episodes per year and nearly 8% globally. At mucosal sites the vagina, a delicate complex balance exists between spp., host immunity local microbial communities. In fact, both immune response microbiota composition play central role in counteracting overgrowth fungus maintaining homeostasis host. If this perturbed, conditions may favor C. albicans yeast-to-hyphal transition, predisposing VVC. To date, factors affect equilibrium spp. drive transition from commensalism pathogenicity are not yet fully understood. Understanding host- fungus-related pathogenesis paramount importance for development adequate therapeutic interventions combat common genital infection. This review focuses on latest advances pathogenic mechanisms implicated onset also discusses novel potential strategies, with special focus use probiotics vaginal transplantation treatment and/or prevention recurrent

Язык: Английский

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Roles of Streptococcus mutans-Candida albicans interaction in early childhood caries: a literature review

Frontiers in Cellular and Infection Microbiology, Год журнала: 2023, Номер 13

Опубликована: Май 16, 2023

As one of the most common oral diseases in kids, early childhood caries affects health children throughout world. Clinical investigations show copresence Candida albicans and Streptococcus mutans ECC lesions, mechanistic studies reveal co-existence C. S. both their cariogenicity. Clearly a comprehensive understanding interkingdom interaction between these two microorganisms has important implications for treatment prevention. To this end, review summarizes advances our virulence . More importantly, synergistic antagonistic interactions microbes are discussed.

Язык: Английский

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