Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Окт. 9, 2023
CRISPR-Cas9,
the
“genetic
scissors”,
is
being
presaged
as
a
revolutionary
technology,
having
tremendous
potential
to
create
designer
crops
by
introducing
precise
and
targeted
modifications
in
genome
achieve
global
food
security
face
of
climate
change
increasing
population.
Traditional
genetic
engineering
relies
on
random
unpredictable
insertion
isolated
genes
or
foreign
DNA
elements
into
plant
genome.
However,
CRISPR-Cas
based
gene
editing
does
not
necessarily
involve
inserting
element
from
different
species
but
new
traits
precisely
altering
existing
genes.
CRISPR
edited
are
touching
markets,
however,
world
community
divided
over
whether
these
should
be
considered
genetically
modified
(GM)
non-GM.
Classification
crops,
especially
transgene
free
traditional
GM
will
significantly
affect
their
future
public
acceptance
some
regions.
Therefore,
depending
upon
regulation
non-GMs,
perception.
Here
we
briefly
discuss
how
crops.
In
addition,
reagents
delivery
tools
produce
transgene-free
Moreover,
also
summarize
regulatory
classification
countries
regulating
We
that
controversy
CRISPR-edited
plants
non-GM
continue
until
universal,
transparent,
scalable
framework
for
introduced
worldwide,
with
increased
awareness
involving
all
stakeholders.
Biotechnology for Biofuels and Bioproducts,
Год журнала:
2024,
Номер
17(1)
Опубликована: Янв. 23, 2024
Abstract
The
development
of
microalgal
biofuels
is
significant
importance
in
advancing
the
energy
transition,
alleviating
food
pressure,
preserving
natural
environment,
and
addressing
climate
change.
Numerous
countries
regions
across
globe
have
conducted
extensive
research
strategic
planning
on
bioenergy,
investing
funds
manpower
into
this
field.
However,
microalgae
biofuel
industry
has
faced
a
downturn
due
to
constraints
high
costs.
In
past
decade,
with
new
strains,
technologies,
equipment,
feasibility
large-scale
production
should
be
re-evaluated.
Here,
we
gathered
results
from
decade
regarding
production,
providing
insights
opportunities
challenges
by
perspectives
selection,
modification,
cultivation.
review,
suggest
that
highly
adaptable
are
preferred
choice
for
especially
strains
can
utilize
concentrations
inorganic
carbon
sources
possess
stress
resistance.
use
omics
technologies
genetic
editing
greatly
enhanced
lipid
accumulation
microalgae.
associated
risks
constrained
outdoor
Therefore,
relatively
controllable
cultivation
method
photobioreactors
(PBRs)
made
it
mainstream
approach
production.
Moreover,
adjusting
performance
parameters
PBRs
also
enhance
future,
given
relentless
escalation
demand
sustainable
sources,
deemed
pivotal
constituent
national
planning,
particularly
case
China.
advancement
synthetic
biology
helps
reduce
genetically
modified
(GM)
enhances
economic
viability
their
Graphical
GM crops & food,
Год журнала:
2017,
Номер
8(1), С. 1 - 12
Опубликована: Янв. 2, 2017
Genome
editing
of
crop
plants
is
a
rapidly
advancing
technology
whereby
targeted
mutations
can
be
introduced
into
plant
genome
in
highly
specific
manner
and
with
great
precision.
For
the
most
part,
does
not
incorporate
transgenic
modifications
far
superior
to
conventional
chemical
mutagenesis.
In
this
study
we
bring
focus
some
underlying
differences
between
3
existing
technologies:
classical
breeding,
genetic
modification
editing.
We
discuss
main
achievements
from
each
area
highlight
their
common
characteristics
individual
limitations,
while
emphasizing
unique
capabilities
subsequently
examine
possible
regulatory
mechanisms
which
governments
may
inclined
use
assessing
status
edited
products.
If
assessed
on
basis
phenotype
rather
than
process
by
they
are
obtained,
these
products
will
categorized
as
equivalent
those
produced
This
would
mean
that
subject
restrictions
imposed
genetically
modified
products,
except
cases
where
mutation
involves
large
sequence
insertion
genome.
conclude
examining
potential
societal
acceptance
technology,
reinforced
scientific
perspective
promoting
such
acceptance.
New Phytologist,
Год журнала:
2018,
Номер
222(4), С. 1673 - 1684
Опубликована: Дек. 13, 2018
Summary
A
special
regulatory
regime
applies
to
products
of
recombinant
nucleic
acid
modifications.
ruling
from
the
European
Court
Justice
has
interpreted
this
in
a
way
that
it
also
emerging
mutagenesis
techniques.
Elsewhere
progress
is
ongoing.
In
2015,
Argentina
launched
framework,
followed
by
Chile
2017
and
recently
Brazil
Colombia.
March
2018,
USDA
announced
will
not
regulate
genome‐edited
plants
differently
if
they
could
have
been
developed
through
traditional
breeding.
Canada
an
altogether
different
approach
with
their
Plants
Novel
Traits
regulations.
Australia
currently
reviewing
its
Gene
Technology
Act.
This
article
illustrates
deviation
Union's
(EU's)
one
most
other
countries
studied
here.
Whereas
EU
does
implement
case‐by‐case
approach,
taken
several
jurisdictions.
Also,
court
adheres
process‐based
while
stronger
emphasis
on
regulation
resulting
product.
It
concluded
that,
unless
functioning
identity
preservation
system
for
directed
can
be
established,
results
risk
asynchronous
approvals
disruptions
international
trade.
Agronomy,
Год журнала:
2018,
Номер
8(7), С. 128 - 128
Опубликована: Июль 23, 2018
The
astonishing
increase
in
temperature
presents
an
alarming
threat
to
crop
production
worldwide.
As
evident
by
huge
yield
decline
various
crops,
the
escalating
drastic
impacts
of
heat
stress
(HS)
are
putting
global
food
as
well
nutritional
security
at
high
risk.
HS
is
a
major
abiotic
that
influences
plant
morphology,
physiology,
reproduction,
and
productivity
physiological
molecular
responses
dynamic
research
areas,
techniques
being
adopted
for
producing
tolerant
plants.
In
this
article,
we
reviewed
recent
findings,
impacts,
adoption,
tolerance
cellular,
organellar,
whole
level
reported
several
approaches
used
improve
Omics
unravel
mechanisms
underlying
thermotolerance,
which
imperative
understand
processes
toward
HS.
Our
review
about
may
enlighten
ways
develop
thermo-tolerant
cultivars
produce
plants
agriculturally
important
adverse
climatic
conditions.
Transgenic Research,
Год журнала:
2019,
Номер
28(2), С. 247 - 256
Опубликована: Март 4, 2019
Innovation
in
agriculture
is
pervasive.
However,
spite
of
the
success
stories
twentieth
century
plant
breeding,
twenty-first
has
ushered
a
set
challenges
that
solutions
from
past
are
unlikely
to
address.
sustained
research
and
amalgamation
number
disciplines
resulted
new
breeding
techniques
(NBTs),
such
as
genome
editing,
which
offer
promise
opportunities
resolve
some
issues.
Here
we
present
results
an
expert
survey
on
added
potential
benefits
genome-edited
crops
compared
those
developed
through
genetic
modification
(GM)
conventional
breeding.
Overall,
reveal
consensus
among
experts
enhanced
agronomic
performance
product
quality
over
alternatives.
The
majority
indicated
regulations
for
health
safety,
followed
by
export
markets,
consumers,
media
play
major
role
determining
where
how
NBTs,
including
will
be
used
agriculture.
Further
needed
gauge
opinion
after
Court
Justice
European
Union
ruling
establishing
site-specific
mutagenic
technologies
regulated
same
fashion
GM
crops,
regardless
whether
foreign
DNA
final
variety.
Two-line
hybrid
rice
with
high
yield
potential
is
increasingly
popular
and
the
photo-
temperature-sensitive
male
sterile
line
one
of
basic
components
for
two-line
breeding.
The
development
lines
through
conventional
breeding
a
lengthy
laborious
process,
whereas
developing
thermo-sensitive
genic
(TGMS)
by
editing
temperature-sensitivity
gene
CRISPR/Cas9
efficient
convenient.
Here,
sterility
was
induced
employing
technology
to
modify
TMS5.
Two
TGMS
mutants,
tms5–1
tms5–2,
both
lacking
any
residual
T-DNA,
were
generated
in
indica
cultivar
Zhongjiazao17
(cv.
YK17)
background.
When
grown
at
sub-optimal
temperature
(22
°C),
mutants
produced
viable
pollen
successfully
grain
self-fertilization,
but
temperatures
24
26
°C,
their
no
set.
F1
hybrids
derived
from
crosses
between
YK17S
(tms5–1)
three
different
restorer
outperformed
parental
respect
related
traits.
system
proved
be
new
superior
can
widely
utilized
rice.
Abstract
The
current
pace
of
crop
improvement
is
inadequate
to
feed
the
burgeoning
human
population
by
2050.
Higher,
more
stable,
and
sustainable
production
required
against
a
backdrop
drought
stress,
which
causes
significant
losses
in
yields.
Tailoring
crops
for
adaptation
may
hold
key
address
these
challenges
provide
resilient
systems
future
harvests.
Understanding
genetic
molecular
landscape
functionality
alleles
associated
with
adaptive
traits
will
make
designer
breeding
prospective
approach
improvement.
Here,
we
highlight
potential
genomics
technologies
combined
physiology
high‐throughput
identification
architecture
drought‐adaptive
explore
innovative
genomic
strategies
designing
crops.