Опубликована: Янв. 1, 2025
Язык: Английский
Agronomy, Год журнала: 2021, Номер 11(11), С. 2188 - 2188
Опубликована: Окт. 29, 2021
Tomato (Solanum lycopersicum L.), family Solanaceae, has become in the past fifty years one of most important and extensively grown horticultural crops Mediterranean region throughout world. In 2019, more than 180 million tonnes tomato have been produced worldwide, out which around 42 countries. Due to its genetic properties, is afflicted by numerous plant diseases induced fungal, bacterial, phytoplasma, virus, viroid pathogens. Not only inheritance great importance management pathogens, but equally as are also present climate changes, recently revised phytopathological control measures, globalization seed industry. Thus, recognition symptoms knowledge distribution spread disease methods for early detection pathogens major prerequisites a successful disease. this review, we will describe main area that impact mostly yield provide current perspective measures necessary their management.
Язык: Английский
Процитировано
208
The Crop Journal, Год журнала: 2021, Номер 10(1), С. 13 - 25
Опубликована: Апрель 6, 2021
Crop yield loss due to soil salinization is an increasing threat agriculture worldwide. Salt stress drastically affects the growth, development, and grain productivity of rice (Oryza sativa L.), improvement tolerance salt a desirable approach for meeting food demand. The main contributors toxicity at global scale are Na+ Cl− ions, which affect up 50% irrigated soils. Plant responses occur organismic, cellular, molecular levels pleiotropic, involving (1) maintenance ionic homeostasis, (2) osmotic adjustment, (3) ROS scavenging, (4) nutritional balance. In this review, we discuss recent research progress on these four aspects plant physiological response, with particular attention hormonal gene expression regulation signaling pathways in rice. information summarized here will be useful accelerating breeding salt-tolerant
Язык: Английский
Процитировано
201
International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(4), С. 1878 - 1878
Опубликована: Фев. 13, 2021
Tomato is one of the major vegetable crops consumed worldwide. yellow leaf curl virus (TYLCV) and fungal Oidium sp. are devastating pathogens causing disease powdery mildew. Such viral reduce tomato crop yields cause substantial economic losses every year. Several commercial varieties include Ty-5 (SlPelo) Mildew resistance locus o 1 (SlMlo1) that carries susceptibility (S-gene) factors for TYLCV mildew, respectively. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) a valuable genome editing tool to develop disease-resistant varieties. In this regard, targeting encoded by host plant instead promising approach achieve pathogen without need stable inheritance CRISPR components. study, CRISPR/Cas9 system was employed target SlPelo SlMlo1 trait introgression in elite cultivar BN-86 confer host-mediated immunity against pathogens. SlPelo-knockout lines were successfully generated, carrying biallelic indel mutations. assays mutant confirmed suppressed accumulation restricted spread non-inoculated parts. Generated knockout showed complete mildew fungus. Overall, our results demonstrate efficiency introduce targeted mutagenesis rapid development pathogen-resistant tomato.
Язык: Английский
Процитировано
113
International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 12053 - 12053
Опубликована: Окт. 10, 2022
The major challenges that agriculture is facing in the twenty-first century are increasing droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate change. However, most crops not tolerant climatic environments. aim near future, a world with hunger an population, breed and/or engineer tolerate abiotic stress higher yield. Some crop varieties display certain degree of tolerance, which has been exploited by plant breeders develop thrive under conditions. Moreover, long list genes involved tolerance have identified characterized molecular techniques overexpressed individually transformation experiments. Nevertheless, phenotypes polygenetic traits, current genomic tools dissecting exploit their use accelerating genetic introgression using markers or site-directed mutagenesis such as CRISPR-Cas9. In this review, we describe mechanisms sense adverse conditions examine discuss classic new select improve crops.
Язык: Английский
Процитировано
48
Bioengineering, Год журнала: 2022, Номер 9(4), С. 176 - 176
Опубликована: Апрель 18, 2022
Food security and crop production are challenged worldwide due to overpopulation, changing environmental conditions, establishment failure, various kinds of post-harvest losses. The demand for high-quality foods with improved nutritional quality is also growing day by day. Therefore, produce reducing losses produce, particularly perishable fruits vegetables, vital. For many decades, attempts have been made improve the traits horticultural crops. Recently, modern genetic tools such as genome editing emerged a new approach manage overcome effectively efficiently. different including ZFNs, TALENs, CRISPR/Cas9 system introduce mutations (In Dels) in crops address resolve issues associated storage quality. Henceforth, we provide broad review applications horticulture stability shelf life, texture, resistance pathogens without compromising value. Moreover, major roadblocks, challenges, their possible solutions employing discussed.
Язык: Английский
Процитировано
43
GM crops & food, Год журнала: 2023, Номер 14(1), С. 1 - 20
Опубликована: Янв. 6, 2023
Domestication of orphan crops could be explored by editing their genomes. Genome has a lot promise for enhancing agricultural output, and there is interest in furthering breeding crops, which are sometimes plagued with unwanted traits that resemble wild cousins. Consequently, applying model crop knowledge to allows the rapid generation targeted allelic diversity innovative germplasm. We explain how plant breeders employ genome as novel platform accelerate domestication semi-domesticated or plants, resulting more diversified base future food fodder supplies. This review emphasizes both practicality strategy need invest research advances our understanding genomes, genes, cellular systems. Planting these abandoned help alleviate scarcities challenge climate crises.
Язык: Английский
Процитировано
34
Biology, Год журнала: 2023, Номер 12(7), С. 1037 - 1037
Опубликована: Июль 22, 2023
The revolutionary CRISPR/Cas9 genome-editing technology has emerged as a powerful tool for plant improvement, offering unprecedented precision and efficiency in making targeted gene modifications. This practical approach to genome editing offers tremendous opportunities crop surpassing the capabilities of conventional breeding techniques. article provides an overview recent advancements challenges associated with application improvement. potential terms developing crops enhanced resistance biotic abiotic stresses is highlighted, examples genes edited confer disease resistance, drought tolerance, salt cold tolerance. Here, we also discuss importance off-target effects efforts made mitigate them, including use shorter single-guide RNAs dual Cas9 nickases. Furthermore, alternative delivery methods, such protein- RNA-based approaches, are explored, they could potentially avoid integration foreign DNA into genome, thus alleviating concerns related genetically modified organisms (GMOs). We emphasize significance accelerating processes, reducing time costs, enabling introduction desired traits at nucleotide level. As field continues evolve, it anticipated that will remain prominent adaptation challenging environmental conditions.
Язык: Английский
Процитировано
32
Plant Cell Reports, Год журнала: 2024, Номер 43(4)
Опубликована: Март 18, 2024
Abstract Genome-editing technologies have revolutionized research in plant biology, with major implications for agriculture and worldwide food security, particularly the face of challenges such as climate change increasing human populations. Among these technologies, clustered regularly interspaced short palindromic repeats [CRISPR]–CRISPR-associated protein [Cas] systems are now widely used editing crop genomes. In this review, we provide an overview CRISPR–Cas technology its most significant applications improving sustainability. We also review current potential technological advances that will aid future breeding crops to enhance security worldwide. Finally, discuss obstacles must be overcome realize maximum genome-editing production.
Язык: Английский
Процитировано
11
Current Gene Therapy, Год журнала: 2024, Номер 24(3), С. 193 - 207
Опубликована: Фев. 4, 2024
With the discovery of CRISPR-Cas9, drug development and precision medicine have undergone a major change. This review article looks at new ways that CRISPR-based therapies are being used how they changing way is done. CRISPR technology's ability to precisely flexibly edit genes has opened up find, validate, develop targets. Also, it made for personalized gene therapies, precise editing, advanced screening techniques, all which hold great promise treating wide range diseases. In this article, we look latest research clinical trials show could be treat genetic diseases, cancer, infectious other hard-to-treat conditions. However, ethical issues problems with regulations also discussed in relation shows important use them safely responsibly. As continues change drugs used, shines light on amazing things been done what future might rapidly field.
Язык: Английский
Процитировано
10
Frontiers in Plant Science, Год журнала: 2021, Номер 12
Опубликована: Авг. 10, 2021
Crop production has been substantially reduced by devastating fungal and oomycete pathogens, these pathogens continue to threaten global food security. Although chemical cultural controls have used for crop protection, involve continuous costs time fungicide resistance among plant increasingly reported. The most efficient way protect crops from is cultivation of disease-resistant cultivars. However, traditional breeding approaches are laborious intensive. Recently, the CRISPR/Cas9 system utilized enhance disease different such as rice, cacao, wheat, tomato, grape. This allows precise genome editing various organisms via RNA-guided DNA endonuclease activity. Beyond in crops, genomes can also provide new strategies management. review focuses on recent studies against using system. For long-term management, targeting multiple mechanisms with insights gained probing this will be powerful approaches.
Язык: Английский
Процитировано
41