Abstract
Introduction
Optimal
exploitation
of
the
huge
amounts
agro-industrial
residuals
that
are
produced
annually,
which
endangers
ecosystem
and
ultimately
contributes
to
climate
change,
is
one
solutions
available
produce
value-added
compounds.
Aim
objectives
This
study
aimed
at
economic
production
optimization
surfactin.
Therefore,
was
carried
out
by
microbial
conversion
Potato
Peel
Waste
(PPW)
Frying
Oil
(FOW)
utilizing
locally
isolated
Bacillus
halotolerans.
Also,
investigating
its
potential
application
as
an
antimicrobial
agent
towards
some
pathogenic
strains.
Results
Screening
bacterial
isolates
for
surfactin
revealed
strain
with
highest
yield
(49
g/100
g
substrate)
efficient
oil
displacement
activity
genetically
identified
B.
halotolerans
.
The
process
then
optimized
Central
Composite
Design
(CCD)
resulting
in
amelioration
11.4%
(from
49
55.3
surface
tension
(ST)
8.3%
36
33
mN/m)
a
constant
level
critical
micelle
concentration
(CMC)
125
mg/L.
Moreover,
physiochemical
characterization
studies
FTIR,
1
H
NMR,
LC–MS/MS
proved
existence
cyclic
lipopeptide
(surfactin).
investigations
further
showed
strong
emulsification
affinity
soybean
motor
(E24
=
50%),
well
ability
maintain
emulsion
stable
over
wide
pH
(4–10)
temperature
(10–100
°C)
range.
Interestingly,
had
broad-spectrum
range
inhibition
against
subtilis,
Staphylococcus
aureus,
Pseudomonas
aeruginosa,
klebsiella
pneumonia
,
Candida
albicans.
Conclusion
Subsequently,
screening
utilized
food-processing
wastes
along
extraction
technique
resulted
high
characterized
acceptable
ST
CMC
levels.
However,
cultural
conditions
improve
productivity
achieved
using
Factor-At-A-Time
(OFAT)
(CCD).
In
contrast,
recorded
maximum
(33
mN/n)
substrate.
also
stability
emulsions
temperature.
Otherwise,
obtained
results
promising
efficiency
fungal
pathogens.
Current Research in Biotechnology,
Год журнала:
2024,
Номер
7, С. 100227 - 100227
Опубликована: Янв. 1, 2024
Nanoscale
copper
oxide
(CuO
NPs)
with
diameters
in
the
80–150
nm
range
has
been
biosynthesized
using
lipopeptide
biosurfactant
derived
from
Bacillus
vallismortis
and
characterized
by
XRD
FE-SEM.
The
CuO
NPs
could
be
introduced
as
nanocarrier
systems
for
combination
therapy
a
potential
candidate
antibacterial,
antioxidant,
anticancer,
anti-diabetic
activity.
antibacterial
activity
of
was
studied
incorporating
nanoparticles
fluorescent
antibiotic
Ciprofloxacin
HCL
(CIP)
to
form
CIP@CuO
tracked
inside
HEK-293
cell
lines.
MIC
values
against
1
×
106
CFU
ml−1
Pseudomonas
aeruginosa
determined
76
µg
ml−1and
69
Staphylococcus
aureus.
were
conjugated
anticancer
drug
Doxorubicin
(DOX)
DOX@CuONPs,
improving
delivery
toward
cancer
(HeLa)
cells.
intracellular
uptake
drug-loaded
confirmed
confocal
micrographs.
Finally,
vitro
lipopeptide-coated
inhibitory
α-amylase.
In
contrast,
vivo
efficacy
validated
significant
reduction
blood
glucose
glutathione
levels.
positively
affected
histopathological
changes
pancreas
induced
diabetic
mice.
Cytotoxicity
testing
Zebrafish
demonstrated
abnormal
organ
development
varying
viability
hatching
rates
at
72
96
hpf,
an
LC50
45
µg/l.
Aside
various
medicinal
characteristics,
study
provided
valuable
information
on
cytotoxic
impact,
which
can
used
future
investigations
their
eco-toxicological
impacts.
Abstract
Introduction
Optimal
exploitation
of
the
huge
amounts
agro-industrial
residuals
that
are
produced
annually,
which
endangers
ecosystem
and
ultimately
contributes
to
climate
change,
is
one
solutions
available
produce
value-added
compounds.
Aim
objectives
This
study
aimed
at
economic
production
optimization
surfactin.
Therefore,
was
carried
out
by
microbial
conversion
Potato
Peel
Waste
(PPW)
Frying
Oil
(FOW)
utilizing
locally
isolated
Bacillus
halotolerans.
Also,
investigating
its
potential
application
as
an
antimicrobial
agent
towards
some
pathogenic
strains.
Results
Screening
bacterial
isolates
for
surfactin
revealed
strain
with
highest
yield
(49
g/100
g
substrate)
efficient
oil
displacement
activity
genetically
identified
B.
halotolerans
.
The
process
then
optimized
Central
Composite
Design
(CCD)
resulting
in
amelioration
11.4%
(from
49
55.3
surface
tension
(ST)
8.3%
36
33
mN/m)
a
constant
level
critical
micelle
concentration
(CMC)
125
mg/L.
Moreover,
physiochemical
characterization
studies
FTIR,
1
H
NMR,
LC–MS/MS
proved
existence
cyclic
lipopeptide
(surfactin).
investigations
further
showed
strong
emulsification
affinity
soybean
motor
(E24
=
50%),
well
ability
maintain
emulsion
stable
over
wide
pH
(4–10)
temperature
(10–100
°C)
range.
Interestingly,
had
broad-spectrum
range
inhibition
against
subtilis,
Staphylococcus
aureus,
Pseudomonas
aeruginosa,
klebsiella
pneumonia
,
Candida
albicans.
Conclusion
Subsequently,
screening
utilized
food-processing
wastes
along
extraction
technique
resulted
high
characterized
acceptable
ST
CMC
levels.
However,
cultural
conditions
improve
productivity
achieved
using
Factor-At-A-Time
(OFAT)
(CCD).
In
contrast,
recorded
maximum
(33
mN/n)
substrate.
also
stability
emulsions
temperature.
Otherwise,
obtained
results
promising
efficiency
fungal
pathogens.
