Macrophage Membrane-Coated Nanoparticles for the Delivery of Natamycin Exhibit Increased Antifungal and Anti-Inflammatory Activities in Fungal Keratitis DOI
Xing Liu, Yunfeng Zhang, Fang Peng

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(44), С. 59777 - 59788

Опубликована: Окт. 28, 2024

This study aims to explore the efficacy and safety of macrophage membrane-coated nanoparticles for delivery natamycin (NAT) in therapy fungal keratitis (FK). Macrophage membranes were isolated identified by immunofluorescence staining (IFS). NAT was encapsulated into poly(lactic-co-glycolic acid) (PLGA). Fungal stimulated (M1) or unstimulated (M) separately mixed sonicated with PLGA nanoparticles. The biocompatible (PLGA-NAT, PLGA-NAT@M, PLGA-NAT@M1) characterized zeta-sizer analysis, transmission electron microscopy (TEM), Western blot. Drug encapsulation loading efficiency release detected ultraviolet spectrophotometry. cytotoxicity, ocular surface toxicity irritability, systemic different concentrations assessed. In vitro, we examined antifungal properties eye retention time, drug release, curative effects on FK evaluated vitro vivo. IFS results showed separation membrane nucleus. prepared had a typical "core–shell" structure uniform nanometer size, proteins retained allowing exert functional macrophage. efficiencies PLGA-NAT@M PLGA-NAT@M1 7.6 6.7%, respectively. 51.2 41.5%, could gradually reduce clearance surface. enhanced activity PLGA-NAT. Furthermore, coated increased biocompatibility decreased corneal vivo, significantly alleviated severity FK. PLGA@M PLGA@M1 reduced protein levels inflammatory cytokines after stimulation. has good physical biosafety. It evade clearance, gradually, achieve high anti-inflammatory clinically have application potential treatment

Язык: Английский

Biomimetic nanoparticles functionalized by macrophage membrane ameliorate heart failure in mouse and human cardiac organoid model DOI

Jianjun Gu,

Lina Zhang, Jia You

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 159447 - 159447

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Macrophage Membrane-Cloaked ROS-Responsive Albumin Nanoplatforms for Targeted Delivery of Curcumin to Alleviate Acute Liver Injury DOI

Dandan Hu,

Zhenqiu Huang, Wenlong Li

и другие.

Molecular Pharmaceutics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Developing low-toxicity, high-efficacy, and fast-acting strategies to manage acute liver injury (ALI) is critical due its rapid progression potential for severe outcomes. Curcumin (CUR) has shown promise in ALI therapy ability modulate the inflammatory microenvironment by scavenging reactive oxygen species (ROS). Nevertheless, CUR highly hydrophobic limiting bioavailability effective vivo transport, which hinders further application. In this study, we developed an microenvironment-targeted drug delivery system covalently coupling human serum albumin (HSA) with ROS-sensitive thioketal linkers loading it form nanoparticles (HSA-TK/CUR). These were then coated a macrophage membrane (CM@HSA-TK/CUR), resulting negatively charged spherical particles (≈ −23.26 mV) average particle size of around 165 nm. ROS responsiveness was confirmed through release assays enhanced depletion demonstrated Diacetyldichlorofluorescein (DCFH-DA) detection experiments. CM@HSA-TK/CUR treatment resulted 94.7% reduction levels cells. addition, cellular uptake distribution experiments that camouflaging HSA-TK/CUR membranes significantly targeting microenvironment. The findings revealed rapidly accumulated injured within 6 h, inhibited production pro-inflammatory factors (IL-1β, IL-6, TNF-α), shifted polarization from M1 M2 vivo, protected hepatocytes oxidative stress-associated cell death, attenuating response mice. conclusion, excellent treating mice ALI.

Язык: Английский

Процитировано

0

Ferroptosis as a key player in the pathogenesis and intervention therapy in liver injury: focusing on drug-induced hepatotoxicity DOI

Bahaa Ibrahim Saeed,

Subasini Uthirapathy,

Aziz Kubaev

и другие.

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Язык: Английский

Процитировано

0

Quercetin: A Flavonoid with Potential for Treating Acute Lung Injury DOI Creative Commons

M X Huang,

Xinxin Liu,

Yingcong Ren

и другие.

Drug Design Development and Therapy, Год журнала: 2024, Номер Volume 18, С. 5709 - 5728

Опубликована: Дек. 1, 2024

In intensive care units, acute lung injury (ALI) is a syndrome that frequently encountered. It associated with high rate of morbidity and mortality. Despite the extensive research conducted by medical community on its treatment, no specific effective drugs have been identified. Quercetin natural flavonoid many biological activities pharmacological effects. Research indicates can modulate various targets signaling pathways, inhibiting oxidative stress, inflammatory responses, ferroptosis, apoptosis, fibrosis, bacterial viral infections in ALI. This regulation suggests potential therapeutic application for condition. Currently, there comprehensive review addressing treatment paper begins classification ALI, followed detailed summary mechanisms through which may treat ALI to evaluate as novel option.

Язык: Английский

Процитировано

2

Macrophage Membrane-Coated Nanoparticles for the Delivery of Natamycin Exhibit Increased Antifungal and Anti-Inflammatory Activities in Fungal Keratitis DOI
Xing Liu, Yunfeng Zhang, Fang Peng

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(44), С. 59777 - 59788

Опубликована: Окт. 28, 2024

This study aims to explore the efficacy and safety of macrophage membrane-coated nanoparticles for delivery natamycin (NAT) in therapy fungal keratitis (FK). Macrophage membranes were isolated identified by immunofluorescence staining (IFS). NAT was encapsulated into poly(lactic-co-glycolic acid) (PLGA). Fungal stimulated (M1) or unstimulated (M) separately mixed sonicated with PLGA nanoparticles. The biocompatible (PLGA-NAT, PLGA-NAT@M, PLGA-NAT@M1) characterized zeta-sizer analysis, transmission electron microscopy (TEM), Western blot. Drug encapsulation loading efficiency release detected ultraviolet spectrophotometry. cytotoxicity, ocular surface toxicity irritability, systemic different concentrations assessed. In vitro, we examined antifungal properties eye retention time, drug release, curative effects on FK evaluated vitro vivo. IFS results showed separation membrane nucleus. prepared had a typical "core–shell" structure uniform nanometer size, proteins retained allowing exert functional macrophage. efficiencies PLGA-NAT@M PLGA-NAT@M1 7.6 6.7%, respectively. 51.2 41.5%, could gradually reduce clearance surface. enhanced activity PLGA-NAT. Furthermore, coated increased biocompatibility decreased corneal vivo, significantly alleviated severity FK. PLGA@M PLGA@M1 reduced protein levels inflammatory cytokines after stimulation. has good physical biosafety. It evade clearance, gradually, achieve high anti-inflammatory clinically have application potential treatment

Язык: Английский

Процитировано

2