The induction of metallothionein by sulforaphane reduces iron toxicity via Nrf2 DOI Open Access

Amanda Putri Elvandari,

Ferbian Milas Siswanto, Susumu Imaoka

и другие.

Journal of Applied Biology & Biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Iron is essential for cell survival; however, iron overload disorders lead to excess deposition, which can induce oxidative stress and reduce viability. Rapid chelation of prevent critical avoiding the negative impacts overload. Iron-chelating agents are often used treat overload; most these have adverse side effects a narrow scope applications. Recently, many classes phytochelators with additional antioxidant activities been developed clinical use. Sulforaphane (SFN) has potent cytoprotective activities, but its role in metabolism never reported. Thus, we assessed protective SFN against iron-induced toxicity hepatoma Hep3B cells examined mechanism. In this study, Fe ions increased intracellular ROS levels decreased An transcription factor called NF-E2-related 2 (Nrf2) was activated by treatment. chelator deferoxamine (DFO) reduced did not affect Next, showed that improved viability further enhancing Nrf2 via phosphoinositide 3-kinase (PI3K) pathway. Mechanistically, transcriptionally metallothionein (MT), metal-binding protein. Overexpression MT protected from toxicity, similar activities. These results suggest induction mRNA mechanisms contributing disorders.

Язык: Английский

Applications of hydrogels and nanoparticles in the treatment of traumatic brain injury DOI Creative Commons

Jiaying Shi,

Jiajia Tang, Xu Jin

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 12

Опубликована: Янв. 6, 2025

Traumatic brain injury (TBI) represents a significant global public health issue, with effective management posing numerous challenges. The pathophysiology of TBI is typically categorized into two phases: primary and secondary injuries. Secondary involves pathophysiological mechanisms such as blood-brain barrier (BBB) disruption, mitochondrial dysfunction, oxidative stress, inflammatory responses. Current pharmacological strategies often encounter obstacles in treating effectively, primarily due to challenges BBB penetration, inadequate target site accumulation, off-target toxicity. Versatile hydrogels nanoparticles offer potential solutions these limitations. This review discusses recent progress utilizing for treatment over the past 5 years, highlighting their relevance underlying pathophysiology. Hydrogels demonstrate substantial promise addressing injury, providing broad spectrum future therapeutic opportunities.

Язык: Английский

Процитировано

3

Application of stimuli-responsive hydrogel in brain disease treatment DOI Creative Commons

Bingqing Xie,

Huangfan Xie

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Июль 18, 2024

Treating brain diseases presents significant challenges due to neuronal degeneration, inflammation, and the intricate nature of brain. Stimuli-responsive hydrogels, designed closely resemble brain’s extracellular matrix, have emerged as promising candidates for controlled drug delivery tissue engineering. These hydrogels unique ability encapsulate therapeutic agents release them in a manner when triggered by environmental stimuli. This property makes particularly suitable delivering drugs precisely targeted areas brain, while minimizing collateral damage healthy tissue. Their preclinical success treating various animal studies underscores their translational potential human disease treatment. However, deeper understanding long-term behavior, biodistribution, biocompatibility within remains crucial. Furthermore, exploring novel hydrogel systems combinations is paramount advancing towards more effective treatments. review summarizes latest advancements this field over past 5 years, specifically highlighting progress with stimuli-responsive diseases.

Язык: Английский

Процитировано

5

Hydrogel in the treatment of traumatic brain injury DOI Creative Commons
Shanhe Li, Jiajun Xu,

Yuqing Qian

и другие.

Biomaterials Research, Год журнала: 2024, Номер 28

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

4

The induction of metallothionein by sulforaphane reduces iron toxicity via Nrf2 DOI Open Access

Amanda Putri Elvandari,

Ferbian Milas Siswanto, Susumu Imaoka

и другие.

Journal of Applied Biology & Biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Iron is essential for cell survival; however, iron overload disorders lead to excess deposition, which can induce oxidative stress and reduce viability. Rapid chelation of prevent critical avoiding the negative impacts overload. Iron-chelating agents are often used treat overload; most these have adverse side effects a narrow scope applications. Recently, many classes phytochelators with additional antioxidant activities been developed clinical use. Sulforaphane (SFN) has potent cytoprotective activities, but its role in metabolism never reported. Thus, we assessed protective SFN against iron-induced toxicity hepatoma Hep3B cells examined mechanism. In this study, Fe ions increased intracellular ROS levels decreased An transcription factor called NF-E2-related 2 (Nrf2) was activated by treatment. chelator deferoxamine (DFO) reduced did not affect Next, showed that improved viability further enhancing Nrf2 via phosphoinositide 3-kinase (PI3K) pathway. Mechanistically, transcriptionally metallothionein (MT), metal-binding protein. Overexpression MT protected from toxicity, similar activities. These results suggest induction mRNA mechanisms contributing disorders.

Язык: Английский

Процитировано

0