Rapid Volumetric Bioprinting of Decellularized Extracellular Matrix Bioinks

Advanced Materials, Год журнала: 2024, Номер 36(34)

Опубликована: Янв. 22, 2024

Decellularized extracellular matrix (dECM)-based hydrogels are widely applied to additive biomanufacturing strategies for relevant applications. The components and growth factors of dECM play crucial roles in cell adhesion, growth, differentiation. However, the generally poor mechanical properties printability have remained as major limitations dECM-based materials. In this study, heart-derived (h-dECM) meniscus-derived (Ms-dECM) bioinks their pristine, unmodified state supplemented with photoinitiator system tris(2,2-bipyridyl) dichlororuthenium(II) hexahydrate sodium persulfate, demonstrate cytocompatibility volumetric bioprinting processes. This recently developed modality illuminates a dynamically evolving light pattern into rotating volume bioink, thus decouples requirement strengths bioprinted hydrogel constructs printability, allowing fabrication sophisticated shapes architectures low-concentration materials that set within tens seconds. As exemplary applications, cardiac tissues volumetrically using cardiomyocyte-laden h-dECM bioink showing favorable proliferation, expansion, spreading, biomarker expressions, synchronized contractions; whereas Ms-dECM meniscus structures embedded human mesenchymal stem cells present appropriate chondrogenic differentiation outcomes. study supplies expanded libraries broadens utilities toward tissue engineering regenerative medicine.

Язык: Английский

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4D bioprinting of programmed dynamic tissues

Bioactive Materials, Год журнала: 2024, Номер 37, С. 348 - 377

Опубликована: Апрель 23, 2024

Setting time as the fourth dimension, 4D printing allows us to construct dynamic structures that can change their shape, property, or functionality over under stimuli, leading a wave of innovations in various fields. Recently, smart biomaterials, biological components, and living cells into 3D constructs with effects has led an exciting field bioprinting. bioprinting gained increasing attention is being applied create programmed cell-laden such bone, cartilage, vasculature. This review presents overview on for engineering tissues organs, followed by discussion approaches, technologies, biomaterials design, bioink requirements, applications. While much progress been achieved, complex process facing challenges need be addressed transdisciplinary strategies unleash full potential this advanced biofabrication technology. Finally, we present future perspectives rapidly evolving bioprinting, view its potential, increasingly important roles development basic research, pharmaceutics, regenerative medicine.

Язык: Английский

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Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Chemical Reviews, Год журнала: 2024, Номер 124(14), С. 8787 - 8822

Опубликована: Июль 5, 2024

Harnessing light for cross-linking of photoresponsive materials has revolutionized the field 3D printing. A wide variety techniques leveraging broad-spectrum shaping have been introduced as a way to achieve fast and high-resolution printing, with applications ranging from simple prototypes biomimetic engineered tissues regenerative medicine. Conventional light-based printing use material in layer-by-layer fashion produce complex parts. Only recently, new emerged which deploy multidirection, tomographic, light-sheet or filamented image projections deep into volume resin-filled vat photoinitiation cross-linking. These Deep Vat (DVP) approaches alleviate need layer-wise enable unprecedented fabrication speeds (within few seconds) high resolution (>10 μm). Here, we elucidate physics chemistry these processes, their commonalities differences, well emerging biomedical non-biomedical fields. Importantly, highlight limitations, future scope research that will improve scalability applicability DVP engineering medicine applications.

Язык: Английский

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Volumetric Additive Manufacturing for Cell Printing: Bridging Industry Adaptation and Regulatory Frontiers

ACS Biomaterials Science & Engineering, Год журнала: 2025, Номер 11(1), С. 156 - 181

Опубликована: Янв. 2, 2025

Volumetric additive manufacturing (VAM) is revolutionizing the field of cell printing by enabling rapid creation complex three-dimensional cellular structures that mimic natural tissues. This paper explores advantages and limitations various VAM techniques, such as holographic lithography, digital light processing, volumetric projection, while addressing their suitability across diverse industrial applications. Despite significant potential VAM, challenges related to regulatory compliance scalability persist, particularly in context bioprinted In India, lack clear guidelines intellectual property protections poses additional hurdles for companies seeking navigate evolving landscape bioprinting. study emphasizes importance collaboration among industry stakeholders, agencies, academic institutions establish tailored frameworks promote innovation ensuring safety efficacy. By bridging gap between technological advancement oversight, can unlock new opportunities regenerative medicine tissue engineering, transforming patient care therapeutic outcomes.

Язык: Английский

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Computational Fluid Dynamics (CFD) Analysis of Bioprinting

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(20)

Опубликована: Апрель 22, 2024

Regenerative medicine has evolved with the rise of tissue engineering due to advancements in healthcare and technology. In recent years, bioprinting been an upcoming approach traditional practices, through fabrication functional by its layer-by-layer deposition process. This overcomes challenges such as irregular cell distribution limited density, it can potentially address organ shortages, increasing transplant options. Bioprinting fully organs is a long stretch but advancement rapidly growing precision compatibility complex geometries. Computational Fluid Dynamics (CFD), carestone computer-aided engineering, instrumental assisting research development cutting costs saving time. CFD optimizes testing parameters shear stress, diffusivity, viability, reducing repetitive experiments aiding material selection bioprinter nozzle design. review discusses current application potential enhance technology that contribute evolution regenerative medicine.

Язык: Английский

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Bioinks and biofabrication techniques for biosensors development: A review

Materials Today Bio, Год журнала: 2024, Номер 28, С. 101185 - 101185

Опубликована: Авг. 5, 2024

3D bioprinting technologies and bioink development are enabling significant advances in miniaturized integrated biosensors. For example, bioreceptors can be immobilized within a porous structure to significantly amplify the signal, while biocompatible mechanically flexible systems uniquely enable wearable chem- bio-sensors. This advancement is accelerating translation by production of high performance, reproducible, analytical devices. The formulation plays crucial role determining bio-functionality resulting printed structures, e.g., porosity that allows analyte diffuse through structure, affinity avidity receptors, etc. review explores next generation advanced bioinks for biosensor provides insights into latest cutting-edge technologies. methods available fabrication including inkjet, extrusion, laser-based bioprinting, discussed. advantages limitations each method analysed, recent advancements presented. then delves properties bioinks, such as biocompatibility, printability, stability, applicability. Different types explored, multicomponent, stimuli-responsive, conductive bioinks. Finally, biosensors considered, identifying possible new opportunities challenges. Overall, this literature highlights combined importance high-performance

Язык: Английский

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Advanced material technologies for space and terrestrial medicine

Nature Reviews Materials, Год журнала: 2024, Номер unknown

Опубликована: Июнь 3, 2024

Язык: Английский

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Recent advances in 3D bioprinting of tissues and organs for transplantation and drug screening

Virtual and Physical Prototyping, Год журнала: 2024, Номер 19(1)

Опубликована: Авг. 5, 2024

Organ transplantation is the optimal treatment for patients with end-stage organ failure, but which faces challenge of donor shortage. Two-dimensional cell culture and animal experiments are difficult to completely simulate complex cellular microenvironment drug testing. Three-dimensional (3D) bioprinting an emerging manufacturing technology fabricate artificial tissues organs screening. This review first describes technologies used constructs, including jetting-based, extrusion-based, vat photopolymerization-based methods other 3D approaches. The various kinds bioinks, sources, most recent applications in testing subsequently summarized. Finally, we discuss challenges prospects organs. aims facilitate overcoming obstacles identified on challenging journey towards adoption tissue

Язык: Английский

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The utilisation of biliary organoids for biomedical applications

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 12

Опубликована: Янв. 7, 2025

Biliary duct injury, biliary atresia (BA), tract tumors, primary sclerosing cholangitis (PSC), and other diseases are commonly encountered in clinical practice within the digestive system. To gain a better understanding of pathogenesis development these explore more effective treatment methods, organoid technology has recently garnered significant attention. Organoids three-dimensional structures derived from stem/progenitor cells that can faithfully mimic intricate structure physiological function tissues or organs vitro. They provide valuable platform for studying offer novel possibilities repairing regenerating injuries. The main seed used to construct organoids include human epithelial as well pluripotent stem cells. construction involves various techniques such traditional embedding technology, rotary culture hanging drop along with emerging approaches like organ chip (3D) printing four-dimensional (4D) technology. This article comprehensively reviews methods while discussing their applications disease modeling research on mechanisms drug screening tissue/organ repair; it also highlights current challenges suggests future directions regarding which will serve references treating common refractory system practice.

Язык: Английский

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A review of 3D bioprinting for organoids

Medical Review, Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

Abstract Current two-dimensional (2D) cell models for effective drug screening suffer from significant limitations imposed by the lack of realism in physiological environment. Three-dimensional (3D) organoids hold immense potential mimicking key functions human organs overcoming traditional 2D models. However, current techniques preparation 3D had reproducibility, scalability, and ability to closely replicate complex microenvironment found vivo . Additionally, culture systems often involve lengthy labor-intensive processes that hinder high-throughput applications necessary a large-scale screening. Advancements bioprinting technologies offer promising solutions these challenges enabling precise spatial control over placement material composition, thereby facilitating creation more physiologically relevant than techniques. This review provides comprehensive summary recent advances creating models, which begins with an introduction different types (especially focus on volumetric (VBP) technique), followed overview bioinks utilized bioprinting. Moreover, we also introduce disease efficiency evaluation regenerative medicine. Finally, possible strategies development clinical translation are concluded.

Язык: Английский

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