iScience, Год журнала: 2024, Номер 28(1), С. 111664 - 111664
Опубликована: Дек. 20, 2024
Язык: Английский
Bioengineering, Год журнала: 2025, Номер 12(2), С. 136 - 136
Опубликована: Янв. 30, 2025
Whole-organ decellularization generates scaffolds containing native extracellular matrix (ECM) components with preserved tissue microarchitecture, providing a promising advancement in engineering and regenerative medicine. Decellularization retains the ECM integrity which is important for supporting cell attachment, growth, differentiation, biological function. Although there are consensus guidelines to standardize processes characterization, no specific criteria or standards regarding sterility biosafety have been established so far. This regulatory gap safety, sterilization, regulation has hampered clinical translation of decellularized scaffolds. In this review, we identify essential safe use products from both human animal sources. These include efficacy, levels chemical residue, preservation composition physical characteristics, aseptic processing assure sterility. Furthermore, explore key considerations advancing into practice, focusing on frameworks safety requirements. Addressing these challenges crucial minimizing risks adverse reactions infection transmission, thereby accelerating adoption tissue-engineered products. review aims provide foundation establishing robust guidelines, effective integration medicine applications.
Язык: Английский
Процитировано
2
Chemical Reviews, Год журнала: 2024, Номер 124(14), С. 8787 - 8822
Опубликована: Июль 5, 2024
Harnessing light for cross-linking of photoresponsive materials has revolutionized the field 3D printing. A wide variety techniques leveraging broad-spectrum shaping have been introduced as a way to achieve fast and high-resolution printing, with applications ranging from simple prototypes biomimetic engineered tissues regenerative medicine. Conventional light-based printing use material in layer-by-layer fashion produce complex parts. Only recently, new emerged which deploy multidirection, tomographic, light-sheet or filamented image projections deep into volume resin-filled vat photoinitiation cross-linking. These Deep Vat (DVP) approaches alleviate need layer-wise enable unprecedented fabrication speeds (within few seconds) high resolution (>10 μm). Here, we elucidate physics chemistry these processes, their commonalities differences, well emerging biomedical non-biomedical fields. Importantly, highlight limitations, future scope research that will improve scalability applicability DVP engineering medicine applications.
Язык: Английский
Процитировано
10
Engineering, Год журнала: 2024, Номер unknown
Опубликована: Июнь 1, 2024
Organ damage or failure arising from injury, disease, and aging poses challenges due to the body's limited regenerative capabilities. transplantation presents issues of donor shortages immune rejection risks, necessitating innovative solutions. The 3D bioprinting organs on demand offers promise in tissue engineering medicine. In this review, we explore state-of-the-art technologies, with a focus bioink cell type selections. We follow discussions advances solid organs, such as heart, liver, kidney, pancreas, highlighting importance vascularization integration. Finally, provide insights into key future directions context clinical translation bioprinted their large-scale production.
Язык: Английский
Процитировано
8
Chemical Engineering Journal, Год журнала: 2024, Номер 497, С. 154627 - 154627
Опубликована: Авг. 7, 2024
Язык: Английский
Процитировано
8
Aggregate, Год журнала: 2025, Номер unknown
Опубликована: Янв. 9, 2025
ABSTRACT The repair and functional reconstruction of bone defects resulting from trauma, surgical resection, degenerative diseases, congenital malformations are major clinical challenges. Bone tissue engineering has significant advantages in the treatment severe defects. Vascularized scaffolds gradually attracting attention development because their excellent biomimetic properties efficient efficiency. Three‐dimensional (3D) printing technology, which can be used to fabricate structures at different scales using a wide range materials, been production vascularized scaffolds. This review discusses research progress 3D for Angiogenesis‐osteogenesis coupling regeneration process is first introduced, followed by summary technologies, inks, bioactive factors Notably, this focuses on structural design strategies Finally, application medicine, as well challenges outlooks future development, described.
Язык: Английский
Процитировано
1
iScience, Год журнала: 2025, Номер 28(3), С. 111882 - 111882
Опубликована: Янв. 23, 2025
Cardiac tissue lacks regenerative capacity, making heart transplantation the primary treatment for end-stage failure. Engineered cardiac tissues developed through three-dimensional bioprinting (3DBP) offer a promising alternative. However, reproducing native structure, cellular diversity, and functionality of requires advanced bioinks. Major obstacles in CTE (cardiac engineering) include accurately characterizing bioink properties, replicating microenvironment, achieving precise spatial organization. Optimizing properties to closely mimic extracellular matrix (ECM) is essential, as deviations may result pathological effects. This review encompasses rheological electromechanical bioinks function microenvironment design functional constructs. Furthermore, it focuses on improving characteristics, printability, bioinks, offering valuable perspectives developing new especially designed CTE.
Язык: Английский
Процитировано
1
Advanced Materials, Год журнала: 2025, Номер unknown
Опубликована: Фев. 5, 2025
Engineered living systems (ELSs) represent purpose-driven assemblies of components, encompassing cells, biomaterials, and active agents, intricately designed to fulfill diverse biomedical applications. Gelatin its derivatives have been used extensively in ELSs owing their mature translational pathways, favorable biological properties, adjustable physicochemical characteristics. This review explores the intersection gelatin with fabrication techniques, offering a comprehensive examination synergistic potential creating for various applications biomedicine. It offers deep dive into gelatin, including structures production, sources, processing, properties. Additionally, techniques employing derivatives, generic microfluidics, 3D printing methods. Furthermore, it discusses based on regenerative engineering as well cell therapies, bioadhesives, biorobots, biosensors. Future directions challenges are also examined, highlighting emerging trends areas improvements innovations. In summary, this underscores significance gelatin-based advancing lays groundwork guiding future research developments within field.
Язык: Английский
Процитировано
1
Advanced Healthcare Materials, Год журнала: 2024, Номер 13(20)
Опубликована: Апрель 22, 2024
Regenerative medicine has evolved with the rise of tissue engineering due to advancements in healthcare and technology. In recent years, bioprinting been an upcoming approach traditional practices, through fabrication functional by its layer-by-layer deposition process. This overcomes challenges such as irregular cell distribution limited density, it can potentially address organ shortages, increasing transplant options. Bioprinting fully organs is a long stretch but advancement rapidly growing precision compatibility complex geometries. Computational Fluid Dynamics (CFD), carestone computer-aided engineering, instrumental assisting research development cutting costs saving time. CFD optimizes testing parameters shear stress, diffusivity, viability, reducing repetitive experiments aiding material selection bioprinter nozzle design. review discusses current application potential enhance technology that contribute evolution regenerative medicine.
Язык: Английский
Процитировано
7
Nature Reviews Bioengineering, Год журнала: 2024, Номер unknown
Опубликована: Окт. 16, 2024
Язык: Английский
Процитировано
7
Advanced Functional Materials, Год журнала: 2024, Номер unknown
Опубликована: Авг. 5, 2024
Abstract Reproductive health concerns like Mayer‐Rokitansky‐Küster‐Hauser (MRKH) syndrome are prevalent in today's society. MRKH is a condition that severely affects women's sexual life, fertility, and mental has high prevalence of one out 5000 female births. Vaginoplasty the primary method to regain patients’ reproductive health. However, conventional vaginoplasty faces various challenges, including complex non‐customized treatment procedures causing intense pains complications. To bring new advances vaginoplasty, 3D‐printed hydrogel scaffold developed provide satisfactory mechanical support bioactivity for accelerating defect repair after surgery. The consisting gelatin methacryloyl (gelMA) carrageenan (Car) custom using an ambient temperature printing system. Furthermore, undergoes dual‐crosslinking through chemical crosslinking gelMA ionic Car with magnesium ions (Mg 2+ ). This strategy substantially improves overall properties introduces bioactive Mg . sustained release plus extracts from dual‐crosslinked significantly promotes cell proliferation, migration angiogenesis. In preclinical rat model penetrating genital tract defects mimicking implantation repairs wounds near‐normal levels within week, showing potential as alternative better regaining
Язык: Английский
Процитировано
5