Bioprinting of self-healing materials and nanostructures for biomedical applications: Recent advances and progresses on fabrication and characterization techniques
Bioprinting,
Год журнала:
2024,
Номер
38, С. e00335 - e00335
Опубликована: Фев. 1, 2024
Язык: Английский
Micelle-Facilitated Gelation Kinetics and Viscoelasticity of Dynamic Hyaluronan Hydrogels for Bioprinting of Mimetic Constructs and Tissue Repair
Composites Part B Engineering,
Год журнала:
2025,
Номер
unknown, С. 112151 - 112151
Опубликована: Янв. 1, 2025
Язык: Английский
Bioprinted biomimetic hydrogel matrices guiding stem cell aggregates for enhanced chondrogenesis and cartilage regeneration
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
12(22), С. 5360 - 5376
Опубликована: Янв. 1, 2024
Articular
cartilage
tissue
has
limited
self-repair
capabilities,
with
damage
frequently
progressing
to
irreversible
degeneration.
Engineered
tissues
constructed
through
bioprinting
and
embedded
stem
cell
aggregates
offer
promising
therapeutic
alternatives.
Aggregates
of
bone
marrow
mesenchymal
stromal
cells
(BMSCs)
demonstrate
enhanced
more
rapid
chondrogenic
differentiation
than
isolated
cells,
thus
facilitating
repair.
However,
it
remains
a
key
challenge
precisely
control
biochemical
microenvironments
regulate
cellular
adhesion
cohesion
within
bioprinted
matrices
simultaneously.
Herein,
this
work
reports
bioprintable
hydrogel
matrix
high
aggregation
properties
for
The
comprises
an
cell-adhesive
gelatin
methacrylate
cell-cohesive
chitosan
(CHMA),
both
which
are
subjected
photo-initiated
crosslinking.
By
adjusting
the
CHMA
content,
mechanical
stability
cues
hydrogels
finely
tuned
promote
aggregation,
repair
implantation.
Multi-layer
constructs
encapsulated
BMSCs,
viability
reaching
91.1%,
photo-crosslinked
support
21
days.
BMSCs
rapidly
form
display
efficient
on
constructs,
as
evidenced
by
upregulated
expression
Sox9,
Aggrecan
Collagen
2a1
genes,
along
protein
levels.
Transplantation
these
BMSC-laden
into
cartilaginous
defects
demonstrates
effective
hyaline
Overall,
cell-responsive
scaffold
holds
immense
promise
applications
in
engineering.
Язык: Английский
Versatile xanthan gum-based support bath material compatible with multiple crosslinking mechanisms: rheological properties, printability, and cytocompatibility study
Biofabrication,
Год журнала:
2024,
Номер
16(3), С. 035005 - 035005
Опубликована: Апрель 2, 2024
Abstract
Extrusion-based
bioprinting
is
a
promising
technology
for
the
fabrication
of
complex
three-dimensional
(3D)
tissue-engineered
constructs.
To
further
improve
printing
accuracy
and
provide
mechanical
support
during
process,
hydrogel-based
bath
materials
have
been
developed.
However,
gel
structure
some
can
be
compromised
when
exposed
to
certain
bioink
crosslinking
cues,
hence
their
compatibility
with
bioinks
limited.
In
this
study,
xanthan
gum-based
composite
material
compatible
multiple
mechanisms
Different
different
underlying
polymeric
structures,
example,
particulate
suspensions
polymer
solution
varying
supramolecular
structure)
these
properties
are
governed
by
variety
intermolecular
interactions.
common
rheological
behavior
expected
because
they
similar
demonstrated
performance
functionality.
detailed
exploration/identification
expressed
from
unified
perspective,
benchmark
previous
studies
were
prepared.
A
comparative
study
revealed
both
structural
shear
characteristics
shared
materials,
including
yield
stress,
moduli,
shear-thinning
behavior,
self-healing
properties.
Gel
stability
functionality
tested
in
presence
various
stimuli,
confirming
versatility
xanthan-based
material.
We
investigated
effect
diameter
extrusion
needles
on
printability
demonstrate
improvement
integrity.
Cytotoxicity
cell
encapsulation
viability
tests
carried
out
confirm
propose
novel
new
insight
into
essential
that
serve
as
guide
development
bath-based
3D
bioprinting.
Язык: Английский
Magnetically Bioprinted Anisotropic Hydrogels Promote Bmsc Osteogenic Differentiation for Bone Defect Repair
Опубликована: Янв. 1, 2025
Язык: Английский
Fabrication of 3D Biomimetic Smooth Muscle Using Magnetic Induction and Bioprinting for Tissue Regeneration
Biomaterials Research,
Год журнала:
2024,
Номер
28
Опубликована: Янв. 1, 2024
Smooth
muscles
play
a
vital
role
in
peristalsis,
tissue
constriction,
and
relaxation
but
lack
adequate
self-repair
capability
for
addressing
extensive
muscle
defects.
Engineering
scaffolds
have
been
broadly
proposed
to
repair
the
tissue.
However,
efforts
date
shown
that
those
engineered
focus
on
cell
alignment
2-dimension
(2D)
fail
direct
cells
align
3D
area,
which
is
irresolvable
remodel
architecture
restore
functions
like
contraction
relaxation.
Herein,
we
introduced
an
iron
oxide
(Fe
3
O
4
)
filament-embedded
gelatin
(Gel)-silk
fibroin
composite
hydrogel
oriented
Fe
self-assembled
functioned
as
micro/nanoscale
geometric
cues
induce
growth.
The
scaffold
can
be
designed
fabricate
aligned
or
anisotropic
by
combining
embedded
bioprinting
with
magnetic
induction
accommodate
special
architectures
of
muscular
tissues
body.
Particularly,
bioprinted
muscle-like
matrices
effectively
promote
self-organization
smooth
(SMCs)
directional
differentiation
bone
marrow
mesenchymal
stem
(BMSCs)
into
SMCs.
This
biomimetic
accelerated
regeneration,
enhancing
intercellular
connectivity
within
tissue,
deposition
fibronectin
collagen
I.
work
provides
novel
approach
constructing
muscles,
holding
significant
promise
clinical
treatment
muscle-related
diseases
future.
Язык: Английский
Three-dimensional bioprinted cell-adaptive hydrogel with anisotropic micropores for enhancing skin wound healing
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
unknown, С. 136106 - 136106
Опубликована: Сен. 1, 2024
Язык: Английский
Gel-Based Suspension Medium Used in 3D Bioprinting for Constructing Tissue/Organ Analogs
Gels,
Год журнала:
2024,
Номер
10(10), С. 644 - 644
Опубликована: Окт. 10, 2024
Constructing
tissue/organ
analogs
with
natural
structures
and
cell
types
in
vitro
offers
a
valuable
strategy
for
the
situ
repair
of
damaged
tissues/organs.
Three-dimensional
(3D)
bioprinting
is
flexible
method
fabricating
these
analogs.
However,
extrusion-based
3D
faces
challenge
balancing
use
soft
bioinks
need
high-fidelity
geometric
shapes.
To
address
challenges,
recent
advancements
have
introduced
various
suspension
mediums
based
on
gelatin,
agarose,
gellan
gum
microgels.
The
emergence
gel-based
has
significantly
advanced
fabrication
constructs
using
bioprinting.
They
effectively
stabilize
support
bioinks,
enabling
formation
complex
spatial
geometries.
Moreover,
they
provide
stable,
cell-friendly
environment
that
maximizes
viability
during
printing
process.
This
minireview
will
summarize
properties,
preparation
methods,
potential
applications
constructing
analogs,
while
also
addressing
current
challenges
providing
an
outlook
future
Язык: Английский