MicroRNA-520a Suppresses Pathogenesis and Progression of Non-Small-Cell Lung Cancer through Targeting the RRM2/Wnt Axis

Analytical Cellular Pathology, Год журнала: 2021, Номер 2021, С. 1 - 12

Опубликована: Март 30, 2021

MicroRNAs (miRNAs) regulate multiple cellular behaviors, and their aberrant expression is frequently associated with disease progression. This research focused on the effects of miR-520a development non-small-cell lung cancer (NSCLC) molecules involved. Tumor normal tissues from 24 patients NSCLC were collected. Differentially expressed miRNAs between tumor screened using microarrays, was to be significantly poorly in samples. Artificial upregulation reduced proliferation, migration invasion, resistance death A549 H460 cells according MTT, EdU labeling, transwell, flow cytometry assays, respectively. suppressed growth metastasis xenograft tumors vivo. The integrated bioinformatic analysis dual luciferase assays suggested that targeted ribonucleotide reductase subunit 2 (RRM2) mRNA inactivated Wnt/β-catenin signaling pathway cells. Upregulation RRM2 enhanced malignant behaviors NSCLCs, but oncogenic blocked upon overexpression. To conclude, this study evidenced inhibits progression through suppressing Wnt pathway. paper may offer novel insights into treatment.

Язык: Английский

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Applications of quantum computing in clinical care

Frontiers in Medicine, Год журнала: 2025, Номер 12

Опубликована: Апрель 23, 2025

This review examines quantum computing (QC) applications in clinical care, emphasizing advancements directly impacting patient outcomes. QC holds transformative potential medicine, particularly through enhancing diagnostic accuracy, optimizing treatment plans, and enabling real-time decision-making. A systematic analysis of 35 studies published between 2015 2024 was conducted. The were evaluated for their contributions to diagnostic, therapeutic, decision-support improvements care enabled by technologies. revealed QC's promise improving accuracy medical imaging, treatments oncology, QC-driven algorithms demonstrated enhance computational efficiency. These could enable earlier detection diseases such as Alzheimer's, cancer, osteoarthritis, supporting more timely interventions better prognoses. Despite promising outcomes, current limitations-such hardware scalability, error mitigation, ethical considerations-hinder widespread adoption settings. Overcoming these challenges will require interdisciplinary collaboration technological innovation. underscores capacity deliver precise, personalized, efficient advocating its integration into healthcare workflows advance precision medicine improve

Язык: Английский

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CircDHDDS/miR-361-3p/WNT3A Axis Promotes the Development of Retinoblastoma by Regulating Proliferation, Cell Cycle, Migration, and Invasion of Retinoblastoma Cells

Neurochemical Research, Год журнала: 2020, Номер 45(11), С. 2691 - 2702

Опубликована: Авг. 31, 2020

Язык: Английский

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Shuttling of cellular proteins between the plasma membrane and nucleus (Review)

Molecular Medicine Reports, Год журнала: 2021, Номер 25(1)

Опубликована: Ноя. 10, 2021

Recently accumulated evidence has indicated that the nucleomembrane shuttling of cellular proteins is common, which provides new insight into subcellular translocation and biological functions synthesized in cytoplasm. The present study aimed to clarify trafficking between plasma membrane nucleus. These primarily consist transmembrane receptors, adaptor proteins, adhesive signal nuclear contribute proliferation, apoptosis, chemoresistance, adhesion, migration gene expression. frequently undergo cross‑talk, such as interaction with proteins. endocytosis, infusion organelles or proteolysis soluble forms for import nucleus, while interact act receptors. nucleocytosolic involves export through pores by importin exportin. Nuclear generally other transcription factors, then binding promoter expression, are responsible initiation and/or Protein occurs a cell‑specific manner closely linked events. review improve understanding cytosolic protein nucleus associated signaling pathways.

Язык: Английский

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A novel multifunctional mitochondrion-targeting NIR fluorophore probe inhibits tumour proliferation and metastasis through the PPARγ/ROS/β-catenin pathway

European Journal of Medicinal Chemistry, Год журнала: 2023, Номер 258, С. 115435 - 115435

Опубликована: Июнь 5, 2023

Язык: Английский

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LGR6 is a potential diagnostic and prognostic marker for esophageal squamous cell carcinoma

Journal of Clinical Laboratory Analysis, Год журнала: 2020, Номер 34(4)

Опубликована: Янв. 9, 2020

Abstract Background Leucine‐rich repeat–coupled receptor 6 (LGR6) is a marker of the skin, nails, and other types adult tissue stem cells has been widely found to be related development progression variety cancer types. The clinical significance biological function LGR6 in esophageal squamous cell carcinoma (ESCC) have not determined. Methods expression at transcriptional level was analyzed by searching TCGA UCSC data sets. Immunohistochemistry, WB, q‐PCR were used detect ESCC adjacent normal tissues. PPI networks KEGG pathways analyze potential functions LGR6. Results tissues significantly higher than that negatively correlated with differentiation degree prognosis patients but closely TNM stage ESCC. showed had close interaction RSPO1, RSPO2, RSPO3, RSPO4. pathway analysis activated Wnt/β‐catenin signaling binding RSPO ligands promote Conclusion can serve as diagnostic prognostic for

Язык: Английский

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hsa_circ_0000285 sponging miR-582-3p promotes neuroblastoma progression by regulating the Wnt/β-catenin signaling pathway

Open Medicine, Год журнала: 2023, Номер 18(1)

Опубликована: Янв. 1, 2023

Abstract Circular RNA has been reported to play a key role in neuroblastoma (NB); however, the of circ_0000285 NB remains unclear. The aim this study was elucidate NB. We studied expression patterns miR-582-3p and tissues cells using real-time quantitative polymerase chain reaction. proteins associated with apoptosis (Bax Bcl-2) Wnt/β-catenin (Wnt, p-Gsk-3β, Gsk-3β, β-catenin, C-myc) were quantified by western blotting. In vivo animal models prepared for functional verification on tumor growth. potential binding ascertained dual-luciferase reporter RNA-binding protein immunoprecipitation experiments. Noticeable upregulation observed samples cell lines. vitro experiments indicated that absence repressed proliferation migration, provoked apoptosis, impaired activity signaling. is targeted poorly expressed cells. additional repression after silencing largely recovered silencing-suppressed migration enhanced apoptosis. restored signaling knockdown circ_0000285. functions as an sponge strengthen activity, thus exacerbating development.

Язык: Английский

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Targeting Features of Curaxin CBL0137 on Hematological Malignancies In Vitro and In Vivo

Biomedicines, Год журнала: 2023, Номер 11(1), С. 230 - 230

Опубликована: Янв. 16, 2023

The anticancer activity of Curaxin CBL0137, a DNA-binding small molecule with chromatin remodulating effect, has been demonstrated in different cancers. Herein, comparative evaluation CBL0137 was performed respect to acute myeloid leukemia (AML), lymphoblastic (ALL), chronic and multiple myeloma (MM) cultured vitro. MTT assay showed AML MM higher sensitivity CBL0137's cytostatic effect comparatively other hematological malignancy cells. Flow cytometry cell cycle analysis revealed an increase subG1 G2/M populations after treatment, but the prevalent type arrest varied. Apoptosis activation by measured Annexin-V/PI dual staining more active RT2 PCR array that changes caused signaling pathways involved cancer pathogenesis were intensive On murine model WEHI-3, significant effects vivo, which evaluated corresponding spleen liver. Thus, pronounced vitro observed MM. Experiments vivo also indicated perspective use for treatment. This accordance frontline treatment approach using epigenetic drugs.

Язык: Английский

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CCNDBP1, a Prognostic Marker Regulated by DNA Methylation, Inhibits Aggressive Behavior in Dedifferentiated Liposarcoma via Repressing Epithelial Mesenchymal Transition

Frontiers in Oncology, Год журнала: 2021, Номер 11

Опубликована: Сен. 22, 2021

The present study aimed to explore the prognostic value, function, and mechanism of CCNDBP1 in dedifferentiated liposarcoma (DDL). Immunohistochemistry staining was used analyze protein expression tissue specimens. After silencing LPS853 overexpressing LPS510, CCK-8, clone formation, transwell migration, invasion assays were detect cell proliferation, ability. CCNDBP1-induced apoptosis analyzed by flow cytometry. altered epithelial-mesenchymal transition (EMT)-related proteins detected Western blot. methylation, gene expression, clinical data 58 samples with DDL using cancer genome atlas (TCGA) database. Low associated a poor prognosis patients considered an independent factor progression-free survival (PFS). significantly inhibited cells vitro promoted apoptosis. could repress pathological EMT, thereby inhibiting malignant behaviors cells. high degree DNA methylation sites cg05194114 cg22184989 decrease worsen patients. This is first reporting that tumor suppressor putative marker might inhibit ability proliferation repressing be regulated DDL.

Язык: Английский

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Puerarin induces platinum-resistant epithelial ovarian cancer cell apoptosis by targeting SIRT1

Journal of International Medical Research, Год журнала: 2021, Номер 49(9)

Опубликована: Сен. 1, 2021

Objective Previous investigations indicated the anticancer activity of puerarin. The current study aimed to evaluate effect and molecular mechanisms puerarin in chemotherapy-resistant ovarian cancer cells. Methods We examined effects platinum-resistant epithelial cells vitro vivo. also analyzed mechanism underlying Wnt/β-catenin inhibition sirtuin 1 (SIRT1) regulation following treatment. Results Our demonstrated that effectively inhibited cell growth vivo by increasing apoptosis More importantly, sensitized cisplatin-resistant chemotherapy. Puerarin treatment decreased SIRT1 expression, which attenuated nuclear accumulation β-catenin inhibit signaling. In addition, overexpression diminished on Further analysis supported SIRT1/β-catenin expression as a candidate biomarker for disease progression cancer. Conclusions increased is partly related downregulation subsequent

Язык: Английский

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