Journal of Clinical Medicine,
Journal Year:
2022,
Volume and Issue:
11(15), P. 4560 - 4560
Published: Aug. 4, 2022
Background:
Cutaneous
melanoma
is
a
heterogeneous
tumor
with
rapidly
switching
molecular
and
cellular
phenotype.
The
invasive
phenotype
characterized
by
MITFlow/AXLhigh
predicts
early
resistance
to
multiple
targeted
drugs
in
melanoma.
Celecoxib
proved
be
valuable
adjuvant
cutaneous
preclinical
studies.
Our
vitro
study
evaluated
for
the
first
time
whether
celecoxib
could
prevent
two
human
cell
lines
treated
dabrafenib.
Methods:
All
experiments
were
carried
out
on
BRAF-V600E-positive
A375
SK-MEL-28
lines,
subjected
dabrafenib
drug
combination
72
h.
Melanoma
cells
already
end
of
spectrum.
Of
main
interest
was
evaluation
key
proteins
expressed
(TGF-β,
MITF,
AXL,
YAP,
TAZ),
as
well
death
mechanisms
correlated
oxidative
stress
production.
Results:
significantly
enhanced
apoptotic
effect
each
line
compared
group
(p
<
0.0001).
Even
though
promoted
low
MITF
expression,
this
high
receptor
tyrosine
kinase
AXL
levels
0.0001),
positive
marker
switch
an
state.
Conclusion:
This
preliminary
highlighted
that
might
promote
expression
dabrafenib,
facilitating
vitro.
results
need
further
confirmation,
finding
represent
important
limitation
antineoplastic
drug.
Analytical Cellular Pathology,
Journal Year:
2021,
Volume and Issue:
2021, P. 1 - 12
Published: March 30, 2021
MicroRNAs
(miRNAs)
regulate
multiple
cellular
behaviors,
and
their
aberrant
expression
is
frequently
associated
with
disease
progression.
This
research
focused
on
the
effects
of
miR-520a
development
non-small-cell
lung
cancer
(NSCLC)
molecules
involved.
Tumor
normal
tissues
from
24
patients
NSCLC
were
collected.
Differentially
expressed
miRNAs
between
tumor
screened
using
microarrays,
was
to
be
significantly
poorly
in
samples.
Artificial
upregulation
reduced
proliferation,
migration
invasion,
resistance
death
A549
H460
cells
according
MTT,
EdU
labeling,
transwell,
flow
cytometry
assays,
respectively.
suppressed
growth
metastasis
xenograft
tumors
vivo.
The
integrated
bioinformatic
analysis
dual
luciferase
assays
suggested
that
targeted
ribonucleotide
reductase
subunit
2
(RRM2)
mRNA
inactivated
Wnt/β-catenin
signaling
pathway
cells.
Upregulation
RRM2
enhanced
malignant
behaviors
NSCLCs,
but
oncogenic
blocked
upon
overexpression.
To
conclude,
this
study
evidenced
inhibits
progression
through
suppressing
Wnt
pathway.
paper
may
offer
novel
insights
into
treatment.
Molecular Medicine Reports,
Journal Year:
2021,
Volume and Issue:
25(1)
Published: Nov. 10, 2021
Recently
accumulated
evidence
has
indicated
that
the
nucleomembrane
shuttling
of
cellular
proteins
is
common,
which
provides
new
insight
into
subcellular
translocation
and
biological
functions
synthesized
in
cytoplasm.
The
present
study
aimed
to
clarify
trafficking
between
plasma
membrane
nucleus.
These
primarily
consist
transmembrane
receptors,
adaptor
proteins,
adhesive
signal
nuclear
contribute
proliferation,
apoptosis,
chemoresistance,
adhesion,
migration
gene
expression.
frequently
undergo
cross‑talk,
such
as
interaction
with
proteins.
endocytosis,
infusion
organelles
or
proteolysis
soluble
forms
for
import
nucleus,
while
interact
act
receptors.
nucleocytosolic
involves
export
through
pores
by
importin
exportin.
Nuclear
generally
other
transcription
factors,
then
binding
promoter
expression,
are
responsible
initiation
and/or
Protein
occurs
a
cell‑specific
manner
closely
linked
events.
review
improve
understanding
cytosolic
protein
nucleus
associated
signaling
pathways.
Journal of Clinical Laboratory Analysis,
Journal Year:
2020,
Volume and Issue:
34(4)
Published: Jan. 9, 2020
Abstract
Background
Leucine‐rich
repeat–coupled
receptor
6
(LGR6)
is
a
marker
of
the
skin,
nails,
and
other
types
adult
tissue
stem
cells
has
been
widely
found
to
be
related
development
progression
variety
cancer
types.
The
clinical
significance
biological
function
LGR6
in
esophageal
squamous
cell
carcinoma
(ESCC)
have
not
determined.
Methods
expression
at
transcriptional
level
was
analyzed
by
searching
TCGA
UCSC
data
sets.
Immunohistochemistry,
WB,
q‐PCR
were
used
detect
ESCC
adjacent
normal
tissues.
PPI
networks
KEGG
pathways
analyze
potential
functions
LGR6.
Results
tissues
significantly
higher
than
that
negatively
correlated
with
differentiation
degree
prognosis
patients
but
closely
TNM
stage
ESCC.
showed
had
close
interaction
RSPO1,
RSPO2,
RSPO3,
RSPO4.
pathway
analysis
activated
Wnt/β‐catenin
signaling
binding
RSPO
ligands
promote
Conclusion
can
serve
as
diagnostic
prognostic
for
Open Medicine,
Journal Year:
2023,
Volume and Issue:
18(1)
Published: Jan. 1, 2023
Abstract
Circular
RNA
has
been
reported
to
play
a
key
role
in
neuroblastoma
(NB);
however,
the
of
circ_0000285
NB
remains
unclear.
The
aim
this
study
was
elucidate
NB.
We
studied
expression
patterns
miR-582-3p
and
tissues
cells
using
real-time
quantitative
polymerase
chain
reaction.
proteins
associated
with
apoptosis
(Bax
Bcl-2)
Wnt/β-catenin
(Wnt,
p-Gsk-3β,
Gsk-3β,
β-catenin,
C-myc)
were
quantified
by
western
blotting.
In
vivo
animal
models
prepared
for
functional
verification
on
tumor
growth.
potential
binding
ascertained
dual-luciferase
reporter
RNA-binding
protein
immunoprecipitation
experiments.
Noticeable
upregulation
observed
samples
cell
lines.
vitro
experiments
indicated
that
absence
repressed
proliferation
migration,
provoked
apoptosis,
impaired
activity
signaling.
is
targeted
poorly
expressed
cells.
additional
repression
after
silencing
largely
recovered
silencing-suppressed
migration
enhanced
apoptosis.
restored
signaling
knockdown
circ_0000285.
functions
as
an
sponge
strengthen
activity,
thus
exacerbating
development.
Biomedicines,
Journal Year:
2023,
Volume and Issue:
11(1), P. 230 - 230
Published: Jan. 16, 2023
The
anticancer
activity
of
Curaxin
CBL0137,
a
DNA-binding
small
molecule
with
chromatin
remodulating
effect,
has
been
demonstrated
in
different
cancers.
Herein,
comparative
evaluation
CBL0137
was
performed
respect
to
acute
myeloid
leukemia
(AML),
lymphoblastic
(ALL),
chronic
and
multiple
myeloma
(MM)
cultured
vitro.
MTT
assay
showed
AML
MM
higher
sensitivity
CBL0137's
cytostatic
effect
comparatively
other
hematological
malignancy
cells.
Flow
cytometry
cell
cycle
analysis
revealed
an
increase
subG1
G2/M
populations
after
treatment,
but
the
prevalent
type
arrest
varied.
Apoptosis
activation
by
measured
Annexin-V/PI
dual
staining
more
active
RT2
PCR
array
that
changes
caused
signaling
pathways
involved
cancer
pathogenesis
were
intensive
On
murine
model
WEHI-3,
significant
effects
vivo,
which
evaluated
corresponding
spleen
liver.
Thus,
pronounced
vitro
observed
MM.
Experiments
vivo
also
indicated
perspective
use
for
treatment.
This
accordance
frontline
treatment
approach
using
epigenetic
drugs.
Journal of International Medical Research,
Journal Year:
2021,
Volume and Issue:
49(9)
Published: Sept. 1, 2021
Objective
Previous
investigations
indicated
the
anticancer
activity
of
puerarin.
The
current
study
aimed
to
evaluate
effect
and
molecular
mechanisms
puerarin
in
chemotherapy-resistant
ovarian
cancer
cells.
Methods
We
examined
effects
platinum-resistant
epithelial
cells
vitro
vivo.
also
analyzed
mechanism
underlying
Wnt/β-catenin
inhibition
sirtuin
1
(SIRT1)
regulation
following
treatment.
Results
Our
demonstrated
that
effectively
inhibited
cell
growth
vivo
by
increasing
apoptosis
More
importantly,
sensitized
cisplatin-resistant
chemotherapy.
Puerarin
treatment
decreased
SIRT1
expression,
which
attenuated
nuclear
accumulation
β-catenin
inhibit
signaling.
In
addition,
overexpression
diminished
on
Further
analysis
supported
SIRT1/β-catenin
expression
as
a
candidate
biomarker
for
disease
progression
cancer.
Conclusions
increased
is
partly
related
downregulation
subsequent
